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Study the Effect of Oral Zinc Supplementation on Enzymes of Nitric Oxide Pathway

This study has been completed.
Ministry of Health, Iraq
Information provided by (Responsible Party):
mahmoud hussein hadwan, Babylon University Identifier:
First received: September 9, 2012
Last updated: September 11, 2012
Last verified: September 2012
The ability of spermatozoa to produce reactive oxygen species (ROS) has been respected since the 1940's. Oxidative stress limits the functional competence of mammalian spermatozoa via lipid peroxidation, the induction of oxidative DNA damage and the formation of protein adducts. Nitric oxide (NO) is a free radical generated from the oxidation of L-arginine to L-citrulline by reduced nicotinamide adenine dinucleotide phosphate (NADPH)-dependent NO synthases. Several studies suggest that the overproduction of this free radical and the subsequent excessive exposure to oxidative conditions have a potential pathogenetic implication, which due to the reduction of sperm motility. The present study was conducted to study the effect of Zn supplementation on the levels of NO synthase and arginase in semen of patients with asthenozoospmia.

Condition Intervention
Asthenozoospermia Dietary Supplement: zinc sulfate

Study Type: Observational
Study Design: Observational Model: Case Control
Time Perspective: Retrospective
Official Title: Study the Effect of Oral Zinc Supplementation on Enzymes of Nitric Oxide Pathway

Resource links provided by NLM:

Further study details as provided by mahmoud hussein hadwan, Babylon University:

Primary Outcome Measures:
  • Nitric oxide synthase activity [ Time Frame: at the end of three months ]

Secondary Outcome Measures:
  • Arginase activity [ Time Frame: at the end of three months ]

Enrollment: 60
Study Start Date: July 2011
Study Completion Date: July 2012
Primary Completion Date: July 2012 (Final data collection date for primary outcome measure)
Groups/Cohorts Assigned Interventions
Single group
Single group: each participant receive same intervention (zinc sulfate) throughout study (non-randomized)
Dietary Supplement: zinc sulfate
every participant took two capsules of zinc sulfate per day for three months (each one 220mg)

Detailed Description:
Previous studies suggest that high concentrations of NO play an injurious consequence on spermatozoa kinetic characteristics. These studies reported that NO may react with superoxide or hydrogen peroxide, resulting in the production of peroxinitrite, hydroxyl radical, or singlet oxygen, which cause oxidation of sperm membrane lipids and thiol proteins. NO also may inhibit cellular respiration by nitro-sylation of heme in mitochondrial enzymes, aconitase, and glyceraldehyde phosphate dehydrogenase, leading to a reduction of adenosine triphosphate and that is due to loss of motility of spermatozoa.

Ages Eligible for Study:   27 Years to 35 Years   (Adult)
Sexes Eligible for Study:   Male
Accepts Healthy Volunteers:   No
Sampling Method:   Non-Probability Sample
Study Population
this study includes 60 fertile male partners from couples who had consulted the infertility clinic of babil hospital of maternity (Hilla city/ IRAQ).

Inclusion Criteria:

  • the presence of asthenozoospermia in the semen sample.

Exclusion Criteria:

  • the absence of endocrinopathy,
  • varicocele, and
  • female factor infertility. Smokers and alcoholic men were excluded from the study because of their recognized high seminal ROS levels and decreased antioxidant levels
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Please refer to this study by its identifier: NCT01684059

Babylon university/ college of science
Hilla, Iraq, IQ
Sponsors and Collaborators
Babylon University
Ministry of Health, Iraq
Principal Investigator: mahmoud H. hadwan, phD babylon university / Iraq
Study Chair: Lamia A. Almashhedy, phD Babylon university/Iraq
Study Director: abdulrrazaq S. Alsalman, phD Babylon university/Iraq
  More Information

Publications automatically indexed to this study by Identifier (NCT Number):
Responsible Party: mahmoud hussein hadwan, phD, Babylon University Identifier: NCT01684059     History of Changes
Other Study ID Numbers: Babil-2
Study First Received: September 9, 2012
Last Updated: September 11, 2012

Additional relevant MeSH terms:
Infertility, Male
Genital Diseases, Male
Nitric Oxide
Zinc Sulfate
Bronchodilator Agents
Autonomic Agents
Peripheral Nervous System Agents
Physiological Effects of Drugs
Anti-Asthmatic Agents
Respiratory System Agents
Free Radical Scavengers
Molecular Mechanisms of Pharmacological Action
Neurotransmitter Agents
Endothelium-Dependent Relaxing Factors
Vasodilator Agents
Protective Agents
Trace Elements
Growth Substances
Dermatologic Agents processed this record on June 23, 2017