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A Combination Efficacy Study in Africa of Two DNA-MVA-Env Protein or DNA-Env Protein HIV-1 Vaccine Regimens With PrEP (PrEPVacc)

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Know the risks and potential benefits of clinical studies and talk to your health care provider before participating. Read our disclaimer for details.
 
ClinicalTrials.gov Identifier: NCT04066881
Recruitment Status : Not yet recruiting
First Posted : August 26, 2019
Last Update Posted : August 26, 2019
Sponsor:
Collaborators:
Imperial College London
University College, London
International AIDS Vaccine Initiative
EuroVacc Foundation
Medical Research Council, South Africa
National Institute for Medical Research, Tanzania
Muhimbili University of Health and Allied Sciences
Instituto Nacional de Saúde, Mozambique
Ludwig-Maximilians - University of Munich
King's College London
Centre Hospitalier Universitaire Vaudois
Karolinska Institutet
CONRAD
Gilead Sciences
Information provided by (Responsible Party):
Prof Pontiano Kaleebu, MRC/UVRI and LSHTM Uganda Research Unit

Tracking Information
First Submitted Date  ICMJE August 8, 2019
First Posted Date  ICMJE August 26, 2019
Last Update Posted Date August 26, 2019
Estimated Study Start Date  ICMJE January 2020
Estimated Primary Completion Date March 2023   (Final data collection date for primary outcome measure)
Current Primary Outcome Measures  ICMJE
 (submitted: August 21, 2019)
  • Incident HIV infection [ Time Frame: after week 26 ]
    HIV acquisition by a participant who completed three immunisations and was HIV negative at week 26.
  • Incident HIV infection [ Time Frame: week 0-26 ]
    HIV acquisition at or before week 26 by a participant who was HIV negative at enrolment
  • A clinical decision to discontinue the vaccine regimen for an adverse event that is considered related to product [ Time Frame: week 0-48 ]
    A clinical decision to discontinue the vaccine regimen for an adverse event that is considered related to product
  • A clinical decision to discontinue PrEP regimen for an adverse event that is considered related to product [ Time Frame: week 0-26 ]
    A clinical decision to discontinue PrEP regimen for an adverse event that is considered related to product
Original Primary Outcome Measures  ICMJE Same as current
Change History No Changes Posted
Current Secondary Outcome Measures  ICMJE
 (submitted: August 21, 2019)
  • Grade 3 and worse solicited clinical and laboratory adverse events [ Time Frame: week 0-74 ]
    Grade 3 and worse solicited clinical and laboratory adverse events
  • Discontinuation or interruption of vaccine regimen [ Time Frame: week 0-74 ]
    A clinical decision to discontinue or interrupt the vaccine regimen for an adverse event that is considered related to product
  • Discontinuation or interruption of PrEP [ Time Frame: week 0-26 ]
    A clinical decision to discontinue or interrupt the PrEP regimen for an adverse event that is considered related to product
  • Grade 3 and worse solicited clinical and laboratory adverse events [ Time Frame: within 7 days of receiving vaccine injection ]
    Grade 3 and worse solicited clinical and laboratory adverse events
  • Serious adverse events [ Time Frame: week 0-74 ]
    Serious adverse events
  • Other clinical and laboratory adverse events [ Time Frame: week 0-74 ]
    Other clinical and laboratory adverse events
  • Binding antibodies [ Time Frame: week 0-74 ]
    Binding antibodies to Cn54gp140 and AIDSVAX® B/E gp120
  • Resistance mutations to tenofovir and emtricitabine [ Time Frame: week 0-74 ]
    Genotypic resistance at HIV seroconversion, focussing on the mutations selected by tenofovir and emtricitabine (codons 65, 70, 184 in reverse transcriptase)
  • Number of PrEP pills missed [ Time Frame: week 0-26 ]
    Adherence to PrEP assessed by self-report
  • Tenofovir level in urine [ Time Frame: week 0-26 ]
    Adherence to PrEP assessed by results of point of care urine tests
  • Tenofovir level in red blood cells [ Time Frame: week 0-26 ]
    Adherence assessed by TFV DP levels measured on DBS in red blood cells
  • Number of PrEP Pills dispensed [ Time Frame: week 0-26 ]
    Adherence assessed by total number of PrEP pills dispensed
Original Secondary Outcome Measures  ICMJE Same as current
Current Other Pre-specified Outcome Measures Not Provided
Original Other Pre-specified Outcome Measures Not Provided
 
