A Study to Evaluate the Clinical Performance, Safety and Pharmacology Effect of EscharEx in Patients With Lower Leg Ulcers

• ClinicalTrials.gov Identifier: NCT04817228
• Recruitment Status: Recruiting
• First Posted: March 26, 2021
• Last Update Posted: September 2, 2021
• Sponsor: MediWound Ltd
• Information provided by (Responsible Party): MediWound Ltd

Study Description

Brief Summary:

This study will be a multicenter, prospective, open label, one-arm study intended to assess the clinical performance and safety of EX-02 in debridement of lower leg ulcers: Venous Leg Ulcers (VLU) and Diabetic Foot Ulcer (DFU). In addition, the pharmacology effect of EX-02 will be assessed.

Lower leg ulcer size between 2 cm2 and 80 cm2 (surface area).

Condition or disease	Intervention/treatment	Phase
Venous Leg Ulcer; Diabetic Foot Ulcer	Drug: EscharEx (EX-02 formulation)	Phase 2

Detailed Description:

Up to fifteen (15) adult patients with >50% non-viable tissue (necrotic/ slough/ fibrin) on the ulcer will be enrolled from up to 3 sites.

Each patient will go through the following steps during the trial:

1. Screening and Inform Consent signing - screening visit duration will be up to 1 week and will include: recording demographics, medical history and concomitant medications, vital signs, physical examination, clinical laboratory tests, wound photography and assessments and pain assessment questionnaires. Wound fluid sampling and 3 mm punch biopsy (from the wound edge area) will be taken during screening.

2. Daily visits period (up to 8 daily site visits within up to 14 days), including an additional visit, 48 hrs after last daily visit for wound fluid sampling. During the Daily visits period, the patient will arrive daily to site visits. During each visit, adverse events (AEs), concomitant medications, vital signs, and pain will be assessed and recorded, the wound will be washed, photographed and assessed for wound size and for % of nonviable tissue by clinical assessment. During the daily visits period, the peri-wound will be well protected with topical barrier (e.g zinc oxide paste). For VLU, compression therapy must be used throughout daily visits period.

   The investigator will clinically assess complete debridement, upon achieving a viable wound bed after removal of all non-viable tissue, suitable for initiation of the wound healing stage.

   Patients will be treated with EX-02 for up to 8 applications (up to 8 visits) or until complete debridement is achieved, whichever occurs first. Duration of each application will be 24±3 hours. On weekends occurring during the daily visits period the wound will be dressed with a non-active dressing (e.g. foam).

   Hydrogels as well as methods of active debridement (either surgical, mechanical, biological, enzymatic or other dressings), grafting and V.A.C are not allowed during this period and while daily visits are ongoing.

   Fluid samples will be taken from the EX-02 treated wound before the first treatment and once the wound has reached complete debridement or daily visits completed, whichever occurred first. Punch biopsies from the wound edge will be taken before and at the end of the debridement to assess for biofilm.

   Clinical lab tests and physical examination as well as pain assessments and vital signs will be performed at the end of daily visits period (once the wound has reached complete debridement or daily visits completed, whichever occurred first).

   For patients who achieved complete debridement, wound closure could be considered using grafting/autograft procedure.

   After completion of the daily visits period (either if complete debridement was achieved, or after 8 daily applications were performed) patients will start the weekly follow- up period.

3. Weekly follow- up period (2 visits in 2 weeks) - the 2 weekly follow up period should be performed at the clinical site. During each weekly visit, safety parameters will be recorded (AEs, concomitant medications, pain, vital signs), the wound will be washed, photographed and assessed for wound size and for % of nonviable tissue.

For wounds which did not reach complete debridement during the daily visits period, the investigator should continue to persue complete debridement using surgical or non- surgical debridement tools (SOC), per the investigators discretion.

Compression therapy must be used throughout this period for VLU patients and as determined by investigator to be appropriate for DFU.

The overall duration of the study for each patient will be approximately 4-5 weeks.

