Functional Role of RUNX1 Mutations in the Etiology of Acute Myeloid Leukemia (AML)
|ClinicalTrials.gov Identifier: NCT01329471|
Recruitment Status : Unknown
Verified April 2011 by Hillel Yaffe Medical Center.
Recruitment status was: Not yet recruiting
First Posted : April 6, 2011
Last Update Posted : April 6, 2011
|Condition or disease|
|Acute Myeloid Leukemia|
The RUNX1 gene, located at chromosomal band 21q22, is a transcription factor, crucial for hematopoiesis and the generation of hematopoietic stem cells in the embryo. RUNX1 is the most frequent target for chromosomal translocation in leukemia. In addition, point mutations in the RUNX1 gene have been found to constitute an important mode of genetic alteration in development of leukemia. Recent publications stressing the clinical need for implementing RUNX1 point mutations as both a diagnostic and unfavorable prognostic marker of AML, have aroused particular interest in the functional role of RUNX1 in this disease.
In order to pinpoint specific RUNX1 target genes involved in pre-leukemic transformation or exacerbation of existing leukemia, the investigators plan to compare expression profiles from human hematopoietic progenitors overexpressing a mutated form of RUNX1with controls (RUNX1 wild-type and knocked-down). In this study the investigators intend to collect blood, after receiving informed consent, from umbilical cords of neonates born vaginally, in order to isolate CD34+ hematopoietic progenitors. Human umbilical cord blood contains relatively high numbers of CD34+ cells, which may be frozen directly after collection and used as a source of progenitor cells for further culture or direct analysis.
|Study Type :||Observational|
|Estimated Enrollment :||75 participants|
|Study Start Date :||April 2011|
|Estimated Primary Completion Date :||April 2012|
|Estimated Study Completion Date :||April 2013|
|Umbilical cord blood|
- Performance of expression arrays on transfected CD34+ cells [ Time Frame: One year ]Performance of expression arrays on transfected CD34+ cells (derived from human cord blood), expecting differential gene expression between the wild type RUNX1-transfected cells and mutated RUNX1-transfected cells.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT01329471
|Hillel Yaffe Medical Center||Not yet recruiting|
|Hadera, Israel, 38100|
|Contact: Ofer Fainaru, MD, PhD firstname.lastname@example.org|