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Trial record 1 of 1 for:    NCT02125682
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The HDL Particle Protection Study (HDL PROTECT)

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT02125682
Recruitment Status : Completed
First Posted : April 29, 2014
Last Update Posted : February 1, 2018
Nouvelle Société Française d'Athérosclérose
Information provided by (Responsible Party):
Rabih Azar, Hotel Dieu de France Hospital

Tracking Information
First Submitted Date  ICMJE April 27, 2014
First Posted Date  ICMJE April 29, 2014
Last Update Posted Date February 1, 2018
Study Start Date  ICMJE January 2012
Actual Primary Completion Date May 14, 2017   (Final data collection date for primary outcome measure)
Current Primary Outcome Measures  ICMJE
 (submitted: April 28, 2014)
effect on HDL function [ Time Frame: 8 weeks ]
Does atorvastatin 80 mg/day improve HDL function more than 10 mg/day. HDL function will be assessed via several tests
Original Primary Outcome Measures  ICMJE Same as current
Change History
Current Secondary Outcome Measures  ICMJE Not Provided
Original Secondary Outcome Measures  ICMJE Not Provided
Current Other Pre-specified Outcome Measures Not Provided
Original Other Pre-specified Outcome Measures Not Provided
Descriptive Information
Brief Title  ICMJE The HDL Particle Protection Study
Official Title  ICMJE Atorvastatin Action on Oxidative Stress and Inflammation in Type II Diabetes: The HDL Particle Protection Study
Brief Summary Atorvastatin is a statin that significantly decreases LDL level. At 10 mg/day, atorvastatin increases HDL level by 4-5%. At 80 mg/day, atorvastatin does not increase HDL level. However, atorvastatin is more protective at 80 mg/day than at 10 mg/day. This is due to a better reduction in LDL level at 80 mg, but we also think that 80 mg/day of atorvastatin is superior to 10 mg/day in improving the QUALITY of HDL, such as improving HDL particle number and function (better anti-oxydant activity)
Detailed Description

The dyslipidemia of Type II diabetes is characterized by anomalies of the metabolism and biological activities of both atherogenic lipoproteins containing apoB100 (VLDL, IDL and LDL) and of antiatherogenic HDL containing apoAI and/or apoAII. Such metabolic and functional anomalies are closely associated with elevated oxidative stress, endothelial dysfunction and premature macrovascular atherosclerotic disease. The ratio of atherogenic cholesterol (VLDL, IDL, LDL cholesterol) relative to HDL cholesterol (HDL-C) in normolipidemic subjects is typically less than 3; by contrast, ratios of 4 or more are typical of the dyslipidemia of Type II diabetes and are indicative of disequilibrium in proatherogenic versus antiatherogenic plasma lipoprotein levels, frequently due to low HDL-C concentration (<40 mg/dl). Such conditions favor enhanced deposition of cholesterol in the arterial wall and progression of atherosclerotic disease.

Atorvastatin is a potent synthetic HMG-CoA reductase inhibitor which markedly lowers plasma levels of LDL cholesterol (LDL-C); in addition, atorvastatin lowers plasma levels of triglycerides (TG) and TG-rich lipoproteins but equally raises levels of HDL-C and apoAI, the major HDL apolipoprotein. Atorvastatin-induced decrease in plasma TG is intimately related to decreased VLDL levels, accelerated VLDL turnover and normalized intravascular remodeling of apoB-containing lipoproteins. Importantly, atorvastatin reduces activities of plasma cholesteryl ester transfer protein (CETP) and hepatic lipase (HL), thereby leading to the normalized remodeling of both LDL and HDL particle populations. Furthermore, recent studies have revealed that in atherogenic Type IIB hyperlipidemia, atorvastatin induces a dose-dependent and progressive increase in the capacity of both plasma and HDL to mediate cellular cholesterol efflux via the SRB1 receptor pathway.

Plasma HDL is highly heterogeneous. When isolated on the basis of density by ultracentrifugation, human HDL is separated into two major subfractions, large, light HDL2 and small, dense HDL3. HDL remodeling by CETP, HL and LCAT can alter absolute and relative concentrations of HDL2 and HDL3 in plasma. It remains contradictory however as to whether plasma levels of HDL2 or HDL3 are predictors of cardiovascular risk. HDL exerts a spectrum of antiatherosclerotic actions; central among them are reverse cholesterol transport, the capacity of HDL to protect LDL against oxidative stress, the anti-inflammatory actions of HDL on arterial wall cells as well as antithrombotic activities. We have recently found that small, dense HDL3 particles exert potent protection of atherogenic LDL subspecies against oxidative stress in normolipidemic subjects and that HDL-associated paraoxonase (PON) 1, platelet-activating factor acetylhydrolase (PAF-AH) and lecithin:cholesterol acyltransferase (LCAT) activities can contribute to such antioxidative properties. HDL particles are however dysfunctional in diabetic dyslipidemias; for example, diabetic HDL are deficient in antioxidant activity, and in addition, their cholesterol-efflux capacity is impaired. Such dysfunction may lead to impairment of the antiatherogenic actions of HDL in diabetic dyslipidemia.

