Human Brown Adipose Tissue and Mitochondrial Respiration
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|ClinicalTrials.gov Identifier: NCT03111719|
Recruitment Status : Recruiting
First Posted : April 13, 2017
Last Update Posted : February 21, 2020
|Condition or disease|
|Mitochondrial Metabolism Disorders Adipose Tissue, Brown Aging Obesity|
|Study Type :||Observational|
|Estimated Enrollment :||180 participants|
|Official Title:||Human Brown Adipose Tissue Histological and Mitochondrial Respiratory Analysis|
|Actual Study Start Date :||January 4, 2010|
|Estimated Primary Completion Date :||October 10, 2021|
|Estimated Study Completion Date :||October 10, 2021|
- Mitochondrial respiration in human brown [ Time Frame: 8 weeks ]Measure of mitochondrial respiration in human brown adipose tissue biopsies and measure mitochondrial respiration in cells derived from human brown adipose tissue biopsies. With high resolution respirometry , oxygen consumption in mitochondria will be determined using different substrates that stimulate mitochondrial activity including norepinephrine which is a natural agonist to increase mitochondrial activity in brown adipose tissue. During these experiments mitochondrial complex activity can be inhibited using specific inhibitors.
- Characterise human brown adipose tissue [ Time Frame: 8 weeks ]Perform histological analysis and biochemical analysis in brown adipose tissue biopsies and compare to skeletal muscle. Via qPCR analysis, western blotting and immunfluorescence we will examine UCP1 expression in BAT.
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT03111719
|Contact: Wouter D Van Marken Lichtenbelt, Professor||+31(0)email@example.com|
|Maastricht University Medical Center / University of Maastricht||Recruiting|
|Contact: Wouter D Van Marken Lichtenbelt, Professor +31(043)3881629 firstname.lastname@example.org|