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Release of Cytokines by Stimulated Peripheral Blood Mononuclear Cells (PBMC) From Chronic Periodontitis Subjects

This study has been completed.
Sponsor:
ClinicalTrials.gov Identifier:
NCT00990041
First Posted: October 6, 2009
Last Update Posted: October 6, 2009
The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details.
Collaborator:
FOP-UNICAMP
Information provided by:
Escola Bahiana de Medicina e Saude Publica
October 5, 2009
October 6, 2009
October 6, 2009
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No Changes Posted
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Release of Cytokines by Stimulated Peripheral Blood Mononuclear Cells (PBMC) From Chronic Periodontitis Subjects
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Background and Objective: It has been reported that peripheral blood mononuclear cells (PBMC) from periodontal patients have a different profile of cytokine release when compared to the ones of healthy patients. Cigarette smoking is a recognized risk factor for periodontitis and is known to affect the systemic and local immune responses. Thus, the aim of the present study was to obtain preliminary data about the interaction of periodontal condition and smoking in the secretion of some cytokines by PBMC.

Material and Methods: PBMC samples were isolated from 19 donors, divided into generalized chronic periodontitis (P; n=10) and periodontal health (H; n=9) groups. In addition, the smokers and non-smokers of each group were separately evaluated: SP- smokers with severe generalized chronic periodontitis (n=5); SH- periodontally healthy smokers (n=5); NSP- non-smokers with severe generalized chronic periodontitis (n=5) and NSH- periodontally healthy non-smokers (n=4). Cells were incubated for 24-48 hours in 500 µL wells containing RPMI 1640 and stimulated with 1.0 ng/mL of E. coli LPS. Supernatants were used to quantify the amounts of TNF-α, IL-6, IL-8 and IL-10 released using ELISA kits.

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Observational
Observational Model: Case Control
Time Perspective: Cross-Sectional
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Non-Probability Sample
healthy humans
Periodontitis
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  • PBMC
  • periodontitis
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*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Completed
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Inclusion Criteria:

  • periodontitis

Exclusion Criteria:

  • diabetes
Sexes Eligible for Study: All
25 Years to 55 Years   (Adult)
Yes
Contact information is only displayed when the study is recruiting subjects
Brazil
 
 
NCT00990041
9002-10-04.PBMC
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Escola Bahiana de Medicina e Saude Publica
FOP-UNICAMP
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Escola Bahiana de Medicina e Saude Publica
October 2009