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Primary Cell Culture of Hepatic Tumorous Cells From Routine Fine-needle Aspiration

This study has been completed.
Sponsor:
Collaborator:
Department of Health, Executive Yuan, R.O.C. (Taiwan)
Information provided by (Responsible Party):
Kaohsiung Medical University Chung-Ho Memorial Hospital
ClinicalTrials.gov Identifier:
NCT01549275
First received: February 7, 2012
Last updated: September 7, 2015
Last verified: July 2013

February 7, 2012
September 7, 2015
April 2010
January 2013   (final data collection date for primary outcome measure)
Correlation Between the Growth Speeds of the Cultured Cells and the AJCC TNM Stage (7th Eds) at Entering of the Study. [ Time Frame: 28 days after plating of cells ] [ Designated as safety issue: No ]
Patients were divided into AJCC TNM staging < = IIIA and > = IIIB two groups. The incidences of patients with rapidly proliferative cultured cells in these two groups were compared. Rapidly proliferative group was defined as (1) growth area of cultured cells at the 28th day of primary culture exceeded two times of the growth area measured at the 14th day, or (2) growth area of cultured cells at the 28th day reached > 70% growth area of the flask. Based on the results from special stain, patients in rapidly proliferative group were further divided into patients with rapid proliferation of HCC cells alone, rapid proliferation of HCC cells with concomitant cancer-associated fibroblasts (CAFs) (HCC + CAFs) and CAFs alone.
growth speed of cultured cells [ Time Frame: 28 days after plating of cells ] [ Designated as safety issue: No ]
correlation the growth speed of cells (reach equal or more than 70% growth area of 25 cm2 culture flask) and TNM tumor staging
Complete list of historical versions of study NCT01549275 on ClinicalTrials.gov Archive Site
Correlation Between the Growth Speeds of Cultured Cells and Worsening of AJCC TNM Stages or HCC Related Death 6 Months After Plating of Cells [ Time Frame: 6 months after plating of cells ] [ Designated as safety issue: No ]
104 Patients with complete follow-up data were further divided into receiving (1) curative treatment of HCC including operative resection and local ablation therapy, (2) palliative transcatheter arterial chemoembolization (TACE), and (3) supportive treatment.
  • TNM tumor staging [ Time Frame: 6 months after plating of cells ] [ Designated as safety issue: No ]
    change in tumor staging
  • survival of patient [ Time Frame: 6 months after plating of cells ] [ Designated as safety issue: No ]
    correlation the growth speed of cultured cells measured at 28 days after plating (reach equal or more than 70% growth area of 25 cm2 culture flask)and cancer-related death of patient within 6 months after plating of cells
Not Provided
Not Provided
 
Primary Cell Culture of Hepatic Tumorous Cells From Routine Fine-needle Aspiration
Kaohsiung Medical University Hospital
The purposes of this prospective study were to evaluate the successful rate of primary culture of hepatocellular carcinoma cells and cancer-associated fibroblasts from the residual specimens in routine fine-needle aspiration of hepatic tumor and the potential application of this method as an additional tool for personalized treatment of hepatocellular carcinoma patients.
Fine-needle aspiration (FNA) cytology or biopsy of hepatic tumor is frequently applied in the collection of specimens because it is considered safe, efficacious, accurate and cost effective. Our previous study from small number of patients showed that specimens obtained from FNA had potential to be applied for primary culture of cancer cells. This study was to modified our method for primary culture of both hepatocellular carcinoma cells and cancer-associated fibroblasts in patients with tumor measuring larger than or equal to 3cm. All patients received one session of aspiration for the diagnosis of hepatic tumor. The aspirated specimens were applied for both cytologic and pathologic examinations at first. After then, 10 mL 0.9% NaCl was aspirated by the same needle connected with the same syringe and injected into 15 mL sterilized centrifugation tube. If the tube contained visible specimens, these specimens were sent for primary culture.
Observational
Observational Model: Case-Only
Time Perspective: Prospective
Not Provided
Retention:   Samples With DNA
Description:
cancer cells and cancer-associated fibroblasts
Non-Probability Sample
patients with hepatic tumor measuring larger than or equal to 3 cm underwent fine-needle aspiration of tumor for diagnosis.
Hepatocellular Carcinoma
Not Provided
Not Provided
Lin ZY, Chuang WL, Chuang YH, Yu ML, Hsieh MY, Wang LY, Tsai JF. Discordant influence of amphotericin B on epirubicin cytotoxicity in primary hepatic malignant cells collected by a new primary culture technique. J Gastroenterol Hepatol. 2006 Feb;21(2):398-405.

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Completed
105
July 2013
January 2013   (final data collection date for primary outcome measure)

Inclusion Criteria:

  • patients had residual aspirated specimens for primary culture.

Exclusion Criteria:

  • patients did not have residual aspirated specimens for primary culture.
Both
20 Years to 90 Years   (Adult, Senior)
No
Contact information is only displayed when the study is recruiting subjects
Taiwan
 
NCT01549275
990043
Yes
Not Provided
Not Provided
Kaohsiung Medical University Chung-Ho Memorial Hospital
Kaohsiung Medical University Chung-Ho Memorial Hospital
Department of Health, Executive Yuan, R.O.C. (Taiwan)
Principal Investigator: Zu Y Lin, MD Kaohsiung Medical University
Kaohsiung Medical University Chung-Ho Memorial Hospital
July 2013

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP