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Stem Cell Study for Patients With Leg Ulcer/Gangrene

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT00221143
Recruitment Status : Completed
First Posted : September 22, 2005
Last Update Posted : February 9, 2009
Institute of Biomedical Research and Innovation, Kobe, Hyogo, Japan
Kobe City General Hospital
Information provided by:
Translational Research Center for Medical Innovation, Kobe, Hyogo, Japan

Brief Summary:
The purpose of this study is to determine if stem cell therapy with one's own cells (autologous cells) delivered intramuscularly to one's leg with ulcer and/or gangrene due to poor blood flow will be safe and if it will relieve leg pain, increase blood flow, and/or cure the leg wound.

Condition or disease Intervention/treatment Phase
Leg Pain Ulcer Gangrene Ischemia Peripheral Vascular Diseases Genetic: Autologous peripheral blood CD34 positive cell therapy Phase 1 Phase 2

Detailed Description:
Chronic critical limb ischemia (CLI) is a progressive disease, which arises as a result of atherosclerosis or vasculitis in leg arteries. Prognosis of chronic CLI is poor, and no effective treatments have been established in patients who are not eligible for the traditional revascularization therapies such as angioplasty and bypass procedures due to the inappropriate anatomy of the leg arteries or frequent reocclusion following revascularization. Therefore, it is necessary to establish novel revascularization treatment to improve prognosis of the no-option patients. We will study the safety and clinical efficiency of vascular regeneration by means of transplantation of autologous peripheral blood endothelial progenitor cells (CD34 positive cells) in patients with chronic CLI who are not eligible for traditional revascularization treatments. The primary endpoint is the primary efficacy score identified by toe brachial blood pressure index (TBPI), absolute claudication distance (ACD) and Wong Baker's pain rating scale, while the secondary endpoints are evaluation of safety, ankle brachial blood pressure index (ABPI), percutaneous tissue oxygen pressure (TcPO2), etc.

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Study Type : Interventional  (Clinical Trial)
Actual Enrollment : 15 participants
Allocation: Non-Randomized
Intervention Model: Single Group Assignment
Masking: None (Open Label)
Primary Purpose: Treatment
Official Title: Phase I / II Clinical Trial Regarding Vascular Regeneration Therapy by Transplantation of Autologous Peripheral Blood Endothelial Progenitor Cells (CD34+ Cells) in No-Option Patients With Chronic Critical Limb Ischemia
Study Start Date : November 2003
Actual Study Completion Date : January 2008

Resource links provided by the National Library of Medicine

MedlinePlus related topics: Gangrene

Primary Outcome Measures :
  1. Major amputation

Secondary Outcome Measures :
  1. Limb ischemia

Information from the National Library of Medicine

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Ages Eligible for Study:   20 Years to 80 Years   (Adult, Older Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   No

Inclusion Criteria:

  • Chronic severe CLI patients fulfilling all the following criteria are considered suitable for inclusion in the study.

    1. At least 6 months since the onset of CLI (Chronic peripheral artery disease or Buerger disease)
    2. Patients with luminal stenosis > 50% by leg angiography
    3. Age is between 20 and 80.
    4. Patients whose Rutherford's class is II-4, III-5, or III-6(Patients with rest pain or ischemic ulcer/necrosis)
    5. Patients for whom angioplasty and bypass surgery are not indicated because of anatomical or procedural reasons or frequent reocclusion/ restenosis following the traditional revascularization (No option patients)
    6. Patients who can give informed consent themselves in writing.

Exclusion Criteria:

  • Any one of the following exclusion criteria is sufficient to disqualify a patient from the study.

    1. Left ventricular ejection fraction < 25%
    2. Patients with a history of severe allergic reactions or side effects to G-CSF preparations or apheresis.
    3. Less than 6 months since last episode of myocardial/cerebral infarction.
    4. Patients with unstable angina, with a treatment rating of 3 in the Braunwald system, but a severity of III and a clinical rating of B or C.
    5. Patients with diabetic proliferating retinopathy (new Fukuda classification BI to BV).
    6. Patients with malignant tumor
    7. Patients with chronic rheumatoid arthritis.
    8. Patients with hematological disease (leukemia, myeloproliferative disease, or myelodysplastic syndromes).
    9. Patients currently suffering from or having a history of interstitial pneumonitis.
    10. Patients for whom cranial MRA reveals cerebral aneurysm.
    11. Patients for whom abdominal CT or ultrasonography reveals splenomegaly.
    12. Patients with cirrhosis of the liver.
    13. Patients who cannot discontinue Warfarin.
    14. Leukocytes less than 4,000/µL or exceeding 10,000/µL.
    15. Platelets less than 100,000/µL.
    16. Hemoglobin less than 10 g/dL.
    17. AST (GOT) exceeding 100 IU/L or ALT (GPT) exceeding 100 IU/L.
    18. Patients with severe neural disorder in their legs.
    19. Patients with gait disturbance for reasons other than CLI (such as sciatic neuralgia, or vasculitis), making exercise tolerance evaluation on a treadmill with stress ECG difficult.
    20. Pregnant or nursing patients, those who may be pregnant, or those who plan on becoming pregnant before the end of the study period.
    21. Any other reason that the Clinical Supervisors or Clinical Researchers may have for considering a case unsuitable for the study.

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT00221143

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Kobe City General Hospital
Kobe, Hyogo, Japan, 650-0046
Institute of Biomedical Research and Innovation
Kobe, Hyogo, Japan, 650-0047
Sponsors and Collaborators
Translational Research Center for Medical Innovation, Kobe, Hyogo, Japan
Institute of Biomedical Research and Innovation, Kobe, Hyogo, Japan
Kobe City General Hospital
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Principal Investigator: Takayuki Asahara, M.D. Institute of Biomedical Research and Innovation, Kobe, Hyogo, Japan
Additional Information:
Publications automatically indexed to this study by Identifier (NCT Number):
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Responsible Party: Atsuhiko Kawamoto, MD, PhD Senior Research Scientist, Stem Cell Translational research Team, Institute of Biomedical Research and Innovation Identifier: NCT00221143    
Other Study ID Numbers: BRI ASO 03-01
First Posted: September 22, 2005    Key Record Dates
Last Update Posted: February 9, 2009
Last Verified: February 2009
Keywords provided by Translational Research Center for Medical Innovation, Kobe, Hyogo, Japan:
Ulcer/ gangrene
Leg diseases
Vascular diseases
Chronic limb ischemia
Peripheral artery disease
Additional relevant MeSH terms:
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Vascular Diseases
Peripheral Vascular Diseases
Peripheral Arterial Disease
Pathologic Processes
Cardiovascular Diseases
Arterial Occlusive Diseases