Study Design

• Study Type: Interventional (Clinical Trial)
• Estimated Enrollment: 15 participants
• Allocation: N/A
• Intervention Model: Single Group Assignment
• Masking: None (Open Label)
• Primary Purpose: Treatment
• Official Title: A Prospective Study Performed to Evaluate the Clinical Performance (Debridement), Safety and Pharmacology Effect of EscharEx (EX-02 Formulation) in Patients With Lower Leg Ulcers (VLU and DFU)
• Actual Study Start Date: April 21, 2021
• Estimated Primary Completion Date: December 2021
• Estimated Study Completion Date: February 2022

Arms and Interventions

Arm	Intervention/treatment
Experimental: EX-02 Gel; The powder of EX-02 (8.1 g in each unit) should be reconstituted with 20 g sterile water for injection (WFI) to obtain 5% EX-02. EX-02 5% gel will be topically applied on a wound surface of up to 80 cm2 for 24±3 hours, up to 8 consecutive applications	Drug: EscharEx (EX-02 formulation); EscharEx (EX-02 formulation) gel is topically applied on a wound surface of up to 80 cm2 , up to 8 consecutive applications or until a complete debridement is achieved, whichever occurs first.

Outcome Measures

Primary Outcome Measures :

1. Incidence of complete debridement [ Time Frame: up to 14 days ]
   Incidence of complete debridement assessed clinically
2. Time to complete debridement [ Time Frame: up to 14 days ]
   Time to complete debridement assessed clinically
3. Change from baseline in wound fluid biomarkers [ Time Frame: up to 14 days ]
   Wound healing biomarkers (MMP2, MMP9, HNE, PDGF, IL8, TNFR1) distribution in wound fluid will be compared before and after completion of treatment
4. Change from baseline in wound biofilm presence before and after treatment [ Time Frame: up to 14 days ]
   Biofilm presence in wound will be compared before and after completion of treatment, using punch biopsy from wound

Eligibility Criteria

• Ages Eligible for Study: 18 Years to 90 Years (Adult, Older Adult)
• Sexes Eligible for Study: All
• Accepts Healthy Volunteers: No

Criteria

Inclusion Criteria:

1. Patients, men or women, between 18 and 90 years of age,
2. Patients with a VLU (determined by medical history, physical examination or an ultrasound scan demonstrating venous insufficiency) or DFU (determined by medical history, and physical examination),
3. Wound is present for at least 4 weeks but no longer than 2 years,
4. The necrotic/slough/fibrin non-viable tissue area is at least 50% of wound area (assessed by clinical evaluation),
5. Target wound surface area is in the range of 2-80 cm2,
6. Patient understands the nature of the procedure, is able to adhere to the protocol regimen, and provides a written informed consent prior to any study procedure.

Exclusion Criteria:

