Release of Cytokines by Stimulated Peripheral Blood Mononuclear Cells (PBMC) From Chronic Periodontitis Subjects

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT00990041
Recruitment Status : Completed
First Posted : October 6, 2009
Last Update Posted : October 6, 2009
Information provided by:
Escola Bahiana de Medicina e Saude Publica

Brief Summary:

Background and Objective: It has been reported that peripheral blood mononuclear cells (PBMC) from periodontal patients have a different profile of cytokine release when compared to the ones of healthy patients. Cigarette smoking is a recognized risk factor for periodontitis and is known to affect the systemic and local immune responses. Thus, the aim of the present study was to obtain preliminary data about the interaction of periodontal condition and smoking in the secretion of some cytokines by PBMC.

Material and Methods: PBMC samples were isolated from 19 donors, divided into generalized chronic periodontitis (P; n=10) and periodontal health (H; n=9) groups. In addition, the smokers and non-smokers of each group were separately evaluated: SP- smokers with severe generalized chronic periodontitis (n=5); SH- periodontally healthy smokers (n=5); NSP- non-smokers with severe generalized chronic periodontitis (n=5) and NSH- periodontally healthy non-smokers (n=4). Cells were incubated for 24-48 hours in 500 µL wells containing RPMI 1640 and stimulated with 1.0 ng/mL of E. coli LPS. Supernatants were used to quantify the amounts of TNF-α, IL-6, IL-8 and IL-10 released using ELISA kits.

Condition or disease

Study Type : Observational
Observational Model: Case Control
Time Perspective: Cross-Sectional


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Ages Eligible for Study:   25 Years to 55 Years   (Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes
Sampling Method:   Non-Probability Sample
Study Population
healthy humans

Inclusion Criteria:

  • periodontitis

Exclusion Criteria:

  • diabetes

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT00990041

Faculdade de Odontologia de Piracicaba
Piracicaba, SP, Brazil, 13000
Sponsors and Collaborators
Escola Bahiana de Medicina e Saude Publica
FOP-UNICAMP Identifier: NCT00990041     History of Changes
Other Study ID Numbers: 9002-10-04.PBMC
First Posted: October 6, 2009    Key Record Dates
Last Update Posted: October 6, 2009
Last Verified: October 2009

Keywords provided by Escola Bahiana de Medicina e Saude Publica:
find cytokines in blood of periodontal patients

Additional relevant MeSH terms:
Chronic Periodontitis
Periodontal Diseases
Mouth Diseases
Stomatognathic Diseases