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POC SPLA2-IIA as a Biomarker for Sepsis and Septic Shock

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Know the risks and potential benefits of clinical studies and talk to your health care provider before participating. Read our disclaimer for details. Identifier: NCT03953404
Recruitment Status : Recruiting
First Posted : May 16, 2019
Last Update Posted : May 17, 2019
Information provided by (Responsible Party):
rebecca jeanmonod, St. Luke's Hospital, Pennsylvania

Brief Summary:

Septic shock is a major cause of morbidity and mortality. SIRS (systemic inflammatory response syndrome) can progress over hours to days to severe sepsis and septic shock. Currently, lactate levels are used to guide resuscitative efforts and have been shown to be a predictor of mortality independent of vital sign abnormalities (1). However, their use seems to be limited to trending in a given patient, and not for prognostic value of a single level (2). This is because there is significant overlap in lactate levels of individuals who progress to death and multisystem organ failure as compared to those who do not (2). Blood cultures are also extensively used to detect blood stream infection (BSI), but these are time consuming and are not immediately useful to clinicians caring for sick patients.

A biomarker that adequately distinguishes between patients at high risk for progression to severe sepsis/shock/death and those who will not would be helpful in the appropriate initiation of aggressive treatment and appropriate disposition of patients in clinical care. Previously, the investigators demonstrated that sPLA2-IIA detected by ELISA assay had a sensitivity of 87% and a specificity of 91% in detecting sepsis (3). Zeus Pharmaceuticals has developed a bedside point-of-care test measuring sPLA2-IIA in real time. The investigators propose to study this assay in terms of its discriminatory value in distinguishing between SIRS from non-infectious causes, sepsis, severe sepsis, and septic shock in a cohort of patients presenting to the emergency department at Anderson and Bethlehem campuses. The investigators propose to better define the threshold level for this marker assay as well as seek to establish its utility in a clinical population.

The investigators will take samples of blood from emergency department patients presenting who meet SIRS criteria or have a positive q-SOFA screen. The investigators will take subsequent samples of blood when lactate levels are redrawn as per St. Luke's sepsis protocol. After informed consent is obtained, blood specimens will be run in analyzer provided by Zeus for sPLA2-IIA. The investigators will record presence and quantity of sPLA2-IIA, as well as other markers of sepsis such as lactate, vital signs, blood cultures, and patient oriented outcomes (ie ICU days, organ dysfunction, and survival to discharge). Printouts from analyzer will be stored in locked cabinet, and remaining blood will be discarded. The data will then be compiled by the investigators at St. Luke's University Hospital. The results will be correlated with the patients' clinical progression to determine the biomarker's utility and cut-off values for predicting progression of SIRS.

As clear threshold levels for this marker have yet to be defined, the investigators would like to enroll patients meeting criteria until the investigators have enrolled 50 patients with septic shock. It is anticipated that, proportionally, this will lead to enrollment of 75-100 patients with severe sepsis, 100-150 patients with sepsis, and 100-150 patients meeting SIRS criteria who are not septic. This will help delineate if there is any value in this assay for distinguishing among the severity of sepsis pathophysiology.

Condition or disease Intervention/treatment
Sepsis Diagnostic Test: SPLA2-IIA

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Study Type : Observational
Estimated Enrollment : 450 participants
Observational Model: Cohort
Time Perspective: Prospective
Official Title: Bedside Measurements of a Novel Biomarker SPLA2-IIA as a Marker of an Inflammatory Response and Predictor of Sepsis in Patients Meeting SIRS or qSOFA Criteria: a Pilot Study
Actual Study Start Date : May 14, 2019
Estimated Primary Completion Date : April 30, 2020
Estimated Study Completion Date : April 30, 2022

Resource links provided by the National Library of Medicine

MedlinePlus related topics: Sepsis Shock

Group/Cohort Intervention/treatment
septic shock Diagnostic Test: SPLA2-IIA
bedside blood test

severe sepsis Diagnostic Test: SPLA2-IIA
bedside blood test

sepsis Diagnostic Test: SPLA2-IIA
bedside blood test

sirs positive, not septic Diagnostic Test: SPLA2-IIA
bedside blood test

Primary Outcome Measures :
  1. level greater than 25ng [ Time Frame: baseline ]
    threshold level of spla2-IIA

  2. level greater than 25ng [ Time Frame: 2 hours ]
    threshold level of spla2-IIA

  3. level greater than 25ng [ Time Frame: 24 hour lab draw ]
    threshold level of spla2-IIA

Information from the National Library of Medicine

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Ages Eligible for Study:   18 Years and older   (Adult, Older Adult)
Sexes Eligible for Study:   All
Sampling Method:   Probability Sample
Study Population
emergency department patients with positive sepsis screens

Inclusion Criteria:

  • presenting to emergency department with positive sepsis screen including 2 SIRS criteria or positive q-sofa score regardless of reasons for positive screen

Exclusion Criteria:

  • inability to consent or to have surrogate consent, identification after resuscitation or after blood has already been drawn, age less than 18, pregnant

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT03953404

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United States, Pennsylvania
St. Luke'S University Health Network Recruiting
Bethlehem, Pennsylvania, United States, 18015
Contact: Rebecca Jeanmonod, MD    610-838-6147   
Contact: Donald Jeanmonod, MD    610 500 3733   
Sponsors and Collaborators
St. Luke's Hospital, Pennsylvania
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Responsible Party: rebecca jeanmonod, Professor of Emergency Medicine, St. Luke's Hospital, Pennsylvania Identifier: NCT03953404    
Other Study ID Numbers: SLIR 2019-23
First Posted: May 16, 2019    Key Record Dates
Last Update Posted: May 17, 2019
Last Verified: May 2019
Individual Participant Data (IPD) Sharing Statement:
Plan to Share IPD: No

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Studies a U.S. FDA-regulated Drug Product: No
Studies a U.S. FDA-regulated Device Product: No
Additional relevant MeSH terms:
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Systemic Inflammatory Response Syndrome
Pathologic Processes