Working hypothesis:

The investigators hypothesize that atorvastatin can increase plasma levels of HDL subfractions with potent antioxidant activity as a result of enhanced surface and core remodeling of TG-rich lipoproteins, (such as VLDL-1 and VLDL-2), reduced CETP activity, and stimulation of apoAI production. Indeed, Asztalos et al. showed that atorvastatin induced significant increase in the α1, α2, pre- α1 and pre-β1 HDL subfractions in dyslipidemic subjects with mean LDL-C, 198 mg/dl; mean TG, 167 mg/dl.

Study Type  ICMJE Interventional
Study Phase  ICMJE Phase 4
Study Design  ICMJE Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Single (Outcomes Assessor)
Primary Purpose: Treatment
Condition  ICMJE Dyslipidemia in Patients With Diabetes Mellitus
Intervention  ICMJE
  • Drug: atovastatin 10 mg/day
    patients will receive 10 mg of atorvastatin daily for 8 weeks, then wash out for 6 weeks then cross over to atorvastatin 80 mg daily for 8 weeks
  • Drug: Atorvastatin 80 mg/day
    patients will receive 80 mg of atorvastatin daily for 8 weeks, then wash out for 6 weeks then cross over to atorvastatin 10 mg daily for 8 weeks
Study Arms  ICMJE
  • Active Comparator: low dose
    patients receiving 10 mg of atorvastatin daily
    Intervention: Drug: atovastatin 10 mg/day
  • Active Comparator: High dose
    Patients receiving 80 mg of atorvastatin daily
    Intervention: Drug: Atorvastatin 80 mg/day
Publications * Not Provided

*   Includes publications given by the data provider as well as publications identified by Identifier (NCT Number) in Medline.
Recruitment Information
Recruitment Status  ICMJE Completed
Actual Enrollment  ICMJE
 (submitted: April 28, 2014)
Original Estimated Enrollment  ICMJE Same as current
Actual Study Completion Date  ICMJE May 14, 2017
Actual Primary Completion Date May 14, 2017   (Final data collection date for primary outcome measure)
Eligibility Criteria  ICMJE

Inclusion Criteria: patient should have all of the 3 criteria:

  1. Patient with diabetes mellitus, defined by at least 1 of the following:

    Fasting glucose > 125 mg/dL confirmed on 2 occasions HbA1C > 6.5% Patients receiving any glucose lowering agent (oral or subcutaneous)

  2. Lipid profile should have ALL of the following characteristics:

    Triglycerides >150 mg/dL HDL <45 mg/dL LDL < 190 mg/dL

  3. Lp(a) level < 30 mg/dL

Exclusion Criteria:

  1. Patients with known coronary artery disease defined by at least one of the following:

    • Prior myocardial infarction
    • Prior PCI
    • Prior CABG
    • Known coronary stenosis > 50% on coronary angiography
    • A non invasive study revealing myocardial ischemia (such as a stress test, a nuclear perfusion study or a stress echo)
  2. Poor diabetic control defined by an HbA1c > 8.5% in the preceding 3 months
  3. Patients with known diabetic retinopathy, nephropathy or neuropathy
  4. Patients with a creatinin clearance < 75 ml/min as calculated by the Cockcroft-Gault equation
  5. Patients who have received any lipid lowering therapy within 6 weeks prior to inclusion (statin, fibrates, ezetimibe, niacin, resin binding agent)
  6. Patients with underlying malignancy or infection or inflammatory disease
  7. Patients with SGPT or SGOT or CK > 2.5 times upper reference value
  8. Patients allergic to statins or who experienced prior significant side effects with statins such as elevation of liver enzymes or CK > 2.5 upper reference value
  9. Patients older than 80
  10. Females who are premenopausal
  11. Patients unable to give informed consent
Sex/Gender  ICMJE
Sexes Eligible for Study: All
Ages  ICMJE 21 Years to 80 Years   (Adult, Older Adult)
Accepts Healthy Volunteers  ICMJE Not Provided
Contacts  ICMJE Contact information is only displayed when the study is recruiting subjects
Listed Location Countries  ICMJE Lebanon
Removed Location Countries  
Administrative Information
NCT Number  ICMJE NCT02125682
Other Study ID Numbers  ICMJE HDLPROTECT
Has Data Monitoring Committee Yes
U.S. FDA-regulated Product Not Provided
IPD Sharing Statement  ICMJE Not Provided
Responsible Party Rabih Azar, Hotel Dieu de France Hospital
Study Sponsor  ICMJE Hotel Dieu de France Hospital
Collaborators  ICMJE
  • Nouvelle Société Française d'Athérosclérose
  • Pfizer
Investigators  ICMJE
Study Chair: M. John Chapman, PhD INSERM Pitié Salpetriere, Paris, France
PRS Account Hotel Dieu de France Hospital
Verification Date January 2018

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP