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Tissue Sectioning by Electro-Dissociation

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT01054612
Recruitment Status : Withdrawn (The study was closed due to the departure of several of the co-investigators.)
First Posted : January 22, 2010
Last Update Posted : March 4, 2015
Information provided by:
University of Arkansas

Brief Summary:
Currently there is no technique to produce thin (0.004-0.01 mm) serial sections of large fresh tissue specimens that are suitable for high-resolution in situ protein/gene expression studies without ice artifact or fixation-induced molecular damage. Traditional frozen sectioning preserves protein and nucleic acid structure, but the inherent ice artifact precludes reconstruction of protein and mRNA expression patterns in 3-dimensions. Since the limitations of the existing sectioning techniques result from the fact that they rely on mechanical cutting which in turn require the tissue to be stiff, we suggest a new approach to cut tissue via an electro erosion process that utilizes focus radio frequency (RF).

Condition or disease Intervention/treatment
Thin Sections Fresh Unfixed Tissues Fresh Unfrozen Tissues Other: Tissue Sectioning via Electro Erosion Process

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Study Type : Observational
Actual Enrollment : 0 participants
Official Title: Tissue Sectioning by Electro-Dissociation
Study Start Date : March 2002
Actual Primary Completion Date : January 2010
Actual Study Completion Date : January 2010

Resource links provided by the National Library of Medicine

Intervention Details:
  • Other: Tissue Sectioning via Electro Erosion Process
    Focused RF energy can be used to produce consecutive thin sections of fresh tissue for immunohistochemical and nucleic acids analysis by electro-dissociation without thermal damage, ultimately allowing high resolution reconstruction of gene and protein expression patterns of large tissue specimens in 3D.

Information from the National Library of Medicine

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Ages Eligible for Study:   Child, Adult, Older Adult
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   No
Sampling Method:   Probability Sample
Study Population
Discarded human tissue obtained immediately following surgical resection

Inclusion Criteria:

  • Fresh Tissue
  • Unfrozen Tissue
  • Unfixed Tissue

Exclusion Criteria:

  • Unfresh Tissue
  • Frozen Tissue
  • Fixed Tissue

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT01054612

Sponsors and Collaborators
University of Arkansas
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Principal Investigator: Gal Shafirstein, Ph.D. UAMS, ACH, Central Arkansas Veterans Healthcare System

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Responsible Party: Dr. Gal Shafirstein, University of Arkansas, Arkansas Children's Hospital Identifier: NCT01054612     History of Changes
Other Study ID Numbers: IRB 28177
First Posted: January 22, 2010    Key Record Dates
Last Update Posted: March 4, 2015
Last Verified: March 2015
Keywords provided by University of Arkansas:
Gene Expression
Tissue Sectioning
Additional relevant MeSH terms:
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Dissociative Disorders
Mental Disorders