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Expression Levels of RISC and Microprocessor Complex Components in Epithelial Skin Cancer

This study has been completed.
Sponsor:
ClinicalTrials.gov Identifier:
NCT01345760
First Posted: May 2, 2011
Last Update Posted: October 16, 2012
The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details.
Information provided by (Responsible Party):
Michael Sand, Ruhr University of Bochum
  Purpose
MicroRNAs (miRNAs) are very small endogenous RNA molecules about 22-25 nucleotides in length, capable of post-transcriptional gene regulation. miRNAs bind to their target messenger RNAs (mRNAs), leading to cleavage or suppression of target mRNA translation based on the degree of complementarity. miRNAs have recently been shown to play pivotal roles in diverse developmental and cellular processes and linked to a variety of skin diseases and cancers. In the present study, the investigators examines the expression profiles of the microprocessor complex subunit DGCR8 and the RISC components TARBP1, TARBP2, argonaute-1, argonaute-2 and PACT in epithelial skin cancer and its premalignant stage.

Condition
Skin Cancer

Study Type: Observational
Study Design: Observational Model: Case-Only
Time Perspective: Retrospective
Official Title: Expression Levels of RNA-induced Silencing Complex (RISC) Components TARBP1, TARBP2, Argonaute-1, Argonaute-2, PACT and Microprocessor Complex Component DGCR8 in Epithelial Skin Cancer

Resource links provided by NLM:


Further study details as provided by Michael Sand, Ruhr University of Bochum:

Biospecimen Retention:   Samples With DNA
human skin

Enrollment: 44
Study Start Date: July 2008
Study Completion Date: February 2011
Primary Completion Date: February 2011 (Final data collection date for primary outcome measure)
Groups/Cohorts
Basal Cell Carcinoma
Squamous Cell Carcinoma
Actinic Keratosis
healthy non-lesional skin

Detailed Description:
Patients with premalignant actinic keratoses, basal cell carcinomas and squamous cell carcinomas were included in the study. Punch biopsies were harvested from the center of the tumors, from sites of healthy skin and a healthy control group. DGCR8, TARBP1, TARBP2, argonaute-1, argonaute-2 and PACT mRNA expression levels were detected by quantitative real-time reverse transcriptase polymerase chain reaction.
  Eligibility

Information from the National Library of Medicine

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Ages Eligible for Study:   18 Years and older   (Adult, Senior)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes
Sampling Method:   Non-Probability Sample
Study Population
Patients with epithelial skin cancer and pre-malignant stages
Criteria

Inclusion Criteria:

  • epithelial skin cancer

Exclusion Criteria:

  • other known previous malignancies
  Contacts and Locations
Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT01345760


Locations
Germany
Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum
Bochum, NRW, Germany, 44791
Sponsors and Collaborators
Ruhr University of Bochum
Investigators
Study Director: Falk G Bechara, PD Dr. Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum
  More Information

Publications:
Responsible Party: Michael Sand, Dr., Ruhr University of Bochum
ClinicalTrials.gov Identifier: NCT01345760     History of Changes
Other Study ID Numbers: 1-Sand
First Submitted: April 29, 2011
First Posted: May 2, 2011
Last Update Posted: October 16, 2012
Last Verified: October 2012

Keywords provided by Michael Sand, Ruhr University of Bochum:
Basal Cell Carcinoma
Squamous Cell Carcinoma
Actinic Keratosis
microRNA
Dicer
Drosha
RISC
microprocessor complex
Argonaute
DGCR8
TARBP

Additional relevant MeSH terms:
Skin Neoplasms
Neoplasms by Site
Neoplasms
Skin Diseases