A Search for Helicobacter Pylori in Localized Vulvodynia
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|ClinicalTrials.gov Identifier: NCT00501774|
Recruitment Status : Completed
First Posted : July 16, 2007
Last Update Posted : July 16, 2007
|Condition or disease|
|Vulvodynia Vulvar Vestibulitis|
Subsequent sections from the paraffin blocks were prepared and stained by modified Giemsa. Immunostaining for H. pylori was done as described. In short, tissue sections were deparaffinized in xylene, rehydrated through decreasing concentrations of alcohol ending in phosphate-buffered saline (PBS), and subjected to pretreatment with Proteinase K (8 minutes). The sections were quenched with 3% hydrogen peroxidase, incubated with protein block for 15 minutes at room temperature, and washed in PBS. Tissues then were incubated with polyclonal rabbit anti-H pylori antibody (dilution, 1:10; clone ch-20 429, DAKO, Carpinteria, CA). Finally, sections were washed in 0.05% polysorbate 20 in PBS, pH 7.4, and the bound antibody was detected using streptavidin and biotinylated secondary antibody with diaminobenzidine as the chromogen. Sections were counterstained with hematoxylin, dehydrated, and mounted. Negative controls were sections treated as above, but instead of incubation with the primary antibody, they were incubated with 1% bovine serum albumin in PBS.
Vulvar biopsies of seven other women without localized vulvodynia served as healthy controls.
The positive and negative control gastric tissues for the immunohistochemical stain of the H. pylori microorganisms were obtained from the archives of the Department of Pathology.
|Study Type :||Observational|
|Actual Enrollment :||27 participants|
|Observational Model:||Case Control|
|Observational Model:||Natural History|
|Official Title:||A Laboratory and Clinical Search for the Presence of H. Pylori and Treatment Against it in Localized Vulvodynia (Vestibulitis, Vestibulodynia).|
|Study Start Date :||May 2004|
|Actual Study Completion Date :||June 2007|
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT00501774
|Principal Investigator:||Jacob Bornstein, MD||westen Galilee Hospital|