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HIIT and MICT on Nitric Oxide-mediated Erythrocyte Rheology

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ClinicalTrials.gov Identifier: NCT04823429
Recruitment Status : Recruiting
First Posted : March 30, 2021
Last Update Posted : March 30, 2021
Sponsor:
Information provided by (Responsible Party):
Jong-Shyan Wang, Chang Gung Memorial Hospital

Study Description
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Brief Summary:
Erythrocyte rheological properties affect blood viscoelasticity and consequently regulate vascular resistance to flow shear force, whereas rheological impairments of erythrocytes may result in circulatory disorders. The aim of this study was to establish an effective exercise strategy for improving individual aerobic capacity and for simultaneously ameliorating the risk of hemorheological dysfunction evoked by a graded exercise test (GXT) and the hypotheses is exercise intervention will improved hemorheological functions by enhancing deformability of erythrocytes via NO-mediated mechanism. This study included 60 healthy sedentary mens (age 20~30) from Chang Gung university than were randomized into the HIIT [3-min intervals at 40% and 80% V̇O2 reserve (V̇O2R),n=10] and MICT(sustained 60% V̇O2R,n=10)on a bicycle ergometer for 30min·d-1, 5 d·wk-1 for 6 wk.

Condition or disease Intervention/treatment Phase
Exercise Interval Training Behavioral: High intensity-interval training (HIIT) Behavioral: Moderate intensity-continuous (MICT) Not Applicable

Detailed Description:
Recently, the role of erythrocyte has been more emphasized, which also related with endothelial cell. For coronary artery patients, the endothelial nitric oxide synthase activity in red blood cell (RBC-eNOS activity) is lower than age-matched health people, and it is related with dysfunction of endothelial cell. In cardiovascular diseases. the erythrocyte arginase-1 is active and seize L-arginine with eNOS. When the Arg-1 stimulated by reactive oxygen species (ROS), the nitric oxide (NO) bioactivity decrease and produce more ROS, meanwhile, ROS can go around to stimulate Arg-1. When the RBC-NO production is lowering, it will increase the adhesion activity to endothelial cell due to erythrocyte can be quite close to blood vessel well then release Nitric Oxide, induce the dysfunction and oxidative pressure of endothelial cell. The NO can also regulate the deformability of erythrocytes, and extremely affect oxygen supply to tissue once the deformability and aggregation of erythrocyte become abnormal. Besides NO, the deformability will be affected if erythrocyte is continuously exposed to the endogenous or exogenous ROS, which also increase adhesion to endothelial cell with the exposure of phosphatidylserine. Exercise can regulate the mechanism of NO release from erythrocyte, affecting the rheology of erythrocyte, and improve the anti-oxidation ability. Therefore, as mentioned above which make erythrocyte, as many aspects, become an important role on atherosclerosis disease treatment.
Study Design
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Study Type : Interventional  (Clinical Trial)
Estimated Enrollment : 60 participants
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Single (Outcomes Assessor)
Primary Purpose: Treatment
Official Title: Effects of High Intensity Interval Training and Moderate Intensity Continuous Training on Nitric Oxide-mediated Erythrocyte Rheology
Actual Study Start Date : March 5, 2021
Estimated Primary Completion Date : September 4, 2021
Estimated Study Completion Date : September 4, 2021
Arms and Interventions
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Arm Intervention/treatment
Experimental: High intensity-interval training (HIIT)
Subjects performed HIIT (3-min intervals at 40% and 80%VO2peak) on a bicycle ergometer for 30 min/day, 5 days/week for 6 weeks.
 Behavioral: High intensity-interval training (HIIT)

Subjects performed HIIT (3-min intervals at 40% and 80%VO2peak) on a bicycle ergometer for 30 min/day, 5 days/week for 6 weeks.

Without any exercise training
Experimental: Moderate intensity-continuous (MICT)
Subjects performed MICT (sustained 60%VO 2max) on a bicycle ergometer for 30 min/day, 5 days/week for 6 weeks.
 Behavioral: Moderate intensity-continuous (MICT)
Subjects performed MICT (sustained 60%VO 2max) on a bicycle ergometer for 30 min/day, 5 days/week for 6 weeks.
No Intervention: Control group
Without any exercise training


Outcome Measures
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Primary Outcome Measures :
  1. Intracellular NO production response to exercise training. [ Time Frame: 8 weeks ]
    Added different inhibitors or agonists to investigate the effects of exercise training on NO production mediated by eNOS-NO pathway.

  2. Intracellular ROS production response to exercise training. [ Time Frame: 8 weeks ]
    Added different inhibitors or agonists to investigate the effects of exercise training on ROS production mediated by eNOS-NO pathway.

  3. The levels of eNOS, p-eNOS and Band-3 response to exercise training. [ Time Frame: 8 weeks ]
    Detect the following protein levels: eNOS, p-eNOS and Band-3, by the western blots.


Secondary Outcome Measures :
  1. Determination of erythrocyte biological markers by Flow Cytometry [ Time Frame: 8 weeks ]
    Using the Flow Cytometry to detect the following markers: CD242, CD239, NADPH oxidase 2 (Nox2) and Arginase 1 (Arg1).

  2. Erythrocyte deformability [ Time Frame: 8 weeks ]
    Isolated erythrocyte first, then assess erythrocyte deformability (elongation index ) by using laser assisted optical rotational red cell analyzer (LoRRca).

  3. Cardiopulmonary fitness [ Time Frame: 8 weeks ]
    To assess cardiopulmonary fitness, cardiopulmonary exercise test (CPET) on a cycle ergometer was performed 4 days before and after the intervention. All subjects underwent exercise with a mask to measured oxygen consumption (VO2) breath by breath using a computer-based system.


Eligibility Criteria
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Ages Eligible for Study:   20 Years to 30 Years   (Adult)
Sexes Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes
Criteria

Inclusion Criteria:

  • Having a sedentary lifestyle (without regular exercise, exercise frequency ≤ once weekly, duration < 20 min).

Exclusion Criteria:

  • Exposed to high altitudes (> 3000 m) for at least 1 year.
  • Smoker
  • Taking medications or vitamins
  • Having any cardiopulmonary/hematological risk.
Contacts and Locations
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Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT04823429


Contacts
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Contact: Jong-Shyan Wang, PhD +886-3-2118800 ext 5748 s5492@mail.cgu.edu.tw

Locations
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Taiwan
Chang Gung University Recruiting
Taoyuan, Taiwan, 333
Contact: Jong-Shyan Wong, PhD         
Sponsors and Collaborators
Chang Gung Memorial Hospital
Investigators
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Principal Investigator: Jong-Shyan Wang, PhD Chang Gung Memorial Hospital
More Information
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Publications:

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Responsible Party: Jong-Shyan Wang, Principal Investigator, Chang Gung Memorial Hospital
ClinicalTrials.gov Identifier: NCT04823429    
Other Study ID Numbers: 202000448A3
First Posted: March 30, 2021    Key Record Dates
Last Update Posted: March 30, 2021
Last Verified: March 2021
Individual Participant Data (IPD) Sharing Statement:
Plan to Share IPD: No

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Studies a U.S. FDA-regulated Drug Product: No
Studies a U.S. FDA-regulated Device Product: No
Keywords provided by Jong-Shyan Wang, Chang Gung Memorial Hospital:
Erythrocyte
eNOS
High intensity interval training
deformability