Descriptive Information
Brief Title  ICMJE A Combination Efficacy Study in Africa of Two DNA-MVA-Env Protein or DNA-Env Protein HIV-1 Vaccine Regimens With PrEP
Official Title  ICMJE A Phase IIb Three-arm, Two-stage HIV Prophylactic Vaccine Trial With a Second Randomisation to Compare TAF/FTC to TDF/FTC as Pre-exposure Prophylaxis
Brief Summary

This international, multi-centre, double-blind vaccine study is a three-arm prospective 1:1:1 randomisation comparing each of two experimental combination vaccine regimens i.e. DNA/AIDSVAX (weeks 0,4,24,48) and DNA/CN54gp140 (weeks 0,4) + MVA/CN54gp140 (weeks 24,48) with placebo control. There will be a concurrent open-label 1:1 randomisation to compare daily TAF/FTC (week 0-26) to daily TDF/FTC (weeks 0-26) as pre-exposure prophylaxis.

The study aims to randomise up to 1668 eligible adults (18-40 years) through collaborating clinical research centres in 4 countries (Mozambique; South Africa; Tanzania; and Uganda). Each participant will be followed for a minimum of 74 weeks after enrolment.

The trial is designed to detect a reduction in HIV incidence that has public health relevance sufficient to justify implementation of the combination vaccine regimen. In light of the high level of effectiveness demonstrated in the PrEP trials (up to 86% reduction in HIV), this trial is powered to detect a protective vaccine efficacy of 70% at the final analysis.

The PrEP component will determine whether the effectiveness of TAF/FTC is unacceptably lower than the effectiveness of TDF/FTC.

Detailed Description

This international, multi-centre, double-blind vaccine study will be a three-arm prospective 1:1:1 randomisation comparing each of two experimental combination vaccine regimens with placebo control.

Pre-screening for risk and HIV status will take place as part of a Registration Cohort which will precede and continue in parallel to the PrEPVacc trial enrolments. This will give HIV negative volunteers time to learn about the PrEPVacc trial and facilitate timely enrolment.

Clinical screening for the vaccine trial will take place during the 8 weeks prior to randomisation from local communities in Mozambique, South Africa, Tanzania and Uganda where the clinical research centres are located. Eligible participants who are HIV-uninfected adults aged 18-40 years at high risk of HIV infection will be enrolled at week 0 and randomised to one of three vaccine arms:

  1. Vaccine group A: DNA-HIV-PT123 and AIDSVAX® B/E (weeks 0,4,24,48)
  2. Vaccine group B: DNA-HIV-PT123 and CN54gp140 in MPLA-L (wks 0,4), then MVA-CMDR and CN54gp140 in MPLA-L (wks 24,48)
  3. Vaccine group C: Saline Placebo (wks 0,4,24,48)

There will be a concurrent open-label 1:1 randomisation to one of two PrEP regimens:

  1. Control PrEP: Daily TDF/FTC (week 0-26)
  2. Experimental PrEP: Daily TAF/FTC (week 0-26)

Participants will be randomised at each clinical centre through web randomisation after entering the quantifiable eligibility criteria. Randomisation will be stratified by centre and by gender for vaccines and for PrEP. Clinic staff and participants will be blind to allocation of active or placebo vaccines, but the pharmacist preparing the vaccines will know. As the volume of gp140 in MPLA-L is 0.4ml and given at the same timepoints as products with a volume of 1ml, clinic staff will be able to identify participants allocated to the CN54gp140 in MPLA-L or matched placebo.

Clinic staff and participants will know which PrEP agent each participant is allocated to. Participants will continue to receive study PrEP through to week 26 after which access to PrEP will revert to local supply of generic drug.

