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Comparison of Bacterial Contamination Rates Between Isolation and Non-isolation Rooms

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ClinicalTrials.gov Identifier: NCT01671358
Recruitment Status : Completed
First Posted : August 23, 2012
Last Update Posted : June 2, 2015
Sponsor:
Information provided by (Responsible Party):
Michael Sweet, West Virginia University

August 16, 2012
August 23, 2012
June 2, 2015
August 2012
June 2013   (Final data collection date for primary outcome measure)
Presence of MRSA colonization [ Time Frame: Hospital stay, an expected average of 14 days ]
Swabbing of four areas of the medication cabinet will be performed (keypad, handle, medication folder and a medication). A Semi-quantitative sampling technique will be used to collect the samples. A sterile saline moistened sterile cotton tip swab will be used to swab the specified areas. The swabs will then be used to inoculate sheep blood agar plates in 4 quadrants. The plates will be incubated for 48 hours. The number of quadrants with growth will be identified. Individual colonies will be removed from the plate with an inoculation loop, then streaked onto a second sheep blood agar plate. The second plates will be incubated for 48 hours. The colonies will then be run through standard identification tests to determine if the colonies are MRSA or not. A comparison will be made between non-isolation and isolation rooms.
Same as current
Complete list of historical versions of study NCT01671358 on ClinicalTrials.gov Archive Site
Quantification of MRSA colonization [ Time Frame: Hospital stay, an expected average of 14 days ]
Swabbing of four areas of the medication cabinet will be performed (keypad, handle, medication folder and a medication). A Semi-quantitative sampling technique will be used to collect the samples. A sterile saline moistened sterile cotton tip swab will be used to swab the specified areas. The swabs will then be used to inoculate sheep blood agar plates in 4 quadrants. The plates will be incubated for 48 hours. The number of quadrants with growth will be identified. A comparison between the sites of swabbing will be performed.
Same as current
  • Presence of other bacterial colonization [ Time Frame: Hospital stay, an expected average of 14 days ]
    Swabbing of four areas of the medication cabinet will be performed (keypad, handle, medication folder and a medication). A Semi-quantitative sampling technique will be used to collect the samples. A sterile saline moistened sterile cotton tip swab will be used to swab the specified areas. The swabs will then be used to inoculate sheep blood agar plates in 4 quadrants. The plates will be incubated for 48 hours. The number of quadrants with growth will be identified. Individual colonies will be removed from the plate with an inoculation loop, then streaked onto a second sheep blood agar plate. The second plates will be incubated for 48 hours. The colonies will be categorized as gram negative, gram positive catalase negative, Gram positive coagulase negative Staphylococcus species or methicillin-sensitive Staphylococcus aureus. This will serve as a baseline epidemiology of the hospital and generate other possible hypotheses.
  • Correlation between MRSA and active infection or colonization of patient [ Time Frame: Hospital stay, an expected average of 14 days ]
    The patient from the same time frame in the rooms sampled and positive for MRSA colonization will be reviewed to determine if they had active infection or were previously colonized.
  • Correlation between colonization of other positive organisms and active infection or colonization of patient [ Time Frame: Hospital stay, an expected average of 14 days ]
    The patient from the same time frame in the rooms sampled and positive for non-MRSA colonization will be reviewed to determine if they had active infection or were previously colonized.
Same as current
 
Comparison of Bacterial Contamination Rates Between Isolation and Non-isolation Rooms
Comparison of Contamination Rates of Medication Storage Cabinets Between Isolation and Non-isolation Rooms With Methicillin-resistant Staphylococcus Aureus (MRSA)
This study is to determine if medication cabinets located outside of isolation rooms in hospitals and their contents, particularly medications and the delivery folders are at a higher risk of having harmful bacteria on them.

Studies show high touch areas maybe contaminated with organisms such as Methicillin—resistant Staphylococcus aureus(MRSA) and vancomycin-resistant enterococci. MRSA can live on hospital surfaces for 9-14 days. Patients in rooms that were previously MRSA isolation rooms are at higher risk for developing a hospital-acquired infection.

This study is to determine if medication cabinets located outside of MRSA isolation rooms and their contents, particularly medications and the pharmacy delivery folders are at a higher risk of having MRSA colonization on them. This study will use conventional methods to determine if MRSA colonization is present and compare results between non-isolation and isolation rooms. This will evaluate if alternate measures for the reduction of MRSA colonization are needed for the MRSA isolation rooms in regards to medication delivery and storage.

Observational
Observational Model: Cohort
Time Perspective: Prospective
Not Provided
Not Provided
Probability Sample
Inaptients at an academic medical center
Methicillin-Resistant Staphylococcus Aureus
Not Provided
  • Isolation Rooms for MRSA
    Rooms that currently have a patient in them that are in isolation status due to MRSA
  • Non-isolation rooms
    Rooms that have not been occupied by a patient in isolation due to MRSA for 14 days

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Completed
400
Same as current
June 2013
June 2013   (Final data collection date for primary outcome measure)

Inclusion Criteria:

  • Patient rooms with isolation status for MRSA
  • Patient rooms without isolation status for MRSA for 14 days

Exclusion Criteria:

  • Rooms without medication cabinets directly outside the room
Sexes Eligible for Study: All
Child, Adult, Senior
Yes
Contact information is only displayed when the study is recruiting subjects
United States
 
 
NCT01671358
WVU-00010
No
Not Provided
Not Provided
Michael Sweet, West Virginia University
West Virginia University
Not Provided
Not Provided
West Virginia University
June 2015