Effect of Fentanyl on Main Opioid Receptor (OPRM1) on Human Granulosa Cells.
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|ClinicalTrials.gov Identifier: NCT03248076|
Recruitment Status : Unknown
Verified December 2017 by Asouhidou Irene, George Papanicolaou Hospital.
Recruitment status was: Recruiting
First Posted : August 14, 2017
Last Update Posted : December 14, 2017
Opioids is known that produce not only analgesia but also hyperalgesia through activation of central glutaminergic system-GABA. At the same time, recently it was found that the main opioid receptor (OPRM1) is present on human granulosa cells and exogenous opiates and their antagonists can influence granulosa cell vascular endothelial growth factor (VEGF) production via OPRM1, causing ovarian hyperstimulation syndrome.
This study aims to investigate if a single exposure to opioids is enough to produce activation of stress mechanism during oocyte retrieval.
|Condition or disease||Intervention/treatment|
|Stress Reaction Opioid Use||Drug: Fentanyl Citrate|
The main opioid receptor (OPRM1) is present on human granulosa cells and exogenous opiates and their antagonists can influence granulosa cell vascular endothelial growth factor (VEGF) production via OPRM1, causing ovarian hyperstimulation syndrome.
This study aims to investigate if a single exposure to opioids is enough to produce activation of stress mechanism during ultrasound-guided oocyte retrieval. It will be measured the level of cortisone before and 15min after iv administration of fentanyl on blood and on oocyte fluid.
|Study Type :||Observational|
|Estimated Enrollment :||30 participants|
|Official Title:||Effect of Fentanyl on Expression of Main Opioid Receptor (OPRM1) on Human Granulosa Cells During Ultrasound-guided Transvaginal Oocyte Retrieval.|
|Actual Study Start Date :||April 1, 2017|
|Estimated Primary Completion Date :||August 31, 2018|
|Estimated Study Completion Date :||October 10, 2018|
Baseline blood sample and oocyte fluid will be collected under propofol anesthesia. Fifteen minutes after administration of 1γ/kgfentanyl, it will be collected again blood sample and oocyte fluid. Cortisone and fentanyl level will be measured in all samples.
Drug: Fentanyl Citrate
- fentanyl [ Time Frame: 15 minutes ]fentanyl on blood sample and oocyte fluid
- cortisone [ Time Frame: 15 min ]cortisone on blood sample and oocyte fluid
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT03248076
|Contact: Irene Asouhidou, MD, PhDemail@example.com|
|Contact: Evaggelos Papanikolaou, MD, PhDfirstname.lastname@example.org|
|Section of Anatomy, Aristotle University of Thessaloniki||Recruiting|
|Thessaloníki, Greece, 54124|
|Contact: Irene Asouhidou, MD, PhD 2310999321 email@example.com|
|Contact: Evaggelos Papanikolaou, MD, PhD +302310424294 firstname.lastname@example.org|
|Principal Investigator:||Irene Asouhidou, MD, PhD||Assisting Nature|