The target accrual is around 1668 HIV uninfected adults, but this is an endpoint driven multi-arm, multi-stage (MAMS) trial design, and therefore the target may be adjusted following a recommendation from the IDMC. In addition, participants who do not complete the third immunisation will be replaced whilst this is feasible. Participants will be followed up for a minimum of 74 weeks after enrolment.

The primary efficacy outcome measure for the vaccine analysis is HIV acquisition by a participant who completed three immunisations and was HIV negative at week 26.

The primary efficacy outcome for the PrEP analysis is HIV acquisition at or before week 26 by a participant who was HIV negative at enrolment.

The primary safety outcome for both analyses is a clinical decision to discontinue the vaccine or PrEP regimen for an adverse event that is considered related to product.

This trial is designed to detect a reduction in HIV incidence that has public health relevance sufficient to justify implementation of the combination vaccine regimen. In light of the high level of effectiveness demonstrated in the PrEP trials (up to 86% reduction in HIV), this trial is powered to detect a protective vaccine efficacy of 70% at the final analysis.

The PrEP component of the trial aims to show the effectiveness of TAF/FTC is not unacceptably lower than the effectiveness of TDF/FTC, assessed from the observed lower confidence limit for the Averted Infections Ratio (AIR).

The Independent Data Monitoring Committee will review an interim analysis of vaccine efficacy in order to determine whether each active vaccine arm has demonstrated sufficient efficacy to warrant further investigation. This analysis will only consider new infections arising after the week 26 visit and only those in individuals who have completed the first three immunisations. The analysis will take place after approximately 7 of these infections have occurred in the placebo group. The investigators will not be informed of the timing of the interim analysis, unless there is a recommendation to modify the protocol.

The PrEP analysis will consider new infections up to the week 26 visit in individuals who were HIV negative at enrolment.

Study Type  ICMJE Interventional
Study Phase  ICMJE Phase 2
Study Design  ICMJE Allocation: Randomized
Intervention Model: Parallel Assignment
Intervention Model Description:

Group A: DNA-HIV-PT123/AIDSVAX B/E®/TDF/FTC (Truvada) once daily (wks 0-26) Group B: DNA-HIV-PT123 and CN54gp140+MPLA-L (wks 0,4), then MVA-CMDR and CN54gp140+MPLA-L/ TDF/FTC(Truvada) once daily (wks 0-26) Group C: Saline placebo (wks 0,4,24,48)/ TDF/FTC (Truvada) once daily (wks 0-26) Group D: DNA-HIV-PT123/AIDSVAX B/E®/ TAF/FTC (Descovy) once daily (wks 0-26) Group E: DNA-HIV-PT123 and CN54gp140+MPLA-L (wks 0,4), then MVA-CMDR and CN54gp140+MPLA-L/ TAF/FTC (Descovy) once daily (wks 0-26) Group F: Saline placebo (wks 0,4,24,48)/ TAF/FTC (Descovy) once daily (wks 0-26)

Group G: Saline placebo (wks 0,4,24,48)/ TAF/FTC (Descovy) once daily (wks 0-26)

Masking: Triple (Care Provider, Investigator, Outcomes Assessor)
Masking Description:

The vaccine component of PrEPVacc is placebo-controlled. Study staff, participants, laboratory staff and clinical staff assessing safety outcomes will not know who has been allocated vaccine or placebo, but pharmacy staff will know. The committee assessing the efficacy endpoints will not see the allocation in the first instance. The IDMC and statistical staff preparing the closed reports for the IDMC will also know the allocation.

Clinic staff will see the difference in volume between CN54gp140 in MPLA-L/matched placebo (0.4ml) and DNA-HIV-PT123/matched placebo (1ml) due to the position of the plunger, but they will not be able to differentiate between active and placebo.

The randomisation to control PrEP: experimental PrEP is 1:1 and all study staff and participants will know the allocation after randomisation as this is open-label.

