Effect of PCR-CRISPR/Cas12a on the Early Anti-infective Schemes in Patients With Open Air Pneumonia
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|ClinicalTrials.gov Identifier: NCT04178382|
Recruitment Status : Recruiting
First Posted : November 26, 2019
Last Update Posted : November 26, 2019
|Condition or disease||Intervention/treatment||Phase|
|Severe Sepsis||Diagnostic Test: PCR-CRISPR／Cas12a detection||Not Applicable|
|Study Type :||Interventional (Clinical Trial)|
|Estimated Enrollment :||146 participants|
|Intervention Model:||Parallel Assignment|
|Masking:||Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)|
|Official Title:||Effect of PCR-CRISPR/Cas12a on the Early Anti-infective Schemes in Patients With Open Air Pneumonia|
|Actual Study Start Date :||August 1, 2019|
|Estimated Primary Completion Date :||July 30, 2020|
|Estimated Study Completion Date :||August 30, 2020|
Experimental: experiment group
Combined detection of PCR and CRISPR/Cas12a in alveolar lavage fluid to guide early target adjustment of antibiotics
Diagnostic Test: PCR-CRISPR／Cas12a detection
Evaluate whether the combination of PCR and CRISPR/Cas12a detection of alveolar lavage fluid changes the choice of early antibiotics in patients with pneumonia in artificial airways, and whether it changes the prognosis compared with traditional pathogenic microbial detection techniques.
No Intervention: control group
Guide the target adjustment of antibiotics according to traditional microbiological detection methods
- mortality [ Time Frame: up to 28 days after hospitalized. ]The patient's 28-day mortality rate is the survival rate from the onset to the disease at 28 days, compared with the total number of illnesses, to assess the severity of the disease.
- the duration of intensive care unit [ Time Frame: Up to 8 weeks ]time for patients treatment in intensive care unit is patient total treated days in intensive care unit.
- the length of hospital stay [ Time Frame: Up to 8 weeks ]time for patients treatment in hospital is patient total treated days in hosiptal. Index of treat effective and severity of disease.
- the day of mechanical ventilation [ Time Frame: Up to 8 weeks ]time for patients need mechanical ventilation is the total days that patient need mechanical ventilation.
- the duration of septic shock [ Time Frame: up to 8 weeks ]The more severity patients had a long time during septic shock
- the incidence of antibiotic-associated diarrhea [ Time Frame: up to 8 weeks ]The incidence of antibiotic-associated diarrhea is the index of side effects of anti-infective treatment. The time frame started after the use of antibiotics, during the total hospitalized.
- the incidence of new multi-drug resistant bacteria colonization or infection [ Time Frame: up to 8 weeks. ]rate of multi-drug resistant bacteria colonization or infection is the index of side effects of anti-infective treatment. The time frame started after the use of antibiotics, during the total hospitalized.
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT04178382
|Contact: yan wang, MDemail@example.com|
|The Affliated Drum Tower Hospital, Medical School of Nanjing University||Recruiting|
|Nanjing, Jiangsu, China, 210008|
|Contact: Wenkui Yu 02568182222 ext 60506 firstname.lastname@example.org|
|Study Chair:||kui w yu, phd||The Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical School|