Substrate Metabolism During Exercise Following Ingestion of Ethanol

• ClinicalTrials.gov Identifier: NCT03404947
• Recruitment Status: Completed
• First Posted: January 19, 2018
• Last Update Posted: March 6, 2019
• Sponsor: University of Bath
• Information provided by (Responsible Party): James Betts, University of Bath

Study Description

Brief Summary:

This study will investigate how ethanol (pure alcohol) influences carbohydrate and fat metabolism during prolonged, moderate intensity exercise. Participants will perform two bouts of cycling exercise with or without prior ingestion of ethanol, in a randomised order, separated by one week.

Condition or disease	Intervention/treatment	Phase
Exercise Metabolism Following Ethanol Ingestion	Dietary Supplement: Ethanol	Not Applicable

Detailed Description:

Very little research has investigated the influence of ethanol on metabolism during prolonged exercise. Evidence suggests that delivery of fat to the working muscles during exercise may be limited with ethanol and therefore this may shift the relative proportions of energy derived from muscle stores of carbohydrate as a result (Jorfeldt & Juhlin-Dannfelt, 1976).

The current study aims to investigate how a small-moderate dose of ethanol influences where energy is derived from during a prolonged bout of moderate intensity cycling exercise.

Participants will visit the lab on three occasions, once for preliminary measurements of fitness and body composition, and twice for the experimental bouts of exercise. Body composition will be assessed using a dual-energy x-ray absorptiometry (DEXA) scan, which will measure lean mass, fat mass, and bone mineral density. Participants will then perform an incremental maximal oxygen uptake test to determine intensity for the experimental bouts of exercise.

The two experimental bouts of exercise include cycling on an exercise bike for 2 hours at 55% of their maximal oxygen uptake (i.e. fitness) following 1 hours rest. This will be performed under two conditions: with and without ethanol ingestion. Pre and post exercise muscle biopsies will be collected to assess muscle metabolism, with regular blood samples and expired breath samples being collected to further investigate fuel delivery and use by the working tissues. The experimental bouts of exercise will be performed 1 week apart in a randomised order.

Study Design

• Study Type: Interventional (Clinical Trial)
• Actual Enrollment: 8 participants
• Allocation: Randomized
• Intervention Model: Crossover Assignment
• Masking: None (Open Label)
• Primary Purpose: Basic Science
• Official Title: Substrate Metabolism During 2 Hours of Moderate Intensity Exercise Following Ingestion of Ethanol in Man
• Actual Study Start Date: November 13, 2017
• Actual Primary Completion Date: June 29, 2018
• Actual Study Completion Date: June 29, 2018

Arms and Interventions

Arm	Intervention/treatment
Experimental: Ethanol; Participants will ingest ethanol (in the form of 40% ethanol) at an ingestion rate of 0.1 grams/kg lean body mass/hour in a solution with water.	Dietary Supplement: Ethanol; For one exercise session, participants will be asked to ingest a quantity of ethanol (in the form of vodka) sufficient to maximally stimulate liver alcohol metabolism.
No Intervention: No Ethanol; Participants will ingest a volume matched beverage of water only.

Outcome Measures

Primary Outcome Measures :

1. Change in muscle glycogen content [ Time Frame: Change in muscle glycogen content over 2 hours of moderate intensity cycling exercise ]
   Muscle biopsy and subsequent biochemical analysis
2. Blood glucose concentration [ Time Frame: Blood samples will be taken every 15 minutes during rest and the first hour of exercise, they will be taken every 30 minutes in the second hour of exercise. ]
   Venous blood samples will be taken and biochemically analysed for blood glucose concentration.
3. Blood non-esterified fatty acid (NEFA) concentration [ Time Frame: Blood samples will be taken every 15 minutes during rest and the first hour of exercise, they will be taken every 30 minutes in the second hour of exercise. ]
   Venous blood samples will be taken and biochemically analysed for NEFA concentration.

