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Age-Related Changes in Myosin Heavy Chain Composition in Human Orbicularis Oculi Muscle

This study has been completed.
Information provided by:
National Taiwan University Hospital Identifier:
First received: September 9, 2005
Last updated: NA
Last verified: October 2003
History: No changes posted
Orbicularis oculi muscle is a key structure in the peri-orbital anatomy and is responsible for the major changes of aging in this area. Peri-orbital rejuvenation has been an issue of great interest in plastic surgery. We believe that understanding the age-related histomorphometric changes in orbicularis oculi muscle is important for improving the current rejuvenation techniques or developing the new ones. Nevertheless, a study focusing on this topic has never been performed before, simply because it is difficult and dangerous to perform needle biopsy on orbicularis oculi muscle.

Condition Intervention Phase
Procedure: obtain surgical specimen for analysis
Phase 1

Study Type: Interventional
Study Design: Allocation: Non-Randomized
Intervention Model: Single Group Assignment
Masking: Open Label
Primary Purpose: Educational/Counseling/Training

Further study details as provided by National Taiwan University Hospital:

Primary Outcome Measures:
  • Differentiation capability of preadipocyte

Secondary Outcome Measures:
  • Proliferation capability of preadipocyte

Estimated Enrollment: 60
Study Start Date: January 2004
Estimated Study Completion Date: August 2004
Detailed Description:

Hundreds of people came to our hospital requesting upper blepharoplasty every year. Taking advantage of the discarded soft tissue in the blepharoplasty surgery, samples of bilateral orbicularis oculi muscle can be easily obtained from patients of various ages. This study is designed to objectively evaluate the age-related changes in human orbicularis oculi muscle by analyzing the composition of isoforms of myosin heavy chain (MHC), which is the predominant protein in skeletal muscle. MHC isoforms exist in different types of muscle fibers, and determines the maximum contraction speed of the muscle fiber. Therefore, The composition of MHC isoforms represents the composition of different types of muscle fiber.

These muscle specimens are stored with liquid nitrogen right after surgery. In the lab, the specimens are sectioned to 40μm in thickness, and soaked in special buffer solution. After electrophoresis and staining, bands of different types of MHC can be visualized. The gel is scanned and processed with an image analysis system. The density of each band can be determined, and the ratio of the different types of MHC can be calculated.

By dividing the subjects who had their orbicularis oculi muscle sampled during upper blepharoplasty operation into age groups of 20, 30, 40, 50, 60 and 70 years, these MHC subtypes composition ratios can be compared among the different groups. Therefore, the age-related changes of the composition of different muscle types can be concluded.


Ages Eligible for Study:   20 Years to 80 Years   (Adult, Senior)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes

Inclusion Criteria:

  • patients receiving blepharoplasty

Exclusion Criteria:

  Contacts and Locations
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Please refer to this study by its identifier: NCT00154453

Sponsors and Collaborators
National Taiwan University Hospital
Principal Investigator: Yueh-Bih Tang, M.D. Ph.D. National Taiwan University Hospital
  More Information Identifier: NCT00154453     History of Changes
Other Study ID Numbers: 9261701166
Study First Received: September 9, 2005
Last Updated: September 9, 2005

Keywords provided by National Taiwan University Hospital:
Orbicularis oculi
Myosin heavy chain (MHC) processed this record on April 25, 2017