Primary Purpose: Prevention
Condition  ICMJE HIV Infections
Intervention  ICMJE
  • Biological: Vaccine Group A: DNA-HIV-PT123 and AIDSVAX® B/E (weeks 0,4,24,48)
    1. DNA-HIV-PT123 HIV vaccine includes three DNA plasmids that encode clade C ZM96 Gag, clade C ZM96 Env, and CN54 Pol-Nef.
    2. AIDSVAX® B/E is a bivalent HIV gp120 glycoprotein encompassing both subtype B (MN) and subtype E (A244) proteins that are adsorbed onto 600mcg of aluminum hydroxide gel suspension as adjuvant.
  • Biological: Vaccine Group B: DNA-HIV-PT123 and CN54gp140+MPLA-L (weeks 0,4), then MVA and CN54gp140+MPLA-L (weeks 24,48)
    1. DNA-HIV-PT123 (see above)
    2. CN54gp140+MPLA-L. Recombinant CN54gp140 is a HIV-1 envelope protein from the clade C strain 97/CN/54 isolate, which comprises a sequence of 634 amino acids. MPLA is a non-toxic version of LipoPolySaccharide (LPS), which is isolated from the LPS lipid A region of Salmonella Minnesota R595 and retains the immune-stimulatory properties of LPS, but exhibits low toxicity.
    3. MVA-CMDR (Modified Vaccinia Ankara-Chiang Mai Double Recombinant) is a non-replicating, highly attenuated strain of Vaccina virus that has been genetically engineered to express the HIV-1 genes envgp160 CM235 Subtype E and gag and pol CM240 Subtype A (integrase-deleted and reverse transcriptase non-functional).
  • Biological: Vaccine Group C: Saline placebo (weeks 0,4,24,48)
    Sodium Chloride (NaCl) for injection, 0.9%
  • Drug: Control PrEP:TDF/FTC once daily (weeks 0-26)
    Each tablet of Truvada contains 245mg of tenofovir disoproxil (TDF) and 200mg of emtricitabine (FTC), both of which are nucleot/side analogue HIV-1 reverse transcriptase inhibitors.
    Other Name: Truvada
  • Drug: Experimental PrEP:TAF/FTC once daily (weeks 0-26)
    Each tablet of Descovy contains 25mg of tenofovir alfenamide (TAF) and 200mg of emtricitabine (FTC), both of which are nucleot/side analogue HIV-1 reverse transcriptase inhibitors.
    Other Name: Descovy
Study Arms  ICMJE
  • Experimental: Group A

    278 participants will receive DNA-HIV-PT123 vaccine and AIDSVAX® B/E protein at weeks 0, 4, 24 and 48.

    1ml of DNA-HIV-PT123 will be injected into the deltoid muscle of the left upper arm

    1ml of AIDSVAX® B/E will be injected into the deltoid muscle of the right arm

    1 tab of Truvada (0-26 weeks)

    Interventions:
    • Biological: Vaccine Group A: DNA-HIV-PT123 and AIDSVAX® B/E (weeks 0,4,24,48)
    • Drug: Control PrEP:TDF/FTC once daily (weeks 0-26)
  • Experimental: Group B

    278 participants will receive DNA-HIV-PT123 and CN54gp140+MPLA-L at weeks 0 and 4, then MVA and CN54gp140+MPLA-L at weeks 24 and 48

    1ml of DNA-HIV-PT123 will be injected into the deltoid muscle of the left upper arm

    1ml (1x108 pfu) of MVA will be injected into the deltoid muscle of the left upper arm

    0.4mL containing a mixture of 100mcg CN54gp140 and 5mcg MPLA-L will be injected into the deltoid muscle of the right upper arm

    1 tab of Truvada (0-26 weeks)

    Interventions:
    • Biological: Vaccine Group B: DNA-HIV-PT123 and CN54gp140+MPLA-L (weeks 0,4), then MVA and CN54gp140+MPLA-L (weeks 24,48)
    • Drug: Control PrEP:TDF/FTC once daily (weeks 0-26)
  • Placebo Comparator: Group C:

    278 participants will receive Sodium Chloride 0.9% (Normal Saline) placebo at weeks 0,4,24, and 48

    The volume will be matched to the vaccine at 1ml for DNA, MVA and AIDSVAX® B/E, but 0.4ml for CN54gp140 in MPLA-L. Participants will be randomly divided in a 1:1 ratio to receive 1ml in each arm at the four timepoints or 1ml in the left arm and 0.4ml in the right arm at the four timepoints.

