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Phenotypic Characterization of Alveolar Macrophages 2016 (Caract-Aires-2016) (Caract-Aires)

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ClinicalTrials.gov Identifier: NCT03250897
Recruitment Status : Unknown
Verified August 2017 by Rennes University Hospital.
Recruitment status was:  Recruiting
First Posted : August 16, 2017
Last Update Posted : August 16, 2017
Sponsor:
Information provided by (Responsible Party):
Rennes University Hospital

Study Description
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Brief Summary:
The aim of the study is to investigate by flow cytometry the phenotype of alveolar macrophages (AMs) isolated from patients with non-infectious lung diseases.

Condition or disease
Lung Diseases

Detailed Description:

Although alveolar macrophages (AMs) represent the main immune cell population in the human lungs, consensus on their phenotypic characterization is still missing.

Current translational protocols use human AMs obtained by invasive methods (broncho-alveolar lavage and lung biopsy) or differentiate macrophages from blood monocytes cultured in the presence of growth factors such as macrophage colony-stimulating factor (M-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF). The group has already published the phenotypic and functional comparison of human blood monocyte-derived macrophages differentiated with M-CSF or GM-CSF. Aim of this study is to use a multi-staining flow cytometry (FACS analysis) to better characterize the phenotype of AMs isolated from broncho-alveolar lavage fluids of patients affected by non-infectious lung diseases. The FACS profiles of AMs will be further compared to the phenotypic profile of blood monocyte-derived macrophages differentiated in the presence of GM-CSF or M-CSF. The results of this study will allow to define the most valid in vitro model of AMs in a non-infectious setting.

Study Design
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Study Type : Observational
Estimated Enrollment : 18 participants
Observational Model: Cohort
Time Perspective: Prospective
Official Title: Phenotypic Characterization of Alveolar Macrophages 2016
Actual Study Start Date : November 2016
Estimated Primary Completion Date : December 2017
Estimated Study Completion Date : September 2018

Resource links provided by the National Library of Medicine

MedlinePlus related topics: Lung Diseases

Groups and Cohorts
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Outcome Measures
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Primary Outcome Measures :
  1. To investigate by flow cytometry the phenotype of alveolar macrophages (AMs) isolated from broncho-alveolar lavage fluids of patients with non-infectious lung diseases. [ Time Frame: 1 year ]
    Characterization of membrane proteins by multi-staining flow cytometry (FACS analysis)


Secondary Outcome Measures :
  1. To compare the phenotypic profile of AMs from broncho-alveolar lavage fluids of patients with non-infectious lung diseases to that of blood monocyte-derived macrophages differentiated in the presence of GM-CSF or M-CSF and obtained from healthy donors [ Time Frame: 1 year ]

Biospecimen Retention:   Samples With DNA
Broncho-alveolar lavage fluids of patients with non-infectious lung diseases

Eligibility Criteria
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Ages Eligible for Study:   18 Years and older   (Adult, Older Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   No
Sampling Method:   Non-Probability Sample
Study Population
Patients affected by non-infectious lung diseases older than 18 years
Criteria

Inclusion Criteria:

  • Patients affected by non-infectious lung diseases and for whom a broncho-alveolar lavage is scheduled for their medical care in the pulmonary division
  • Patient Information leaflet provided
  • Written informed consent of the patient

Exclusion Criteria:

  • A suspected respiratory infection
Contacts and Locations
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Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT03250897


Contacts
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Contact: Stéphane Jouneau, Professor 0299282481 stephane.jouneau@chu-rennes.fr

Locations
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France
Pneumology Division Recruiting
Rennes, France, 35033
Contact: Stéphane Jouneau, Professor    +33 2 99 28 24 81    stephane.jouneau@chu-rennes.fr   
Sponsors and Collaborators
Rennes University Hospital
Investigators
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Principal Investigator: Stéphane Jouneau, Professor Rennes University Hospital
More Information
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Responsible Party: Rennes University Hospital
ClinicalTrials.gov Identifier: NCT03250897    
Other Study ID Numbers: 35RC16_3055_Caract-Aires-2016
First Posted: August 16, 2017    Key Record Dates
Last Update Posted: August 16, 2017
Last Verified: August 2017

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Studies a U.S. FDA-regulated Drug Product: No
Studies a U.S. FDA-regulated Device Product: No
Keywords provided by Rennes University Hospital:
Alveolar macrophages
Blood monocyte-derived macrophages
FACS analysis
Broncho-alveolar lavage fluids
Non-infectious lung diseases
Additional relevant MeSH terms:
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Lung Diseases
Respiratory Tract Diseases