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Oral 'Breath Test' to Measure Anabolic Sensitivity in Young and Older Adults at Different Activity Levels (ARBT)

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ClinicalTrials.gov Identifier: NCT05216809
Recruitment Status : Recruiting
First Posted : February 1, 2022
Last Update Posted : June 27, 2022
Sponsor:
Information provided by (Responsible Party):
Daniel Moore, University of Toronto

Brief Summary:
Recent work in the investigators laboratory has examined the ability of a non-invasive 13CO2 breath-test to assess differences in amino acid oxidation rates and net balance in young healthy males following protein feeding and resistance exercise. The investigators aim to test the efficacy of this non-invasive 13CO2 breath-test to assess for differences in anabolic sensitivity between young and older adults following an acute period of habitual and reduced physical activity.

Condition or disease Intervention/treatment Phase
Sedentary Behavior Aging Sarcopenia Amino Acids Dietary Protein Behavioral: Habitual Physical Activity Behavioral: Step-Reduction Not Applicable

Detailed Description:

Aging is associated with a phenomenon known as anabolic resistance, whereby individuals are less responsive to anabolic stimuli (i.e., resistance exercise, dietary protein ingestion). Mild and severe reductions in physical activity are also known to play a key role in the emergence of anabolic resistance. Therefore, the purpose of this study is to investigate the ability of a non-invasive 13CO2 breath-test to assess differences in whole-body leucine oxidation and leucine net balance between younger and older individuals under conditions of habitual activity and following a period of reduced activity/step-reduction. In this manner, the ability of the methodology to distinguish anabolic sensitivity between young and older adult populations will be assessed.

Methods:

Total participant time commitment will span over 4 sessional dates:

Session 1 - Introduction Interested participants will be recruited to engage in a video conference call to undergo an introductory session. Participants will be provided with a comprehensive introduction to the study which will explain the research objectives, conduct, and associated risks. Participants will provide informed consent prior to the collection of any study information (i.e., anthropometrics and habitual activity levels). Eligibility will also be screened for at this time.

Session 2 - Body Composition Assessment Participants will consume a bolus of deuterium oxide (D2O) and provide saliva samples in order to estimate fat-free mass.

The remainder of the study will consist of seven consecutive days, wherein Phase 1 (Habitual Activity) will be subsequently followed by Phase 2 (Step-Reduction).

Phase 1 - Habitual Activity (Days 1-3) Participants will be asked to engage in their habitual activity pattern (> 7000 steps/day) while wearing an accelerometer and pedometer. Participants will be further instructed to maintain their normal dietary patterns and to track their diets over the course of these three days using diet logs.

Session 3 - Habitual Activity Metabolic Trial (Day 4) On the morning of the fourth day, following three days of habitual activity, participants will be subjected to the non-invasive 13CO2 breath-test to assess for whole-body leucine oxidation and net balance. This date will also serve as a lead-in to the subsequent Phase 2.

Phase 2 - Step-Reduction (Days 4-6) Participants will be asked to maintain their normal dietary patterns and track their dietary intake, but will need to adhere to a reduced daily step count (<2000 steps/day). Accelerometers and pedometers will be worn again during this period.

Session 4 - Step-Reduction Metabolic Trial (Day 7) On the morning of the 7th day, following three days of reduced daily step-count, participants will be subjected again to the non-invasive 13CO2 breath-test to assess for whole-body leucine oxidation and net balance. Ultimately, this will allow us to determine whether 3-days of reduced step-count will impart any changes on anabolic sensitivity.

For each metabolic trial, two urine samples (baseline and pooled enriched) will be collected, in addition to breath samples collected over a 6-hour post-prandial period at 20-30 minute intervals. These biological samples will be used to measure tracer excretion and oxidation, allowing us to determine rates of protein metabolism.

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Study Type : Interventional  (Clinical Trial)
Estimated Enrollment : 32 participants
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Intervention Model Description:

Parallel Group Cohort Study

Twenty healthy young (18-35 years of age; 10 females and 10 males) and twelve healthy older adults (60-80 years of age; 6 females and 6 males), for a total of 32 individuals, will undergo 3 days (Days 1-3) of Habitual Activity in a free-living setting (Phase 1) followed by a metabolic trial (Day 4), where individuals will be subjected to a non-invasive 13CO2 breath-test. This will also serve as a lead-in period for the subsequent 3 days of step-reduction (<2,000 steps/day; Days 4-6). Following the 3-days of reduced daily step count (Day 7), participants will undergo another metabolic trial day involving the non-invasive 13CO2 breath-test in order for us to determine changes in anabolic sensitivity.

Masking: None (Open Label)
Primary Purpose: Other
Official Title: The Efficacy of a Non-Invasive 13CO2 'Breath Test' to Detect Anabolic Resistance Following Step Reduction in Younger and Older Adults
Actual Study Start Date : February 1, 2022
Estimated Primary Completion Date : September 2022
Estimated Study Completion Date : November 2022

Arm Intervention/treatment
Active Comparator: Young Adults
Equal sex distribution (10M/10F); ages 18-35 years old.
Behavioral: Habitual Physical Activity
Participants will maintain habitual levels of physical activity (inclusive of structured physical activity). Participants will be required to maintain >7,000 steps/day.
Other Name: Free-Living Activity

Behavioral: Step-Reduction
Participants will be required to reduce their daily step-counts to <2,000 steps/day. Further, they will be required to refrain from structured physical activity.
Other Name: Reduced Physical Activity

Active Comparator: Older Adults
Equal sex distribution (6M/6F); ages 60-80 years old.
Behavioral: Habitual Physical Activity
Participants will maintain habitual levels of physical activity (inclusive of structured physical activity). Participants will be required to maintain >7,000 steps/day.
Other Name: Free-Living Activity

Behavioral: Step-Reduction
Participants will be required to reduce their daily step-counts to <2,000 steps/day. Further, they will be required to refrain from structured physical activity.
Other Name: Reduced Physical Activity




Primary Outcome Measures :
  1. Exogenous Leucine Oxidation (umol/kg) [ Time Frame: 6 hours ]
    Exogenous Leucine Oxidation determined from breath 13CCO2 enrichment. Breath samples will be collected every 20-30min after test drink ingestion to determine breath 13CO2 enrichment. Total leucine oxidation will be determined from the area under the 13CO2 enrichment by time curve.

  2. Net Leucine Retention (umol/kg) [ Time Frame: 6 hours ]
    Whole-Body Net Leucine Retention determined from the difference between exogenous leucine oxidation and leucine ingestion the 6 hour measurement period.



Information from the National Library of Medicine

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Ages Eligible for Study:   18 Years to 80 Years   (Adult, Older Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes
Criteria

Inclusion Criteria:

  • Healthy young (age: 18-35 years) or older (age: 60-80 years) adults
  • Average daily step-count >7,000/day
  • BMI between normal to overweight (18.5-29.9 kg/m2)

Exclusion Criteria:

  • Regular use of nonsteroidal anti-inflammatory drugs (with the exception of daily low-dose aspirin)
  • Use of anticoagulants
  • Use of a walker, cane, or assistive walking device
  • Current or recently remised cancer
  • Infectious or gastrointestinal disease
  • Inability to comply with study protocol (e.g., >2,000 steps/day during Step-Reduction Phase)
  • Regular tobacco use
  • Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.)
  • Diagnosed chronic illness (e.g. type 2 diabetes, heart disease, thyroid disease)
  • Pregnant
  • Hormonal Replacement Therapy

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT05216809


Contacts
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Contact: Daniel R Moore, PhD 4169464088 dr.moore@utoronto.ca
Contact: Matthew Lees, PhD matthew.lees@utoronto.ca

Locations
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Canada, Ontario
Goldring Centre for High Performance Sport at the University of Toronto Recruiting
Toronto, Ontario, Canada, M5S2C9
Contact: Daniel R Moore, PhD    416-946-4088    dr.moore@utoronto.ca   
Contact: Hugo Fung, MSc       hugojernwai.fung@mail.utoronto.ca   
Sponsors and Collaborators
University of Toronto
Investigators
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Principal Investigator: Daniel Moore, PhD University of Toronto
Study Director: Hugo JW Fung, PhD (c) University of Toronto
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Responsible Party: Daniel Moore, Principal Investigator, University of Toronto
ClinicalTrials.gov Identifier: NCT05216809    
Other Study ID Numbers: ARBT
First Posted: February 1, 2022    Key Record Dates
Last Update Posted: June 27, 2022
Last Verified: June 2022
Individual Participant Data (IPD) Sharing Statement:
Plan to Share IPD: No

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Studies a U.S. FDA-regulated Drug Product: No
Studies a U.S. FDA-regulated Device Product: No
Keywords provided by Daniel Moore, University of Toronto:
Skeletal Muscle
Step-Reduction
Aging
Anabolic Resistance
Physical Activity
Stable Isotopes
Additional relevant MeSH terms:
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Sarcopenia
Muscular Atrophy
Neuromuscular Manifestations
Neurologic Manifestations
Nervous System Diseases
Atrophy
Pathological Conditions, Anatomical