    1 tab of Truvada (0-26 weeks)

    Interventions:
    • Biological: Vaccine Group C: Saline placebo (weeks 0,4,24,48)
    • Drug: Control PrEP:TDF/FTC once daily (weeks 0-26)
  • Active Comparator: Group D

    278 participants will receive DNA-HIV-PT123 vaccine and AIDSVAX® B/E protein at weeks 0, 4, 24 and 48.

    1ml of DNA-HIV-PT123 will be injected into the deltoid muscle of the left upper arm

    1ml of AIDSVAX® B/E will be injected into the deltoid muscle of the right arm

    1 tab of Descovy (0-26 weeks)

    Interventions:
    • Biological: Vaccine Group A: DNA-HIV-PT123 and AIDSVAX® B/E (weeks 0,4,24,48)
    • Drug: Experimental PrEP:TAF/FTC once daily (weeks 0-26)
  • Experimental: Group E

    278 participants will receive DNA-HIV-PT123 and CN54gp140+MPLA-L at weeks 0 and 4, then MVA and CN54gp140+MPLA-L at weeks 24 and 48

    1ml of DNA-HIV-PT123 will be injected into the deltoid muscle of the left upper arm

    1ml (1x108 pfu) of MVA will be injected into the deltoid muscle of the left upper arm

    0.4mL containing a mixture of 100mcg CN54gp140 and 5mcg MPLA-L will be injected into the deltoid muscle of the right upper arm

    1 tab of Descovy (0-26 weeks)

    Interventions:
    • Biological: Vaccine Group B: DNA-HIV-PT123 and CN54gp140+MPLA-L (weeks 0,4), then MVA and CN54gp140+MPLA-L (weeks 24,48)
    • Drug: Experimental PrEP:TAF/FTC once daily (weeks 0-26)
  • Placebo Comparator: Group G

    278 participants will receive Sodium Chloride 0.9% (Normal Saline) placebo at weeks 0,4,24, and 48

    The volume will be matched to the vaccine at 1ml for DNA, MVA and AIDSVAX® B/E, but 0.4ml for CN54gp140 in MPLA-L. Participants will be randomly divided in a 1:1 ratio to receive 1ml in each arm at the four timepoints or 1ml in the left arm and 0.4ml in the right arm at the four timepoints.

    1 tab of Descovy (0-26 weeks)

    Interventions:
    • Biological: Vaccine Group C: Saline placebo (weeks 0,4,24,48)
    • Drug: Experimental PrEP:TAF/FTC once daily (weeks 0-26)
Publications * Not Provided

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Recruitment Information
Recruitment Status  ICMJE Not yet recruiting
Estimated Enrollment  ICMJE
 (submitted: August 21, 2019)
1668
Original Estimated Enrollment  ICMJE Same as current
Estimated Study Completion Date  ICMJE March 2023
Estimated Primary Completion Date March 2023   (Final data collection date for primary outcome measure)
Eligibility Criteria  ICMJE

Inclusion criteria

  1. HIV uninfected adults aged between 18 and 40 years old on the day of screening
  2. Willing and able to provide informed consent prior to participation
  3. Willing and able to comply with the visit schedule and provide blood, urine and other samples at the required time points
  4. Home address accessible for visiting and intending to remain within the recruitment area for at least 82 weeks from screening
  5. Likely to be at risk from exposure to HIV during follow up
  6. Willing to undergo HIV testing, receive HIV test results and risk reduction counselling which includes promotion of PrEP and condoms
  7. If female, of child-bearing age and not sterilised, willing to use a highly effective method of contraception from screening until 18 weeks after the last injection
  8. If male and not sterilised, willing to avoid impregnating female partners from screening until 18 weeks after the last injection

