Effect of an Alpha-linolenic Acid-rich Supplement on Ketogenesis and Plasma Fatty Acids
|ClinicalTrials.gov Identifier: NCT02410161|
Recruitment Status : Completed
First Posted : April 7, 2015
Results First Posted : February 18, 2019
Last Update Posted : February 18, 2019
Background: As the main alternative fuel to glucose for the brain, increased plasma ketones could potentially help compensate for brain glucose hypometabolism occurring during aging. The precursor long-chain n-3 polyunsaturated fatty acid (PUFA), α-linolenic acid (ALA), is normally mostly β-oxidized and so could potentially be used to stimulate ketogenesis in humans.
Objective: To compare the impact of an ALA-rich supplement on the ketogenic response in young and older healthy adults.
Design: Ten young and ten older adults will consume a flaxseed oil supplement providing 2 g/d of ALA for 4 weeks. Plasma ketones, free fatty acids, triglycerides, glucose and insulin will be measured over 6 h during two metabolic study days, one before and one at the end of the supplementation.
Hypothesis: ALA-rich supplement for 4 weeks will increase ketone production in both groups.
|Condition or disease||Intervention/treatment||Phase|
|Healthy||Dietary Supplement: alpha-linolenic acid-rich supplement||Not Applicable|
|Study Type :||Interventional (Clinical Trial)|
|Actual Enrollment :||20 participants|
|Intervention Model:||Single Group Assignment|
|Intervention Model Description:||Dietary Supplement: alpha-linolenic acid-rich supplement|
|Masking:||None (Open Label)|
|Primary Purpose:||Basic Science|
|Official Title:||Effect of an Alpha-linolenic Acid-rich Supplement on Ketogenesis and Plasma n-3 Polyunsaturated Fatty Acids in Young Compared to Older Adults|
|Study Start Date :||July 2013|
|Actual Primary Completion Date :||May 2014|
|Actual Study Completion Date :||May 2014|
Experimental: 4 week ALA treatment
Participants will receive the alpha-linolenic acid-rich supplement (1000mg 4 times par day) for 4 weeks
Dietary Supplement: alpha-linolenic acid-rich supplement
each participant consumed one 1000 mg capsule of flaxseed oil four times per day for 4 weeks, providing a total of 2 g/d of ALA. The capsules used were a commercially available flaxseed oil supplement containing ALA at 56% of total fatty acids (Jamieson, Toronto, ON, Canada). The other main fatty acids present in flaxseed oil include linoleic acid (18%), oleic acid (16%), palmitic and stearic acid (10% combined).
- Ketone Production [ Time Frame: After 4 weeks ]Total Ketone (acetoacetate + beta-hydroxybutyrate) concentration in plasma in average during the metabolic study day, measured hourly between 1 and 6h after breakfast
- Plasma Glucose [ Time Frame: After 4 weeks ]Glucose measured in plasma in average during the metabolic study day, measure hourly between 0 and 6 h after breakfast
- Plasma Triglycerides [ Time Frame: After 4 weeks ]Triglycerides measured in plasma in average during the metabolic study day, measured hourly between 1 to 6h after breakfast
- Plasma Free Fatty Acids [ Time Frame: 4 weeks ]Free fatty acids measured in plasma in average during the metabolic study day, measured hourly between 1 to 6h after breakfast
- Insulin Concentration in Plasma [ Time Frame: after 4 weeks ]Insulin measured in plasma in average during the metabolic study day, measured hourly between 0 ans 6 h after breakfast
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT02410161
|Principal Investigator:||Stephen Cunnane, PhD||Université de Sherbrooke|