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Using Heavy Water to Study Cell Dynamics in Parkinson's Disease

The recruitment status of this study is unknown. The completion date has passed and the status has not been verified in more than two years.
Verified March 2015 by KineMed.
Recruitment status was:  Active, not recruiting
Michael J. Fox Foundation for Parkinson's Research
Information provided by (Responsible Party):
Salena Killion, KineMed Identifier:
First received: October 2, 2009
Last updated: March 30, 2015
Last verified: March 2015

This pilot study will assess the feasibility of using heavy water as a safe 'tracer' for biomarker studies of diseases of the brain and spinal cord, that, together, are also called the central nervous system (CNS). Heavy water, also called deuterated water or D20, is the same as normal drinking water except the hydrogen atoms have been replaced by deuterium, a naturally occurring isotope of hydrogen. In particular, this study will use heavy water to define: 1) The rate of immune cell proliferation (growth) in the cerebrospinal fluid (CSF) compared to blood. This study will be examining a particular type of immune cell called T lymphocytes. 2) This study will also examine selected molecules generated by nerve cells of the CNS to understand their rate of secretion and turnover in healthy control participants, HIV-1-infected participants and participants with a non-HIV-related neurodegenerative disease such as Parkinson's disease (PD).

This study will involve the administration of heavy water orally for either seven days, 12 days or six weeks. Measurements will be taken by lumbar puncture (LP, also known as a spinal tap). Blood (approximately five tablespoons per visit) will also be obtained at each of the lumbar puncture appointments.

If this method can be used to establish the rates of immune cell turnover and the production rates of neuronal molecules using cerebrospinal fluid, it will provide unique data that is important to understand chronic neurodegenerative conditions, like PD, and to measure responses to targeted therapies.


  1. D2O, administered orally, can be used to measure the proliferation rates of CSF T cells (and, eventually, of their major phenotypic subsets).
  2. D2O can be used to assess the turnover and production rates of CNS constituents that are normally or pathologically shed or secreted into the CSF, including (eventually): cargo molecules transported specifically in neurons in the CNS, such as chromogranin-A and -B, neuregulin-1 (specifically the extracellular secreted ectodomain of neuronal differentiation factor (NDF) isoform type α1, α2, β1, and the acetylcholine receptor inducing activity isoform (ARIA), secreted amyloid precursor protein (sAPP), alpha-synuclein; and APP metabolites amyloid beta (Aβ) 41 and 42.

Parkinson's Disease
HIV Infections

Study Type: Observational
Study Design: Observational Model: Cohort
Official Title: Cellular and Molecular Kinetics of Cerebrospinal Fluid (CSF) Using Heavy Water Labeling Method: A Study of Healthy Controls, CNS HIV Infection, Parkinson's Disease and Other Neurodegenerative Diseases

Resource links provided by NLM:

Further study details as provided by KineMed:

Primary Outcome Measures:
  • CSF Biomarkers [ Time Frame: 40 days ]

Biospecimen Retention:   Samples Without DNA

Estimated Enrollment: 45
Study Start Date: April 2009
Estimated Study Completion Date: February 2016
Estimated Primary Completion Date: February 2016 (Final data collection date for primary outcome measure)
Healthy men and women, 18 years of age or older, who have no history of significant medical conditions.
HIV positive
Men and women, 18 years of age or older, who have been diagnosed with HIV infection. Patients may be on or off of ARVs.
Parkinson's Disease
Men and women, 18 years of age or older, who have been diagnosed with Parkinson's Disease.


Ages Eligible for Study:   18 Years and older   (Adult, Senior)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes
Sampling Method:   Non-Probability Sample
Study Population

Healthy controls, HIV positive (on or off ARVs), patients diagnosed with Parkinson's Disease.

All subjects must be 18 years of age or older.


Inclusion Criteria:

  • 18 years of age or older
  • healthy controls with no significant medical conditions
  • diagnosed HIV positive patients on or off ARVs
  • diagnosed Parkinson's Disease patients
  • capacity to provide informed consent

Exclusion Criteria:

  Contacts and Locations
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Please refer to this study by its identifier: NCT00990379

United States, California
San Francisco General Hospital
San Francisco, California, United States, 94110
Sponsors and Collaborators
Salena Killion
Michael J. Fox Foundation for Parkinson's Research
Principal Investigator: Richard Price, MD University of California, San Francisco
  More Information

Responsible Party: Salena Killion, Director of Clinical Studies, KineMed Identifier: NCT00990379     History of Changes
Other Study ID Numbers: 6076
Study First Received: October 2, 2009
Last Updated: March 30, 2015

Keywords provided by KineMed:
brain diseases
spinal fluid
HIV positive

Additional relevant MeSH terms:
Parkinson Disease
HIV Infections
Acquired Immunodeficiency Syndrome
Neurodegenerative Diseases
Parkinsonian Disorders
Basal Ganglia Diseases
Brain Diseases
Central Nervous System Diseases
Nervous System Diseases
Movement Disorders
Lentivirus Infections
Retroviridae Infections
RNA Virus Infections
Virus Diseases
Sexually Transmitted Diseases, Viral
Sexually Transmitted Diseases
Immunologic Deficiency Syndromes
Immune System Diseases
Slow Virus Diseases
Deuterium Oxide
Antineoplastic Agents
Radiation-Protective Agents
Protective Agents
Physiological Effects of Drugs processed this record on April 24, 2017