Differential Regulation of VEGF, Cadherin, Angiopietin 2 by Trigger Oocyte Maturation With GnRHa vs hCG in Donors
|Vascular Endothelial Growth Factor Overexpression|
|Study Design:||Observational Model: Cohort
Time Perspective: Prospective
|Official Title:||Differential Regulation of VEGF, Cadherin and Angiopietin 2 by Trigger Oocyte Maturation With GnRHa vs hCG in Donors: Try to Explain the Lower OHSS Incidence|
- VEGF Protein and mRNA Levels [ Time Frame: 1 year ]VEGF concentration in follicular fluid and VEGF mRNA expression in granulosa cells from patients who received either GnRH agonist instead of hCG
Biospecimen Retention: Samples With DNA
|Study Start Date:||June 2008|
|Study Completion Date:||March 2009|
|Primary Completion Date:||February 2009 (Final data collection date for primary outcome measure)|
Antagonist-hCG for triggering
Protocol with antagonist and hCG to trigger oocyte maturation
Antagonist-aGnRH for triggering
Protocol with antagonist and 0,2 mg triptorelin to trigger oocyte maturation
Long protocol-hCG for triggering
Long Protocol and hCG to trigger oocyte maturation
Material and Methods Between June and December of 2008 we evaluated 90 egg donors. They underwent COH with 150 IU rFSH as starting dose, we separated them in 3 groups (n:30 each): Groups 1 and 2 received a 0.25mg daily dose of GnRH antagonist when a follicle 14mm in diameter was observed. hCG 250g was given to group 1 while group 2 received triptorelin 0.2mg when leading two follicles were 17mm. Group 3 was stimulated with standard long protocol, hCG was given following similar criteria.
Blood was collected the day of hCG/aGnRH administration as well as the day of egg retrieval, and follicular fluid from the first two mature follicles was also frozen. We collected granulosa cells (GC) of 10 patients of each group as well Levels of VEGF, sVE-Cadherin an Angiopietin 2 were determined by ELISA in serum and in follicular fluid.
Results were analyzed via ANOVA.Data are expressed as mean± SEM. A significant difference was defined as p< 0.05.