1. Patients with more than one leg ulcer, on the leg of the target wound, with an area greater than or equal to 2cm2,
2. Signs of clinically significant infection including purulent discharge, deep-tissue abscess, erysipelas, cellulitis at the surrounding skin, etc.,
3. Severely damaged skin at the per wound (e.g. abrasion, exfoliation) extending >2 cm around the wound's edge,
4. Presence of gangrene, signs of systemic infection, sepsis, or osteomyelitis during screening phase,
5. Clinical suspicion of skin cancer (e.g. BCC, SCC, melanoma, sarcoma), which was not ruled out by biopsy (in this case a biopsy will be performed before screening),
6. Patients suffering from chronic skin disorders (Idiopathic Pruritus, Psoriasis, Panniculitis, Pyoderma Gangrenosum, etc.) that might deteriorate as a result of local trauma or debridement,
7. Wound has sinus tracts or tunnels extending under the skin >2 cm surrounding subcutaneous structures, or penetrating into joint capsule,
8. A significant decrease in the arterial blood flow of the extremity,
9. Patients with pre-enrolment wounds which are covered by eschar heavily saturated with iodine or by silver, silver sulfadiazine (SSD) pseudoeschar (i.e. pseudoeschar as a result of SSD treatment),
10. History of allergy or atopic disease or a known sensitivity to pineapples, papaya, bromelain or papain, as well as known sensitivity to latex proteins (known as latex-fruit syndrome), bee venom or olive tree pollen,
11. Patients with poor nutritional status: albumin < 3.0 g/dl, poorly controlled Diabetes Mellitus (for diabetic patients; HbA1c > 12%), anemia (hemoglobin<8 g/dL), a leukocyte counts < 4,000/ μl or >15000/μl, Neutrophils < 1500/ul, platelets <100,000/μl, abnormal liver function (AST, ALT>2 x upper limit of normal range), total bilirubin > 1.5 mg/dl, renal failure (Cr > 2.5 mg/dl), BMI>45,
12. Patients undergoing renal or peritoneal dialysis,
13. Any condition that would preclude safe participation in the study, e.g. evidence of significant or unstable cardiovascular, pulmonary, liver, hematological, immunological, positive for COVID-19 or neoplastic disease, or any immediate life threatening condition,
14. Recent history (less than 6 months) of myocardial infarction (MI) or concurrent acute injury or disease that might compromise the patient's welfare,
15. Patient is currently receiving, or has received at any time within the past 30 days any medications or treatments known to affect the wound healing processes; these include, chronic systemic steroid intake ( >10 mg/ day) with topical skin changes (i.e. thin, fragile skin with multiple heamatomas or previous laceration history) immuno-suppressive drugs, radiation therapy, immunomodulating medications and chemotherapy,
16. Mentally incapacitated adults who are incapable of giving legal consent (e.g. dementia, psychiatric patients, etc.),
17. Concurrent use of non-approved drugs or alcohol abuse,
18. Pregnant women (positive pregnancy test) or nursing mothers,
19. Exposure to investigational intervention within 3 months prior to enrollment, or anticipated participation in another investigational drug trial or other intervention trial, while enrolled into the study

Contacts and Locations

Contacts

Contact: Aya Ben-Yaakov, Dr.; +972-77-971-4173; ayaby@mediwound.com

Contact: Keren David-Zarbiv; +972-77-9714103; kerend@mediwound.com

Locations

United States, Florida

Barry University foot and ankle institute; Recruiting

Tamarac, Florida, United States, 33133

Contact: Maria Swartz 954-258-9409 MSwartz@barry.edu

Principal Investigator: Howard Petusevsky

United States, North Carolina

University of North Carolina; Not yet recruiting

Chapel Hill, North Carolina, United States, 27599-7212

Contact: Zachary Gortler 919-843-1268 zachary_gortler@med.unc.edu

Principal Investigator: Stephen Heisler

United States, South Carolina

Prisma Health-Upstate; Not yet recruiting

Greenville, South Carolina, United States, 29601

Contact: Robin Bradley 864-454-2818 Robin.Bradley@prismahealth.org

Principal Investigator: Robert Klein, MD

Sponsors and Collaborators

MediWound Ltd

Investigators

• Study Chair: Lior Rosenberg, Prof.; MediWound Ltd

More Information

• Responsible Party: MediWound Ltd
• ClinicalTrials.gov Identifier: NCT04817228
• Other Study ID Numbers: MW 2020-08-01
• First Posted: March 26, 2021
• Last Update Posted: September 2, 2021
• Last Verified: March 2021

• Studies a U.S. FDA-regulated Drug Product: Yes
• Studies a U.S. FDA-regulated Device Product: No

Additional relevant MeSH terms:

Diabetic Foot; Varicose Ulcer; Foot Ulcer; Leg Ulcer; Ulcer; Pathologic Processes; Diabetic Angiopathies; Vascular Diseases; Cardiovascular Diseases; Skin Ulcer; Skin Diseases; Diabetes Complications; Diabetes Mellitus; Endocrine System Diseases; Diabetic Neuropathies; Foot Diseases; Varicose Veins