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Risk Factors and Biomarkers Associated With Recurrence After Excision of Primary Pterygium

This study has been terminated.
(Other and future studies on lipiview in pterygium will cover this current trial sufficiently.)
Singapore Eye Research Institute
Information provided by (Responsible Party):
Louis Tong, Singapore National Eye Centre Identifier:
First received: February 12, 2010
Last updated: May 18, 2015
Last verified: January 2013
This study looks at global gene expression in pterygium and found the derangement of matrix genes in particular to be a feature of pterygium. In the investigators opinion, it would be more beneficial to elicit changes in gene expression before the recurrence of pterygium and by developing a panel of biomarkers that are associated with pterygium recurrence; one would be able to predict the post surgical prognosis of patients after resection.Biomarker levels will be compared to discover a biomarker panel for prediction of recurrence.


Study Type: Observational
Study Design: Observational Model: Cohort
Time Perspective: Prospective
Official Title: Risk Factors and Biomarkers Associated With Recurrence After Excision of Primary Pterygium

Further study details as provided by Louis Tong, Singapore National Eye Centre:

Primary Outcome Measures:
  • biomarkers [ Time Frame: 12 months ]

    Elevation of biomarkers in pterygium tissue and tears (transcripts, proteins, or tear proteins) at the time of initial presentation of primary pterygium may predict the post-surgical recurrence within one year.

    visits: 2 further visits at 6 months and 12 months after surgery

Biospecimen Retention:   Samples With DNA
Pterygium and conjunctival tissue samples

Enrollment: 1
Study Start Date: March 2010
Study Completion Date: December 2014
Primary Completion Date: December 2014 (Final data collection date for primary outcome measure)
Detailed Description:

A comprehensive questionnaire will be used to evaluate the environmental, biological, physical and behavioral risk factors of associated with the recurrence after pterygium excision.

Slit lamp digital color photographs will be taken on primary and recurrent pterygium and pterygium will be graded in three levels of severity, based on relative transparency of pterygium tissue; grade 1 (transparent), grade 2 (intermediate), and grade 3 (opaque) to see whether the risk factors correlate to the morphology of the pterygium.

Tear samples will be collected before surgery using schirmers strip.Proteins in tear samples will be analyzed using the iTRAQ method.

The transcriptional differences in pterygium and conjunctival tissue samples will be analyzed using western blot, qPCR and immunohistochemistry assays.


Ages Eligible for Study:   21 Years and older   (Adult, Senior)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   No
Sampling Method:   Non-Probability Sample
Study Population
Pterygium patients from the investigator's clinic as well as other clinics at the Singapore National Eye Center will be recruited for this study.

Inclusion Criteria:

  • Clinical diagnosis of primary and recurrence pterygium
  • Must agree to participate in this study
  • Must sign informed consent form

Exclusion Criteria:

  • Hepatitis
  Contacts and Locations
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Please refer to this study by its identifier: NCT01068496

Singapore National Eye Centre
Singapore, Singapore, 168571
Singapore National Eye Center
Singapore, Singapore, 168751
Sponsors and Collaborators
Singapore National Eye Centre
Singapore Eye Research Institute
Study Chair: Samanthila Waduthantri Singapore National Eye Centre
Study Chair: Laurence Lim, MBBS Singapore National Eye Centre
Study Chair: Federico Luengo Gimeno Singapore National Eye Centre
Study Chair: Jodhbir Mehta Singapore National Eye Centre
  More Information

Responsible Party: Louis Tong, Singapore National Eye Centre Identifier: NCT01068496     History of Changes
Other Study ID Numbers: R707/57/2009
Study First Received: February 12, 2010
Last Updated: May 18, 2015

Additional relevant MeSH terms:
Disease Attributes
Pathologic Processes
Conjunctival Diseases
Eye Diseases processed this record on May 25, 2017