Exclusion criteria

  1. HIV infection or indeterminate HIV result at screening or enrolment
  2. Hepatitis B surface antigen positive
  3. If female, currently pregnant (evidence from positive serum or urine pregnancy test), or lactating
  4. Participating in another biomedical research study or in receipt of a live vaccine within 30 days prior to randomisation
  5. Participation in a previous HIV vaccine or HIV immunotherapy trial
  6. Receiving blood products or immunoglobulins within 12 weeks of screening
  7. Known hypersensitivity to any component of the vaccine formulations used in this trial or history of severe or multiple allergies to vaccines, drugs or pharmaceutical agents
  8. Presence of a systemic disease at the time of randomisation or history of chronic illness that in the opinion of the investigator may compromise the participant's safety, preclude vaccination or compromise an immune response to vaccine
  9. Abnormalities in routine laboratory parameters (Hb, creatinine, AST/ALT, alkaline phosphatase, total Bilirubin and glucose) of Grade 2 and above using the DAIDS toxicity table, version 2.1 July 2017 or estimated glomerular filtration rate less than 50ml/min
Sex/Gender  ICMJE
Sexes Eligible for Study: All
Ages  ICMJE 18 Years to 40 Years   (Adult)
Accepts Healthy Volunteers  ICMJE Yes
Contacts  ICMJE
Contact: Bernadette K Nayiga, MSc +25641770400 ext 187 Berna.kalanzi@mrcuganda.org
Contact: Eugene Ruzagira, PhD +256417704000 Eugene.ruzagira@mrcuganda.org
Listed Location Countries  ICMJE Uganda
Removed Location Countries  
 
Administrative Information
NCT Number  ICMJE NCT04066881
Other Study ID Numbers  ICMJE PV1
Has Data Monitoring Committee Yes
U.S. FDA-regulated Product
Studies a U.S. FDA-regulated Drug Product: Yes
Studies a U.S. FDA-regulated Device Product: No
Product Manufactured in and Exported from the U.S.: Yes
IPD Sharing Statement  ICMJE
Plan to Share IPD: Yes
Plan Description: The investigators will ensure that optimal use is made of the data generated in this trial through a controlled access approach to data sharing. Access will be controlled to ensure that there is a scientific rationale for the data, that no data are released that could compromise the ongoing trial, that the appropriate consent is in place, that an appropriate agreement is in place for secure transfer and storage, and that resources required to process data release are adequate.
Supporting Materials: Study Protocol
Supporting Materials: Statistical Analysis Plan (SAP)
Supporting Materials: Informed Consent Form (ICF)
Supporting Materials: Clinical Study Report (CSR)
Time Frame:

The approved versions of the study protocol and global informed consent form will be in the public domain throughout.

The Statistical Analysis Plan will be in the public domain prior to database lock.

The clinical study report will be available a year after the last participant visit.

Access Criteria:

The approved protocols and final version of the SAP will be in the public domain.

The clinical study report will be available on request.

Responsible Party Prof Pontiano Kaleebu, MRC/UVRI and LSHTM Uganda Research Unit
Study Sponsor  ICMJE MRC/UVRI and LSHTM Uganda Research Unit
Collaborators  ICMJE
  • Imperial College London
  • University College, London
  • International AIDS Vaccine Initiative
  • EuroVacc Foundation
  • Medical Research Council, South Africa
  • National Institute for Medical Research, Tanzania
  • Muhimbili University of Health and Allied Sciences
  • Instituto Nacional de Saúde, Mozambique
  • Ludwig-Maximilians - University of Munich
  • King's College London
  • Centre Hospitalier Universitaire Vaudois
  • Karolinska Institutet
  • CONRAD
  • Gilead Sciences
Investigators  ICMJE
Principal Investigator: Pontiano Kaleebu, PhD MRC/UVRI and LSHTM Uganda Resae
Study Chair: Sheena McCormack, MSc MRC CTU at UCL
Study Director: Jonathan Weber, PhD Imperial College London
PRS Account MRC/UVRI and LSHTM Uganda Research Unit
Verification Date August 2019

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP