Effect of Ischemic Postconditioning on Targeting of Annexin A5 After Forearm Exercise

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT00441714
Recruitment Status : Withdrawn (new insights have let us to expect marginal annexin targeting with a 10 minutes delay between reperfusion and administration of Annexin A5)
First Posted : March 1, 2007
Last Update Posted : November 10, 2015
Information provided by:
Radboud University

February 28, 2007
March 1, 2007
November 10, 2015
October 2007
Not Provided
Annexin A 5 targeting in the non dominant thenar muscle after (ischemic exercise), as a indicator for ischemia reperfusion injury.
Same as current
Complete list of historical versions of study NCT00441714 on Archive Site
Workload during (ischemic) exercise.
Same as current
Not Provided
Not Provided
Effect of Ischemic Postconditioning on Targeting of Annexin A5 After Forearm Exercise
Effect of Ischemic Postconditioning on Targeting of Annexin A5 After Forearm Exercise

To adjust our forearm model for ischemia reperfusion injury, in order to used it for assessment of ischemia reperfusion damage in elderly and patients.

To test the effect of ischemic postconditioning on ischemia reperfusion injury in healthy volunteers, using Annexin binding after repetitive handgripping.

Recently, ischemic postconditioning has been identified as a protective intervention against ischemia-reperfusion injury. In animal studies, the signalling pathway of this (impressive) protective phenomenon is very similar to ischemic preconditioning. It opens a new avenue of post-reperfusion interventions with drugs that have been shown to mimic ischemic preconditioning. Before we can study this phenomenon in our forearm ischemia-reperfusion model, we need additional validating experiments. The purpose of this study proposal is to provide these data.
Not Applicable
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: None (Open Label)
Primary Purpose: Diagnostic
  • Cardiovascular Disease
  • Ischemia-reperfusion Injury
  • Postconditioning
Procedure: repetitive short ischemia and reperfusion of non dominant fore arm after 10 minute ischemic exercise
Not Provided
Rongen GA, Oyen WJ, Ramakers BP, Riksen NP, Boerman OC, Steinmetz N, Smits P. Annexin A5 scintigraphy of forearm as a novel in vivo model of skeletal muscle preconditioning in humans. Circulation. 2005 Jan 18;111(2):173-8. Epub 2004 Dec 27.

*   Includes publications given by the data provider as well as publications identified by Identifier (NCT Number) in Medline.
December 2007
Not Provided

Inclusion Criteria:

  • Age 18-50 years
  • No physical limitation to perform ischemic exercise
  • Informed consent

Exclusion Criteria:

  • Diabetes (fasting glucose > 7,0mmol/l, or random glucose >11,0mmol/l)
  • hyperlipidemia (random total cholesterol > 6.5 mmol/l)
  • Hypertension (supine SBP/DBP > 140/90 mmHg at screening)
  • Any cardiovascular disease
  • Drug abuse
  • Concomitant chronic use of medication
  • Administration of radioactivity in research setting during the last 5 years
  • Participation to any drug-investigation during the previous month as checked with VIP check according to CRCN standard procedures.
Sexes Eligible for Study: Male
18 Years to 50 Years   (Adult)
Contact information is only displayed when the study is recruiting subjects
Not Provided
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Radboud University
Not Provided
Principal Investigator: Gerard Rongen, MD PhD RUMCN
Radboud University
November 2015

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP