Biocollection in MyeloDysplastic Syndrome (P-MDS) (P-MDS)
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|ClinicalTrials.gov Identifier: NCT04869683|
Recruitment Status : Recruiting
First Posted : May 3, 2021
Last Update Posted : April 18, 2023
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|Condition or disease||Intervention/treatment||Phase|
|Myelodysplastic Syndromes Myelodysplastic Anemia Myelodysplastic Syndrome With Isolated Del(5Q) Myelodysplastic Syndrome With Ring Sideroblasts Acute Myeloid Leukemia With Multilineage Dysplasia Chromosome Abnormality||Other: description of MDS pzatient cohort||Not Applicable|
The objective of the biocollection is to respond to 3 scientific projects in MDS :
Project 1: splicing anomalies in MDS with SF3B1 mutations : About 95% of the coding genes in humans are subjected to alternative splicing, a complex, highly regulated mechanism that diversifies the proteome by defining multiple proteins from a single gene. Deregulation of splicing is observed in many cancers and hemopathies (review Yoshida et al., 2014), especially in myelodysplastic syndromes More than half of MDS patients present an acquired mutation in a gene involved in the splicing of pre -RNA messengers. The SF3B1 gene (splice factor 3B subunit 1), which encodes a protein involved in the recognition of 3 'splice sites is the most frequently mutated gene in SMDs, at a frequency of 20-28% MDS, and up to 85% of myelodysplastic syndromes with crowned sideroblasts (Yoshida et al., 2011, Papaemmanuil et al., 2011). The functional consequences of the splicing abnormalities thus generated on the pathophysiology of MDS are far from clear. The transcriptome analyzes (by RNA-seq) carried out recently in various acquired pathologies that the most frequent variants of the SF3B1 gene lead to the formation of aberrant transcripts by the use of a 3 ' cryptic splicing site. The investigators seek to study the functional implications of SF3B1 mutations found in MDS patients in particular on the formation of sideroblasts in the crown. The investigators want to identify, by RNAseq, the aberrant junctions specifically expressed in the cells of MDS patients with mutated SF3B1, and which would affect transcripts of genes involved in iron metabolism or its regulation. For this, the investigators will use the cells obtained from the marrow of SMD SF3B1WT patients versus SF3B1K700E and collected in the Chromosomal Genetics laboratory, site of the CRB of the CHRU of Brest. The investigators will then analyze the functional repercussions associated with the presence of these aberrant junctions on the cells in culture of these same patients (detection of certain proteins, enzymatic analyzes, etc.). The collection of biological and clinical data from these patients of interest is essential for the interpretation of the results. This project is part of a more global approach to the study of the various splicing anomalies in this pathology.
Project 2: splicing abnormalities in MDS with chromosomal abnormalities as 5q deletion : Chromosome 5 deletions are the most frequent structural abnormalities in MDS and constitute a good prognostic entity if isolated or associated with an anomaly and poor prognosis if associated with more than 3 chromosomal abnormalities. Two genes located on chromosomes 5 encode proteins of a complex involved in the splicing of pre-messenger RNAs: the RBM22 gene (RNA Binding Motif Protein 22) and the SLU7 gene (SLU7 Homolog Splicing Factor).
The investigators want to identify subgroups of patients with loss of these 2 genes or loss of RBM22 and conservation of SLU7. Does the loss of one or both genes play a role in the pathophysiogenesis of MDS with chromosome deletions and is it associated with worsening of the disease? Understanding these mechanisms could also have an impact on the therapeutic management of this pathology.
The study of these chromosomal abnormalities associated with splicing abnormalities in MDS for the chromosomal genetics laboratory both from a diagnostic and research perspective. Several works carried out have given rise to numerous scientific publications with an international reading committee for several years.
Project 3: Evolution of MDS in acute myeloid leukemia (AML) More than one third of patients with MDS progress to AML. The investigators want to focus on this group of patients and understand the clonal architecture of their malignant cells to detect new predictive markers of indolent or rapid disease progression.
|Study Type :||Interventional (Clinical Trial)|
|Estimated Enrollment :||150 participants|
|Intervention Model:||Single Group Assignment|
|Intervention Model Description:||Health services research and follow up DMS|
|Masking:||None (Open Label)|
|Primary Purpose:||Health Services Research|
|Official Title:||Biocollection in Patients With Myelodysplastic Syndrome (P-MDS)|
|Actual Study Start Date :||October 19, 2022|
|Estimated Primary Completion Date :||October 19, 2032|
|Estimated Study Completion Date :||October 19, 2032|
MDS follow up
it is a description MDS patients study
Other: description of MDS pzatient cohort
description of MDS patient cohort
- Cohort MDS: epidemiologic MSD study [ Time Frame: five years ]MDS study in collecting data
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|Ages Eligible for Study:||18 Years and older (Adult, Older Adult)|
|Sexes Eligible for Study:||All|
|Accepts Healthy Volunteers:||Yes|
- patient with or suspected of myelodysplastic syndrome (WHO definition) at diagnosis and/or during follow-up, which is managed at the level of the Cancer-Hematology Institute of the Brest CHRU
- Presence of biological material collected within the CRB
- Patient's consent obtained
Exclusion Criteria :
- Minor and pregnant woman
- Lack of biological material collected within the CRB
- Refusal to participate: lack of consent - Unable to consent
- Patient under judicial protection: guardianship, curatorship ...
To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.
Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT04869683
|Contact: Nathalie Douet-Guilbert, MD, PhDemail@example.com|
|Contact: Marie-Bérengère Troadec, PhDfirstname.lastname@example.org|
|Brest, France, 29609|
|Contact: Nathalie DOUET-GUILBERT|
|Principal Investigator:||Nathalie Douet-Guilbert, MD,PhD||CHRU Brest|
|Responsible Party:||University Hospital, Brest|
|Other Study ID Numbers:||
|First Posted:||May 3, 2021 Key Record Dates|
|Last Update Posted:||April 18, 2023|
|Last Verified:||April 2023|
|Individual Participant Data (IPD) Sharing Statement:|
|Plan to Share IPD:||Yes|
|Plan Description:||All collected data that underlie results in a publication|
|Time Frame:||Data will be available after the publication of result and ending fifteen years following the last visit of the last patient|
|Access Criteria:||Data access requests will be reviewed by the internal committee of Brest UH. Requestors will be required to sign and complete a data access agreement|
|Studies a U.S. FDA-regulated Drug Product:||No|
|Studies a U.S. FDA-regulated Device Product:||No|
Bone Marrow Diseases
Genetic Diseases, Inborn