Secondary Outcome Measures :

1. Blood ethanol concentration [ Time Frame: Blood samples will be taken every 15 minutes during rest and the first hour of exercise, they will be taken every 30 minutes in the second hour of exercise. ]
   Venous blood samples will be taken and biochemically analysed for blood ethanol concentration.
2. Blood lactate concentration [ Time Frame: Blood samples will be taken every 15 minutes during rest and the first hour of exercise, they will be taken every 30 minutes in the second hour of exercise. ]
   Venous blood samples will be taken and biochemically analysed for blood lactate concentration.
3. Carbohydrate Oxidation [ Time Frame: Expired breath samples will be collected at baseline before beverage consumption and again after 1 hours rest. Samples will collected every 15 minutes during the first hour of exercise and every 30 minutes during the second hour ]
   Carbohydrate oxidation will be determined through indirect calorimetry via the douglas bag technique.
4. Fat Oxidation [ Time Frame: Expired breath samples will be collected at baseline before beverage consumption and again after 1 hours rest. Samples will collected every 15 minutes during the first hour of exercise and every 30 minutes during the second hour ]
   Fat oxidation will be determined through indirect calorimetry via the douglas bag technique.

Eligibility Criteria

• Ages Eligible for Study: 18 Years to 30 Years (Adult)
• Sexes Eligible for Study: Male
• Accepts Healthy Volunteers: Yes

Criteria

Inclusion Criteria:

• Habitual alcohol consumer (average 1-14 units per week)
• BMI 18.5 - 29.9 kg/m2
• Be able to attend the laboratory and willing to participate in necessary protocols
• Be willing to undertake the durations of the exercise protocol (2 hours) ]
• Have the capacity and willingness to provide informed consent (oral and written)

Exclusion Criteria:

• Non-or excessive alcohol drinkers (0 or >14 units per week)
• History of substance abuse
• Habitual/previously habitual smokers
• BMI >30.0 kg/m2
• Experience contraindications to the administration of the proposed anaesthetic (Lidocaine Hydrochloride)

Contacts and Locations

Locations

United Kingdom

University of Bath

Bath, Somerset, United Kingdom, BA2 7AY

Sponsors and Collaborators

University of Bath

Investigators

• Principal Investigator: Harry Smith; University of Bath
• Principal Investigator: James Betts, PhD; University of Bath

More Information

Publications:

Jorfeldt L, Juhlin-Dannfelt A. The influence of ethanol on human splanchnic and skeletal muscle metabolism during exercise. Scand J Clin Lab Invest. 1977 Nov;37(7):609-18.

Juhlin-Dannfelt A, Ahlborg G, Hagenfeldt L, Jorfeldt L, Felig P. Influence of ethanol on splanchnic and skeletal muscle substrate turnover during prolonged exercise in man. Am J Physiol. 1977 Sep;233(3):E195-202.

Juhlin-Dannfelt A, Jorfeldt L, Hagenfeldt L, Hulten B. Influence of ethanol on non-esterified fatty acid and carbohydrate metabolism during exercise in man. Clin Sci Mol Med. 1977 Sep;53(3):205-14.

• Responsible Party: James Betts, Principal Investigator, University of Bath
• ClinicalTrials.gov Identifier: NCT03404947
• Other Study ID Numbers: ETOH-hs565
• First Posted: January 19, 2018
• Last Update Posted: March 6, 2019
• Last Verified: March 2019

Individual Participant Data (IPD) Sharing Statement:

• Plan to Share IPD: Undecided

• Studies a U.S. FDA-regulated Drug Product: No
• Studies a U.S. FDA-regulated Device Product: No

Keywords provided by James Betts, University of Bath:

Exercise; Metabolism; Nutrition

Additional relevant MeSH terms:

Ethanol; Anti-Infective Agents, Local; Anti-Infective Agents; Central Nervous System Depressants; Physiological Effects of Drugs