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To Identify HLA-A1101-restricted Peptide Epitopes Derived From Novel Oncoantigens (URLC10, KIF20A, and CDCA1) Applicable for Cancer Vaccine in Singapore

The recruitment status of this study is unknown. The completion date has passed and the status has not been verified in more than two years.
Verified December 2013 by National University Hospital, Singapore.
Recruitment status was:  Recruiting
Information provided by (Responsible Party):
National University Hospital, Singapore Identifier:
First received: April 2, 2012
Last updated: December 10, 2013
Last verified: December 2013

Aim: To identify HLA-A1101-restricted peptide epitopes derived from novel Oncoantigens (URLC10, KIF20A, and CDCA1) applicable for Cancer Vaccine in Singapore.

Methods: The panel of candidate peptides are synthesized and tested for their ability to induce peptide-specific CTL responses, in order to screen the peptide epitopes applicable for the cancer vaccination. Briefly, peripheral blood lymphocytes (PBLs) derived from HLA-A1101(+) healthy donors are taken and cultured in the presence of the each candidate peptide with recombinant IL-2 for 2 weeks, and then, re-stimulated with dendritic cell pulsed with the peptide following another 2 week culture. Thereafter, CD8(+) T lymphocytes were negatively selected with CD4-magnetic beads from cultured lymphocytes and tested for their peptide specificity employing enzyme-linked immunospot (ELISPOT) assay. These conditions are completely performed in in-vitro system. Importance in medicine: If one could identify the peptide epitopes from novel Oncoantigens, it is applicable for clinical trials of cancer vaccination.

Benefits & Risks : There is no risk except for the matter of venipuncture in each individuals.

The ideal target molecules for cancer vaccination are thought to be selectively expressed in tumor cells, but not in the normal cells, with high frequent and homogenous expression within tumor. We have proved that novel Oncoantigens, URLC10, KIF20A and CDCA1, have these characters as ideal target molecules for the cancer vaccination and are highly expressed in a variety of tumor type such as gastric, lung, and pancreas cancer. Since HLA-A1101 haplotype is most frequent in Singaporean, it is essential to indentify the HLA-A1101-restriced peptides derived from these Oncoantigens to develop cancer vaccination.

Anonymous Donors at Blood Donation Center (NUH)

Study Type: Observational
Study Design: Observational Model: Cohort
Time Perspective: Prospective

Further study details as provided by National University Hospital, Singapore:

Primary Outcome Measures:
  • To identify HLA-A1101-restricted peptide epitopes derived from novel Oncoantigens (URLC10, KIF20A, and CDCA1) applicable for Cancer Vaccine in Singapore.

Estimated Enrollment: 10
Study Start Date: January 2012
Estimated Primary Completion Date: December 2014 (Final data collection date for primary outcome measure)

Ages Eligible for Study:   22 Years to 80 Years   (Adult, Senior)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes
Sampling Method:   Probability Sample
Study Population
Anonymous donors at blood donation center in NUH.

Inclusion Criteria:

  • The participants should be healthy,
  • non-pregnant adults who weigh at least 50kgs

Exclusion Criteria:

  • Age of < 22 and > 80 years old.
  • Pregnancy (women of childbearing potential: Refusal or inability to use effective means of contraception)
  • Decision of unsuitableness by Principal Investigator or physician-in-charge.
  Contacts and Locations
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Please refer to this study by its identifier: NCT01570439

Contact: Wei Peng Yong, MRCP +65 6779 5555

Nationa University Hospital Recruiting
Singapore, Singapore
Contact: Wei Peng Yong, MRCP    +65 6779 5555   
Principal Investigator: Wei Peng Yong, MRCP         
Sponsors and Collaborators
National University Hospital, Singapore