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Influence of Sex and Training on de Novo Muscle Protein Synthesis (TUT)

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ClinicalTrials.gov Identifier: NCT04887883
Recruitment Status : Completed
First Posted : May 14, 2021
Last Update Posted : May 14, 2021
Sponsor:
Collaborator:
Natural Sciences and Engineering Research Council, Canada
Information provided by (Responsible Party):
Daniel Moore, University of Toronto

Tracking Information
First Submitted Date May 5, 2021
First Posted Date May 14, 2021
Last Update Posted Date May 14, 2021
Actual Study Start Date January 1, 2019
Actual Primary Completion Date September 1, 2019   (Final data collection date for primary outcome measure)
Current Primary Outcome Measures
 (submitted: May 10, 2021)
Dietary fate of amino acids into myofibrillar and sarcoplasmic proteins [ Time Frame: The change in muscle MPE at 0 and 24 h after acute resistance exercise before and after training ]
MPE
Original Primary Outcome Measures Same as current
Change History No Changes Posted
Current Secondary Outcome Measures
 (submitted: May 10, 2021)
  • Amino acid transporter protein content [ Time Frame: 0 and 24 hours after acute resistance exercise before and after training ]
    Arbitrary units
  • mTOR localization with capillaries [ Time Frame: 0 and 24 hours after acute resistance exercise before and after training ]
    Pearson's r
Original Secondary Outcome Measures Same as current
Current Other Pre-specified Outcome Measures Not Provided
Original Other Pre-specified Outcome Measures Not Provided
 
Descriptive Information
Brief Title Influence of Sex and Training on de Novo Muscle Protein Synthesis
Official Title Influence of Sex, Acute Resistance Exercise and Training on de Novo Muscle Protein Synthesis
Brief Summary Acute exercise increases the incorporation of dietary amino acids into de novo myofibrillar proteins after a single meal in controlled laboratory studies in males. It is unclear if this extends to free-living settings or is influenced by training or sex. Over 24 h in a free-living setting, the investigators determined the effect of training status and sex on dietary phenylalanine incorporation into contractile myofibrillar and noncontractile sarcoplasmic proteins after exercise.
Detailed Description

Exogenous (e.g. diet-derived) amino acids increase muscle protein synthesis and provide the building blocks for growth. While traditional infusion studies can measure the synthesis of total mixed muscle or fraction-specific protein synthetic rates, the metabolic fate of dietary amino acids can only be assessed by measuring the incorporation of a labelled amino acid (i.e. L-[1-13C]phenylalanine) into muscle protein through the oral ingestion of a intrinsically labelled food source (e.g. milk protein) . This technique has revealed in controlled laboratory settings that dietary amino acids, and not endogenous amino acids recycled from intracellular protein breakdown, may be preferentially utilized as precursors for muscle and whole body protein synthesis Therefore, it is important to characterize the incorporation of diet-derived amino acids over a 24-h post-exercise recovery period to determine how RE influences their utilization as precursors for the synthesis of new muscle proteins. The investigators are unaware of any studies that have examined the utilization of dietary amino acids for de novo muscle protein synthesis in females, highlighting an urgent need to rectify the sex-disparity in exercise-related research.

Protein requirements during resistance training have been suggested to be highest at training onset with evidence suggesting moderate daily intakes (~1.2-1.4 g·kg·d-1) can support chronic adaptations, although recent suggestions are that slightly higher intakes (~1.6 g·kg·d-1) may optimize lean mass growth. Resistance training is associated with a reduction in whole-body protein turnover but an increased net protein balance suggesting a greater efficiency of whole-body amino acid utilization with training in males , although whether this also extends to females is unknown. Acute RE and chronic training has been reported to increase intracellular amino acid recycling in the fasted state, which would be consistent with an increased amino acid efficiency. To date, however, no study has investigated whether the post-exercise incorporation of dietary amino acids into myofibrillar and sarcoplasmic proteins in a free-living setting is modified by training and/or sex.

The primary aim of the present study was to determine the dietary fate of amino acids into contractile myofibrillar and noncontractile sarcoplasmic muscle proteins after acute RE in the untrained and trained state over 24 h in a free-living setting. The investigators hypothesized that, irrespective of sex, acute RE would increase dietary amino acid incorporation in myofibrillar proteins in the untrained state with training leading to an attenuated increase suggestive of a reduced reliance on dietary amino acids in the trained state.

Study Type Observational
Study Design Observational Model: Cohort
Time Perspective: Retrospective
Target Follow-Up Duration Not Provided
Biospecimen Retention:   Samples With DNA
Description:
Muscle biopsies, blood and saliva
Sampling Method Non-Probability Sample
Study Population : Ten recreationally active and healthy young males and females
Condition
  • Amino Acids
  • Dietary Protein
  • Sex
  • Resistance Exercise
Intervention Behavioral: Resistance exercise
Participants performed 8 weeks of resistance training and muscle biopsies were taken before and 24 h before and after the program.
Study Groups/Cohorts
  • Males
    10 young healthy biological males aged 18 - 30 y
    Intervention: Behavioral: Resistance exercise
  • Females
    10 young healthy biological females aged 18 - 30 y
    Intervention: Behavioral: Resistance exercise
Publications * Not Provided

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Recruitment Information
Recruitment Status Completed
Actual Enrollment
 (submitted: May 10, 2021)
20
Original Actual Enrollment Same as current
Actual Study Completion Date September 1, 2019
Actual Primary Completion Date September 1, 2019   (Final data collection date for primary outcome measure)
Eligibility Criteria

Inclusion Criteria:

  • Participants were included in the study after reporting not engaging in whole body RE or plyometrics in the past 3 months and had a BMI between 18.5-27.5 kg/m2.
  • Females were included if they had a regular menstrual cycle with the last 3 months and

Exclusion Criteria:

  • Participants were excluded if they: i) consumed tobacco and/or illicit anabolic drug use (e.g. testosterone, growth hormones); ii) were a vegan or had a nut allergy and; iii) participated in a study within the past year involving stable isotopes.
  • Females were excluded if they used oral contraceptives and/or discontinued their use within the last 3 months.
Sex/Gender
Sexes Eligible for Study: All
Ages 18 Years to 30 Years   (Adult)
Accepts Healthy Volunteers Yes
Contacts Contact information is only displayed when the study is recruiting subjects
Listed Location Countries Canada
Removed Location Countries  
 
Administrative Information
NCT Number NCT04887883
Other Study ID Numbers TUT study
RGPIN-2015-04521 ( Other Grant/Funding Number: NSERC Discovery Grant )
Has Data Monitoring Committee No
U.S. FDA-regulated Product
Studies a U.S. FDA-regulated Drug Product: No
Studies a U.S. FDA-regulated Device Product: No
IPD Sharing Statement
Plan to Share IPD: No
Responsible Party Daniel Moore, University of Toronto
Study Sponsor University of Toronto
Collaborators Natural Sciences and Engineering Research Council, Canada
Investigators
Principal Investigator: Daniel Moore, PhD University of Toronto
PRS Account University of Toronto
Verification Date May 2021