Studying Samples From Patients With T-Cell Acute Lymphoblastic Leukemia

The recruitment status of this study is unknown because the information has not been verified recently.
Verified April 2012 by National Cancer Institute (NCI).
Recruitment status was  Active, not recruiting
Information provided by:
National Cancer Institute (NCI) Identifier:
First received: April 19, 2012
Last updated: April 21, 2012
Last verified: April 2012

RATIONALE: Studying samples of blood, tissue, and bone marrow from patients with cancer in the laboratory may help doctors identify learn more about biomarkers related to cancer. It may also help doctors to find better ways to treat cancer.

PURPOSE: This research studies samples from patients with T-cell acute lymphoblastic leukemia (T-ALL).

Condition Intervention
Genetic: gene expression analysis
Other: cell culture procedure
Other: flow cytometry
Other: laboratory biomarker analysis
Other: metabolic assessment

Study Type: Observational
Official Title: Metabolic Pathways in T-Cell Acute Lymphoblastic Leukemia (T-ALL)

Resource links provided by NLM:

Further study details as provided by National Cancer Institute (NCI):

Primary Outcome Measures:
  • Metabolic status of primary T-ALL [ Designated as safety issue: No ]
  • Effects of metabolic inhibition on metabolic stress pathways and apoptosis [ Designated as safety issue: No ]
  • Metabolic inhibition interaction with chemotherapy or targeted drugs [ Designated as safety issue: No ]

Estimated Enrollment: 15
Study Start Date: April 2012
Estimated Primary Completion Date: June 2012 (Final data collection date for primary outcome measure)
Detailed Description:


  • Determine the metabolic status and regulation of primary T-cell acute lymphoblastic leukemia (T-ALL) relative to control resting peripheral T cells.
  • Establish the effects of metabolic inhibition on metabolic stress pathways and apoptosis.
  • Determine how metabolic inhibition interacts with chemotherapy or targeted therapy drugs to kill T-ALL cells.

OUTLINE: T-ALL samples cultured alone or with gamma secretase inhibitors (GSI) or PI3K inhibitors are analyzed for metabolic characteristics including glucose transporter 1 (Glut1) expression, mitochondrial mass, phospho-flow for 5' adenosine monophosphate-activated protein kinase (AMPK), acetyl-CoA carboxylase (ACC), and mammalian target of rapamycin (mTOR) by flow cytometry. T-ALL samples and normal CD4+ T cells (control) are also exposed to ± 2-deoxyglucose or ± the glutaminolysis inhibitor media and analyzed for metabolic stress responses over time in particular, AMPK activation, autophagy (immunofluorescence for LC3-II processing), and BCL2-associated X protein (Bak) and Bax activation to indicate apoptosis. These cells (T-ALL and control) are then cultured with cyclophosphamide, dexamethasone, or the B-cell CLL/lymphoma 2 (Bcl-2) inhibitor, ABT-737, to determine cell death over time.


Genders Eligible for Study:   Both
Accepts Healthy Volunteers:   Yes


  • Sample from patients diagnosed with T-ALL
  • Samples from independent healthy donors obtained through the Gulf Coast Regional Blood Center (controls)


  • Not specified


  • Not specified
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Please refer to this study by its identifier: NCT01581528

Sponsors and Collaborators
Children's Oncology Group
Principal Investigator: Jeffrey C. Rathmell, PhD Duke Cancer Institute
  More Information

Additional Information:
No publications provided

Responsible Party: Peter C. Adamson, Children's Oncology Group - Group Chair Office Identifier: NCT01581528     History of Changes
Other Study ID Numbers: CDR0000732166, COG-AALL12B5
Study First Received: April 19, 2012
Last Updated: April 21, 2012
Health Authority: United States: Federal Government

Keywords provided by National Cancer Institute (NCI):
T-cell childhood acute lymphoblastic leukemia
untreated adult acute lymphoblastic leukemia
T-cell adult acute lymphoblastic leukemia
untreated childhood acute lymphoblastic leukemia

Additional relevant MeSH terms:
Leukemia, Lymphoid
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
Immune System Diseases
Immunoproliferative Disorders
Lymphatic Diseases
Lymphoproliferative Disorders
Neoplasms by Histologic Type processed this record on March 31, 2015