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Effects of Sleep Deprivation on Blood Hormones and Inflammatory Status After Exercise Induced-muscle Damage

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ClinicalTrials.gov Identifier: NCT02082600
Recruitment Status : Unknown
Verified March 2014 by Hanna Karen Moreira Antunes, Federal University of São Paulo.
Recruitment status was:  Active, not recruiting
First Posted : March 10, 2014
Last Update Posted : March 11, 2014
Sponsor:
Collaborator:
Fundação de Amparo à Pesquisa do Estado de São Paulo
Information provided by (Responsible Party):
Hanna Karen Moreira Antunes, Federal University of São Paulo

Brief Summary:
The aims of this study are examine the effects of sleep deprivation in muscle recovery after a maximum eccentric resistance exercise session performed on an isokinetic dynamometer (24 series of 10 repetitions). The sample will consist of 10 men, sedentary, clinically healthy, aged between 20 and 31 years old. Two experimental groups will be developed: EXE-SLEEP, in which subjects will perform the exercise protocol (~18:00-19:00) and will be subject to normal period of sleep for 3 nights; EXE-TOTAL, in which subjects will perform the same exercise protocol and will be sleep deprived for 60 hours, followed by one night of sleep rebound.

Condition or disease Intervention/treatment Phase
Sleep Sleep Deprivation Other: Sleep deprivation Other: Normal sleep Not Applicable

Detailed Description:

The blood parameters analyzed will be:

  • Creatine kinase and myoglobin: before, immediately after, 2, 4, 12, 24, 36, 48, and 60 hours after exercise protocol.
  • Total and free testosterone, IGF-1, and cortisol: before, immediately after, 2, 4, 12, 24, 36, 48, and 60 hours after exercise protocol. Moreover, from 19:00 of the second day to 19:00 of the third day it will be collected blood samples every 2 hours.
  • GH: during the second night, GH will be evaluated every 1 hour, from 23:00 to 07:00.
  • TNF-alfa, IL-6, IL-1 beta, IL-10, and IL-1ra: before, immediately after, and 2 hour after exercise, as well every 4 hours from 19:00 of the second day to 19:00 of the third day.

It will be evaluated isometric strength every 12 hours, as well visual analog scale of pain.


Study Type : Interventional  (Clinical Trial)
Actual Enrollment : 10 participants
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)
Primary Purpose: Basic Science
Official Title: Effects of Sleep Deprivation on Blood Hormones and Inflammatory Status After Exercise Induced-muscle Damage
Study Start Date : July 2013
Actual Primary Completion Date : December 2013

Resource links provided by the National Library of Medicine


Arm Intervention/treatment
Experimental: Sleep deprivation
Exercise induced-muscle damage protocol followed by 60h of sleep deprivation (two nights) and one night of normal sleep (rebound).
Other: Sleep deprivation
This group will perform 24 sets of 10 maximum repetitions of eccentric contractions for quadriceps and than will keep awake for 60 hours (2 nights), followed by 1 night of sleep, all inside the lab. During whole protocol, it will be collected several blood samples for evaluation of hormones, cytokines and muscle damage parameters. It will be also evaluated isometric strength and pain.
Other Name: Sleep and muscle recovery

Experimental: Normal sleep
Exercise induced-muscle damage protocol followed by 3 nights of normal sleep
Other: Normal sleep
This group will perform 24 sets of 10 maximum repetitions of eccentric contractions for quadriceps and and will be able to sleep the 3 nights. During whole protocol, it will be collected several blood samples for evaluation of hormones, cytokines and muscle damage parameters. It will be also evaluated isometric strength and pain.




Primary Outcome Measures :
  1. Change from baseline in isometric strength [ Time Frame: Before exercise, immediatly after, 12, 24, 36, 48, and 60 hours after exercise induced-muscle damage ]

Secondary Outcome Measures :
  1. Area Under Curve for serum total and free testosterone, IGF-1, and cortisol [ Time Frame: Blood samples will be collected every 2 hours, for 24 hours. ]

Other Outcome Measures:
  1. Area Under Curve for serum pro and anti-inflammatory cytokines [ Time Frame: Blood samples will be collected every 4 hours, for 24 hours. ]


Information from the National Library of Medicine

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Ages Eligible for Study:   20 Years to 31 Years   (Adult)
Sexes Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes
Criteria

Inclusion Criteria:

  • healthy subjects
  • sedentary
  • sleep duration between 6-8 hours peer night

Exclusion Criteria:

  • smokers
  • use vitamin supplements, like C and E
  • drink two doses of alcohol/day or > 4 doses in one occasion
  • use of anti-inflammatory drugs
  • antilipidemic drugs
  • anemia
  • muscle or joint diseases
  • cardiovascular diseases
  • diabetes
  • renal diseases
  • sleep disturbances
  • breathing disorders

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT02082600


Locations
Brazil
Associação Fundo de Incentivo à Pesquisa
Sao Paulo, Brazil, 04020-050
Sponsors and Collaborators
Federal University of São Paulo
Fundação de Amparo à Pesquisa do Estado de São Paulo
Investigators
Principal Investigator: Marco Tulio Mello, PhD Federal University of Sao Paulo

Responsible Party: Hanna Karen Moreira Antunes, PhD, Federal University of São Paulo
ClinicalTrials.gov Identifier: NCT02082600     History of Changes
Other Study ID Numbers: 2010070050
First Posted: March 10, 2014    Key Record Dates
Last Update Posted: March 11, 2014
Last Verified: March 2014

Additional relevant MeSH terms:
Sleep Deprivation
Dyssomnias
Sleep Wake Disorders
Nervous System Diseases
Neurologic Manifestations
Signs and Symptoms
Mental Disorders
Hormones
Hormones, Hormone Substitutes, and Hormone Antagonists
Physiological Effects of Drugs