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Human Brown Adipose Tissue and Mitochondrial Respiration

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Know the risks and potential benefits of clinical studies and talk to your health care provider before participating. Read our disclaimer for details. Identifier: NCT03111719
Recruitment Status : Recruiting
First Posted : April 13, 2017
Last Update Posted : February 21, 2020
Ministry of Education, Culture and Science
Information provided by (Responsible Party):
Maastricht University Medical Center

Brief Summary:
This study is focused on measuring mitochondrial activity of human brown adipose tissue. During surgery, adipose tissue biopsies from white and brown adipose tissue will be collected from the deep neck area. These biopsies will be used to examine mitochondrial function via histological analysis, biochemical analysis and in vitro experiments based on precursor cells present in the biopsies.

Condition or disease
Mitochondrial Metabolism Disorders Adipose Tissue, Brown Aging Obesity

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Study Type : Observational
Estimated Enrollment : 180 participants
Observational Model: Cohort
Time Perspective: Cross-Sectional
Official Title: Human Brown Adipose Tissue Histological and Mitochondrial Respiratory Analysis
Actual Study Start Date : January 4, 2010
Estimated Primary Completion Date : October 10, 2021
Estimated Study Completion Date : October 10, 2021

Primary Outcome Measures :
  1. Mitochondrial respiration in human brown [ Time Frame: 8 weeks ]
    Measure of mitochondrial respiration in human brown adipose tissue biopsies and measure mitochondrial respiration in cells derived from human brown adipose tissue biopsies. With high resolution respirometry , oxygen consumption in mitochondria will be determined using different substrates that stimulate mitochondrial activity including norepinephrine which is a natural agonist to increase mitochondrial activity in brown adipose tissue. During these experiments mitochondrial complex activity can be inhibited using specific inhibitors.

Secondary Outcome Measures :
  1. Characterise human brown adipose tissue [ Time Frame: 8 weeks ]
    Perform histological analysis and biochemical analysis in brown adipose tissue biopsies and compare to skeletal muscle. Via qPCR analysis, western blotting and immunfluorescence we will examine UCP1 expression in BAT.

Information from the National Library of Medicine

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Ages Eligible for Study:   18 Years to 65 Years   (Adult, Older Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   No
Sampling Method:   Non-Probability Sample
Study Population
Patients indicated for surgery in the supraclavicular and neck region

Inclusion Criteria:

  • Indication for surgery in the supraclavicular and neck region (more specific patients diagnosed with hyperparathyroidism, struma, benign thyroid gland tumours, implantation of a vagal neurostimulator for epilepsia and cervical neurologic disorders)
  • In the thyroid gland group, euthyroid function

Exclusion Criteria:

  • Pre-operatively diagnosed malign tumour
  • Patients undergoing acute surgery because of critical illness

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT03111719

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Contact: Wouter D Van Marken Lichtenbelt, Professor +31(0)433881629

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Maastricht University Medical Center / University of Maastricht Recruiting
Maastricht, Netherlands
Contact: Wouter D Van Marken Lichtenbelt, Professor    +31(043)3881629   
Sponsors and Collaborators
Maastricht University Medical Center
Ministry of Education, Culture and Science
Publications automatically indexed to this study by Identifier (NCT Number):
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Responsible Party: Maastricht University Medical Center Identifier: NCT03111719    
Other Study ID Numbers: NL31367.068.10
First Posted: April 13, 2017    Key Record Dates
Last Update Posted: February 21, 2020
Last Verified: February 2020
Additional relevant MeSH terms:
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Metabolic Diseases