The Immune Reactivity of Biofilms in Vaginal Mesh Erosion.
|ClinicalTrials.gov Identifier: NCT00564044|
Recruitment Status : Unknown
Verified August 2007 by Chang Gung Memorial Hospital.
Recruitment status was: Active, not recruiting
First Posted : November 27, 2007
Last Update Posted : November 27, 2007
Aging, birth trauma and extensive pelvic surgery are the causes known to cause advanced pelvic organ prolaspe, fecal as well as urinary incontinence. Surgical treatment is the last resort to manage the above-mentioned clinical manifestations of pelvic floor disorders except the subject is too frail to receive operation.
In order to improve the outcome of reconstructive pelvic surgery, reinforcement with synthetic mesh or biological material is the modern trend in pelvic repair. Unfortunately no prosthesis including synthetic or biological is ideal because vaginal erosion with mesh extrusion which is the subject of this protocol and other complications were reported continuously. As per the literature, the rate for mesh vaginal extrusion ranged between 2.4 and 17% when polypropylene which is the most popular synthetic material used for the mid-urethral sling or pelvic reconstructive surgery to date. The causes of this complication are still controversial which include rejection, poor quality of tissue, surgical artifact, material of mesh and etc.
A prospective controlled study for the investigation of the cause for mesh vaginal erosion was conducted and the results revealed evidences of immune reactivity after mesh implantation, albeit the evidence was not solid (Am J Obstet Gynecol 2004; 191(6): 1868-1874 ). As per the pilot study initially done by us to determine the biofilm-related-infection, we have found bacterial biofilm could adhere to surfaces and interfaces, i.e. bacteria located in the cells just beneath the contacting surfaces in the electron microscopic (EM) analysis. In addition, soon after bacteria infection, proteins in biofilm undergo conformational changes, making them immunogenic and triggers a typical inflammatory response leading to activation of the complement system. Thus, we plan to use CD (clusters of differentiation) antigens - 4, 8, 20, 25, 40, 68 and quantitative analysis of FoxP3 to determine the function of regulatory T cells in the immune response. In addition, bacterial culture and EM analysis of the excised mesh with surrounding vagina tissue will be performed for further analysis of biofilms.
|Condition or disease||Intervention/treatment||Phase|
|Uterine Prolapse Urinary Incontinence Fecal Incontinence||Procedure: excision of the protruding mesh and its surrounding vaginal tissue||Not Applicable|
|Study Type :||Interventional (Clinical Trial)|
|Estimated Enrollment :||82 participants|
|Intervention Model:||Parallel Assignment|
|Masking:||Single (Outcomes Assessor)|
|Primary Purpose:||Basic Science|
|Official Title:||Does the Immune Reactivity of Bacteria Cause Vaginal Mesh (Polypropylene) Erosion? - A Ultrastructural, Microbiological and Immunohistochemical Analysis.|
|Study Start Date :||August 2007|
|Estimated Study Completion Date :||July 2009|
|Active Comparator: 2||
Procedure: excision of the protruding mesh and its surrounding vaginal tissue
A piece of vaginal tissue 12mm*5mm*3mm in sized (for control group) and another piece of vaginal tissue combined with protruding mesh of the same size (for study group) will be obtained respectively for each of the two arms during intervention.
- To determine whether bacterial infection with biofilm formation exists in the vaginal tissue with mesh extrusion using bacterial culture and electron microscopic analysis. [ Time Frame: 3 years ]
- With the use of immunohistochemical (IHC) analysis of CD 4, 8, 20, 25, 40, 68 and quantitative analysis of Fox P3 (using RT-POR), to determine the function of regulatory T cells in the immune reactivity of biofilms. [ Time Frame: 3 years ]
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT00564044
|Chang Gung Memorial Hospital|
|Gueishan, Taoyuan, Taiwan, 333|
|Principal Investigator:||Alex Wang, MD||Division of Female Pelvic Medicine and Reconstructive Surgery, Department of OB/GYN, Chang Gung Memorial Hospital|
|Study Chair:||Cheng-Hsun Chiu, MD. PhD||Department of Pediatrics, Chang Gung Memorial Hospital|
|Study Director:||Yu-Shien Ko, MD, PhD||First Cardiovascular Division, Chang Gung Memorial Hospital|
|Study Director:||Cheng-Tao Lin, MD||Division of Gynecological Oncology, Department of OB/GYN, Chang Gung Memorial Hospital|
|Study Director:||Ren-Chin Wu, MD||Department of Surgical Pathology, Chang Gung Memorial Hospital|
|Study Director:||Tsia-Shu Lo, MD||Division of Female Pelvic Medicine and Reconstructive Surgery, Department of OB/GYN, Chang Gung Memorial Hospital|
|Study Director:||Min-Chi Chen, PhD||Biostatistics Center and Department of Public Health, Chang Gung University|
|Study Director:||Yi-Haou Lin, MD||Division of Female Pelvic Medicine and Reconstructive Surgery, Department of OB/GYN, Chang Gung Memorial Hospital|