Study of Methicillin-resistant Staphylococcus Aureus (MRSA) Isolated From Infected Patients in Brazil (MRSABrazil)

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT01359358
Recruitment Status : Unknown
Verified May 2011 by University of Sao Paulo.
Recruitment status was:  Not yet recruiting
First Posted : May 24, 2011
Last Update Posted : May 24, 2011
Information provided by:
University of Sao Paulo

Brief Summary:

This project has as general objective to determine if there is any Methicillin-resistant Staphylococcus aureus (MRSA) strain disseminating in the hospital of Belo Horizonte. If this is the case, the investigators will determine if this is an international known strain by typing it by PFGE and MLST.

The detection of mecA gene will be performed by PCR. Their susceptibility profile to several drugs will also be accessed and it will be possible to compare the response to those drugs commonly in use to that of Daptomycin, a new drug available in Brazil.

It will be screened for reduced susceptibility to vancomycin by Macromethod Etest (MET) in order to search for VISA or hetero-VISA. Also, due to the discussions at the literature about mutations in genes that are said to be responsible for reduced susceptibility to vancomycin, the investigators will sequence these genes in all PFGE type strain of this study looking for mutations already described to compare to the screening results.

Condition or disease
Methicillin Resistant Staphylococcus Aureus

Detailed Description:
Multidrug resistant and virulent MRSA strains have been disseminating worldwide and few alternatives of treatment for diseases caused by this pathogen are available. In this study, 36 isolates from colonization or different clinical specimens from infected patients of the Hospital Rizoleta Tolentino Neves will be analyzed. These results will provide important data for doctors and nurses, such as whether there is any strain dissemination or not in the hospital what could suggest an outbreak. It will be performed molecular typing by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The investigators will evaluate the efficiency of several antimicrobials and the frequency of h-VISA and VISA among the Brazilian MRSA. Besides, the investigators will verify the presence of mutation in genes that are involved in resistance mechanisms of VISA. According to the strains profile present in the hospital, if this is suggested that there is a dissemination of some strain, the health professionals can start a more rigid infection control measures in order to decrease the number of cases of nosocomial infection, decreasing the time of hospitalization of patients and, consequently the cost for the hospital.

Study Type : Observational
Estimated Enrollment : 20 participants
Time Perspective: Retrospective
Official Title: Epidemiological Study of Methicillin-resistant Staphylococcus Aureus (MRSA) Isolated From Infected Patients During a Hospital Surveillance Period
Study Start Date : July 2011

Resource links provided by the National Library of Medicine

MedlinePlus related topics: MRSA

Information from the National Library of Medicine

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Ages Eligible for Study:   1 Month and older   (Child, Adult, Older Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   No
Sampling Method:   Non-Probability Sample
Study Population
MRSA from infection or from colonization of a patient who was already infected by MRSA during the time of the study

Inclusion Criteria:

  • MRSA from infection sites
  • MRSA from colonization of the patients who already was infected by MRSA during the time of the study

Exclusion Criteria:

  • MRSA from colonization of a patient who has not infection caused by MRSA

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT01359358

Contact: Hyllo Baeta MArcelo Jr, Master degree 55 31 99929631

Instituto de Física de Sao Carlos - Universidade de Sao Paulo Active, not recruiting
Sao Carlos, Sao Paulo, Brazil, 13560-590
Sponsors and Collaborators
University of Sao Paulo
Principal Investigator: Ilana L B Camargo, PhD University of Sao Paulo

Responsible Party: Ilana Lopes Baratella da Cunha Camargo, University of Sao Paulo Identifier: NCT01359358     History of Changes
Other Study ID Numbers: Novartis IFSC-USP
First Posted: May 24, 2011    Key Record Dates
Last Update Posted: May 24, 2011
Last Verified: May 2011

Keywords provided by University of Sao Paulo:
isolated from colonization

Additional relevant MeSH terms:
Staphylococcal Infections
Gram-Positive Bacterial Infections
Bacterial Infections
Anti-Bacterial Agents
Anti-Infective Agents