Detection of the Most Common Microorganism of Infective Endocarditis in Assiut University Hospital

• ClinicalTrials.gov Identifier: NCT03487653
• Recruitment Status: Unknown
• First Posted: April 4, 2018
• Last Update Posted: July 6, 2018
• Sponsor: Safaa Samir
• Information provided by (Responsible Party): Safaa Samir, Assiut University

Study Description

Brief Summary:

Indocarditis is an endogenous infection acquired when organisms entering the blood

stream establish on the heart valves, therefore, any bacteremia can potentially result in endocarditis.

Infective endocarditis is an uncommon disease that often presents as pyrexia of unknown origin.

The mortality rate in endocarditis was very high before the antibiotic era, even now a day, the

mortality rate is around 20%(1).A variety of microorganisms can cause IE; staphylococci and streptococci account for the majority of cases. Staphylococcal IE is a common cause of healthcare-associated IE ; streptococcal IE is a common cause of community-acquired IE. Common bacterial pathogens include Staphylococcus aureus , Viridans group streptococci , Enterococcus, Coagulase-negative staphylococci , Streptococcus bovis , other streptococci , gram-negative bacteria, HACEK organisms in this category include a number of fastidious gram-negative bacilli: Haemophilus aphrophilus(subsequently called Aggregatibacter aphrophilus and Aggregatibacter paraphrophilus); Actinobacillus actinomycetemcomitans (subsequently called Aggregatibacter actinomycetemcomitans); Cardiobacterium hominis; Eikenella corrodens; and Kingella kingae , and fungi (1,2). A variable proportion of IE remain blood culture- negative (1-4). Most clinically significant bacteremias are detected within 48 hours; common and fastidious pathogens (such as members of the HACEK group) may be detected within five days of incubation with modern automated blood culture detection systems. The optimal volume of blood for each blood culture in adults is 20 ml.

Zoonotic agents, such as Coxiella burnetii, Brucella spp., and Bartonella spp. were frequently detected in North Africa and identified as causes of infective endocarditis (IE) in Egypt (3,4).Blood culture is the most important investigation for diagnosing infective endocarditis

andto know the prevalence rate of different bacteria and their antibiotic sensitivity pattern.Positive blood culture is the cornerstone of microbiological diagnosis of IE; three sets of blood cultures detect 96 to 98 percent of bacteremia. At least three sets of blood cultures should be obtained from separate venipuncture sites prior to initiation of antibiotic therapy. Patients with IE typically have continuous bacteremia; therefore, blood cultures may be collected at any time and need not necessarily be obtained at the time of fever or chills.

MATERIAL and METHOD A total of 150 blood cultures were received from 50 clinically diagnosed cases of bacterial endocarditis .

Blood sample was collected under all aseptic precautions.

Condition or disease	Intervention/treatment
Infective Endocarditis	Biological: microbiology:Blood culture and sensitvity test .Serology

Study Design

• Study Type: Observational
• Estimated Enrollment: 50 participants
• Observational Model: Other
• Time Perspective: Prospective
• Official Title: Detection of the Most Common Micro Organism of Infective Endocarditis in Assiut University Hospital
• Estimated Study Start Date: December 1, 2018
• Estimated Primary Completion Date: March 1, 2019
• Estimated Study Completion Date: December 30, 2019

Groups and Cohorts

Intervention Details:

• Biological: microbiology:Blood culture and sensitvity test .Serology
  Gram stain ,Blood culture ,sensitivity test ,biochemical reactions and serological study will be done

Outcome Measures

Primary Outcome Measures :

1. Detection of the most common micro organism of infective endocarditis [ Time Frame: 2years ]
   Detection of the most common microorganism of infective endocarditis

Eligibility Criteria

• Ages Eligible for Study: Child, Adult, Older Adult
• Sexes Eligible for Study: All
• Accepts Healthy Volunteers: No
• Sampling Method: Probability Sample

Study Population

Detection of the most common organism of infective endocarditis

Criteria

Inclusion Criteria:patients that are clinically diagnosed as infective endocarditis before starting antibiotics

Exclusion Criteria:patients that receive antibiotics before taking the blood sample

Contacts and Locations

No Contacts or Locations Provided

More Information

• Responsible Party: Safaa Samir, Principle investigator, Assiut University
• ClinicalTrials.gov Identifier: NCT03487653
• Other Study ID Numbers: Assiut university hosbital
• First Posted: April 4, 2018
• Last Update Posted: July 6, 2018
• Last Verified: March 2018

Individual Participant Data (IPD) Sharing Statement:

• Plan to Share IPD: Undecided

• Studies a U.S. FDA-regulated Drug Product: No
• Studies a U.S. FDA-regulated Device Product: No

Additional relevant MeSH terms:

Endocarditis, Bacterial; Endocarditis; Heart Diseases; Cardiovascular Diseases; Bacterial Infections; Bacterial Infections and Mycoses; Infections; Cardiovascular Infections