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Identification of Epigenetic Biomarkers Allowing to Predict the Evolution of Lung Disease Severity in Cystic Fibrosis, in Order to Improve Patient Follow-up and to Move Toward Personalized Medicine (MethylBiomark)

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ClinicalTrials.gov Identifier: NCT02976714
Recruitment Status : Recruiting
First Posted : November 29, 2016
Last Update Posted : May 12, 2017
Sponsor:
Information provided by (Responsible Party):
University Hospital, Montpellier

November 8, 2016
November 29, 2016
May 12, 2017
December 12, 2016
November 2018   (Final data collection date for primary outcome measure)
  • DNA methylation [ Time Frame: at time of inclusion ]
    Spontaneous expectoration at baseline
  • DNA methylation [ Time Frame: 6 months ]
    Spontaneous expectoration at 6 months
  • DNA methylation [ Time Frame: 12 months ]
    Spontaneous expectoration at 12 months
  • DNA methylation [ Time Frame: 18 months ]
    Spontaneous expectoration at 18 months
Same as current
Complete list of historical versions of study NCT02976714 on ClinicalTrials.gov Archive Site
Not Provided
Not Provided
Not Provided
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Identification of Epigenetic Biomarkers Allowing to Predict the Evolution of Lung Disease Severity in Cystic Fibrosis, in Order to Improve Patient Follow-up and to Move Toward Personalized Medicine
Identification of Predictive Epigenetic Biomarkers of Lung Disease Severity in Cystic Fibrosis
The general aims of this project are (i) to identify predictive epigenetic biomarkers of lung disease severity in Cystic Fibrosis, (ii) to characterize a non-invasive cellular model, spontaneous sputum, for the analysis of these epigenetic biomarkers, (iii) to analyze the variations in DNA methylation for a same patient over time.

Cystic Fibrosis (CF) is an autosomal recessive disease resulting from mutations in the CFTR gene. CFTR encodes a chloride channel, mainly expressed at the apical membrane of epithelial cells. CFTR dysfunction induces mucus thickening, causing multiple disorders in respiratory, digestive and reproductive tracts. In CF patients, lung disease is the main cause of morbidity and mortality, and its severity is variable among CF patients, even among those with the same genotype. This clinical variability depends on two factors: genetic (complex alleles of CFTR gene and modifier genes) and environmental factors. Genetic factors have been largely explored, and several modifier genes have been identified. By contrast, environmental factors are still poorly known. It is well established that environmental factors modify the phenotype by acting on epigenetic marks (i.e. DNA methylation, histone modification) present in the genome. Epigenetic modifications regulate and modulate gene expression.

In a previous we profiled DNA methylation genome-wide in nasal epithelial cell samples from 32 CF patients and 24 healthy controls. CF patients homozygous for the p.Phe508del mutation and >18-years-old were stratified according to the lung disease severity. Through this study, we identified 187 genomic regions (CpG dinucleotides) differentially methylated between CF patients with mild lung disease and CF patients with severe lung disease. The present project aims at identifying predictive epigenetic biomarkers of lung disease severity, among these 187 regions. While the previous study was carried out on genomic DNA extracted from nasal epithelial cells, in the present project we will use a non-invasive model: spontaneous sputum.

Hypothesis: some differentially methylated genomic regions between mild and severe CF patients can be used as predictive epigenetic biomarkers of lung disease severity in cystic fibrosis.

Objectives: (i) to identify predictive epigenetic biomarkers of lung disease severity among the differentially methylated genomic regions between mild and severe CF patients, (ii) to characterize a non-invasive cellular model, spontaneous sputum, for the analysis of epigenetic biomarkers of lung disease severity in CF, (iii) to analyze the variations in DNA methylation for a same patient over time (at time of inclusion, 6 months, 12 months and 18 months)

Interventional
Not Applicable
Intervention Model: Single Group Assignment
Masking: None (Open Label)
Primary Purpose: Prevention
Cystic Fibrosis
Other: spontaneous sputum

CF patients carry a spontaneous sputum that is made in the context of bronchial drainage sessions conducted as part of usual care (inclusion, 6 months, 12 months, 18 months).

The collection of spontaneous sputum is carried out within the bronchial drainage sessions, which are routinely performed at each visit. The spontaneous sputum is collected in a sterile container. In order not to contaminate the sputum with buccal epithelial cells will be asked patients to rinse the mouth before expectorate.

CF patients
CF patients carry a spontaneous sputum that is made in the context of bronchial drainage sessions conducted as part of usual care.
Intervention: Other: spontaneous sputum
Not Provided

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Recruiting
50
Same as current
May 2019
November 2018   (Final data collection date for primary outcome measure)

Inclusion Criteria:

  • free and informed consent obtained, and consent signed
  • subject covered by a state insurance scheme
  • women and men aged 12 to 30
  • subject affected by cystic fibrosis, carrier of two severe mutations in trans (in the same allele) in the CFTR gene
  • subject able to realize a spirometry before and during the study

Exclusion Criteria:

  • subject in an exclusion period of a previous study
  • subject placed under judicial protection, guardianship or supervision
  • impossibility to give informed information to the subject
  • subject who does not read fluently French
  • pregnancy
  • breastfeeding
  • transplanted subject
Sexes Eligible for Study: All
12 Years to 30 Years   (Child, Adult)
No
Contact: Albertina De Sario, PhD 4 11 75 98 63 ext 33 albertina.de-sario@inserm.fr
Contact: Davide CAIMMI, MD
France
 
 
NCT02976714
UF9745
Yes
Not Provided
Plan to Share IPD: Undecided
University Hospital, Montpellier
University Hospital, Montpellier
Not Provided
Principal Investigator: Davide CAIMMI University Hospital, Montpellier
University Hospital, Montpellier
November 2016

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP