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Acute Whey Protein And Casein Supplementation: Effect On Protein Metabolism After Resistance Exercise

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ClinicalTrials.gov Identifier: NCT04648384
Recruitment Status : Completed
First Posted : December 1, 2020
Last Update Posted : December 2, 2020
Sponsor:
Collaborators:
Fundação de Amparo à Pesquisa do Estado de São Paulo
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnología (COLCIENCIAS)
Information provided by (Responsible Party):
Ellen Cristini de Freitas, University of Sao Paulo

Brief Summary:
The proteins of human breast milk are the most important endocrine signaling system that promotes neonatal growth by increasing the release of insulin, insulin-like growth factor-1 (IGF-1), and the leucine-mediated mammalian target of rapamycin complex 1- (mTORC1) signaling of pancreatic β-cells. Remarkably, the branched-chain amino acids (BCAAs) leucine, isoleucine, and valine are involved in the growth-promoting effects of milk, protein biosynthesis, and metabolism because they physiologically stimulate insulin secretion The present study sought to investigate the effects of the combination of whey protein and casein in the ratio of 80:20 ("whey protein: casein" or "casein: whey protein") as breast milk proportion on the peak and the period of the permanence of branched-chain amino acids in the blood circulation, final metabolites of protein metabolism and delayed onset muscle soreness (DOMS) after a single bout of fasting or a resistance exercise session.

Condition or disease Intervention/treatment Phase
Sports Nutrition Protein Supplementation Dietary Supplement: Blend Protein Supplementation Dietary Supplement: Placebo Supplementation Other: Resistance Exercise Not Applicable

Detailed Description:
The present study investigated the effects of the association of WP and casein in the ratio of 80:20, a similar ratio of human breast milk, on blood branched-chain amino acids (BCAAs) profile by HPLC technique, markers of protein metabolism (creatinine, urea, and urine nitrogen) and delayed onset muscle soreness (DOMS) after a single bout of resistance exercise. A double-blind, crossover and acute study was conducted with ten men (age 29 ± 8 y; BMI: 25.4 ± 2.9 Kg/m2; 77 ± 12 kg; 1.74 ± 0.09 m) that were randomly assigned to five supplementation treatments: WP - whey protein; CAS - casein; WP/CAS - 80% WP/20% CAS; CAS/WP - 80% CAS/ 20% WP; PLA - placebo. Participants were submitted to the following evaluations: performance sessions, blood collection for each session for the BCAAs profile determination, two food records, 3-days assessment of DOMS (24h, 48h, 72h) after each treatment.

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Study Type : Interventional  (Clinical Trial)
Actual Enrollment : 10 participants
Allocation: Randomized
Intervention Model: Crossover Assignment
Masking: Double (Participant, Investigator)
Primary Purpose: Basic Science
Official Title: Association of Whey Protein and Casein in the Same Proportions of Human Breast Milk Promoted Similar Branched-chain Amino Acids Profile and Reduced Delayed Muscle Soreness in Physically Active Man
Actual Study Start Date : March 20, 2016
Actual Primary Completion Date : July 20, 2019
Actual Study Completion Date : October 20, 2019

Resource links provided by the National Library of Medicine

Drug Information available for: Casein

Arm Intervention/treatment
Experimental: Protein Supplementation and Resistance exercise
Protein supplementation composed of 20 gram of whey protein or casein in different proportions (80:20) and fasting state 10 sets of 10 reps at 85% of 1 Maximum repetition
Dietary Supplement: Blend Protein Supplementation
Protein supplementation using 20 grams of whey protein or casein mix. Acute Resistance training, 10 sets of 10 reps at 85% of 1Maximum repetition
Other Name: Resistance Training

Other: Resistance Exercise
10 sets of 10 repetitions at 85% of 1 Maximum repetition in leg press exercise

Placebo Comparator: Placebo Supplementation
Placebo supplementation composed of 20 gram of Maltodextrin and fasting state 10 sets of 10 reps at 85% of 1 Maximum repetition
Dietary Supplement: Placebo Supplementation
Placebo supplementation using 20 grams of Maltodextrin. Acute Resistance training, 10 sets of 10 reps at 85% of 1Maximum repetition
Other Name: Resistance Training

Other: Resistance Exercise
10 sets of 10 repetitions at 85% of 1 Maximum repetition in leg press exercise

Experimental: Resistance Training
Resistance Exercise will be performed before supplementation and is composed by 10 sets of 10 repetitions at 85% of 1 Maximum repetition in leg press exercise.
Other: Resistance Exercise
10 sets of 10 repetitions at 85% of 1 Maximum repetition in leg press exercise




Primary Outcome Measures :
  1. Change from baseline in plasma amino acids concentration [ Time Frame: 5 weeks ]
    Blood sample collected for analysis of BCAA concentration after blend protein or placebo supplementation expressed in (µmol/L)

  2. Change from baseline in 1RM test [ Time Frame: 5 weeks ]
    Change of training load using RPE, time and load expressed in (a.u)

  3. Change in protein metabolism [ Time Frame: 5 weeks ]
    Change in nitrogen excretion, and nitrogen balance after protein supplementation expressed in (g/24 hours)

  4. Changes from baseline in macronutrient intake [ Time Frame: 5 weeks ]
    Change of macronutrient intake were calculated pre and post treatment expressed in (g/24 hours)

  5. Change in delayed onset muscle soreness [ Time Frame: 5 weeks ]
    Change from baseline the delayed onset muscle soreness pre, post, 24 hours, 48 hours and 72 hours after treatment expressed in (a.u)



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Ages Eligible for Study:   20 Years to 40 Years   (Adult)
Sexes Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes
Criteria

Inclusion Criteria:

  • Healthy
  • practice resistance training at least 3x a week

Exclusion Criteria:

  • Smokers
  • Muscle injures
  • cardiovascular diseases
  • Diabetes mellitus
  • Lactose intolerance

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT04648384


Locations
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Brazil
University of São Paulo, School of Physical Education and Sports of Ribeirão Preto.
Ribeirão Preto, São Paulo, Brazil, 14049-900
Sponsors and Collaborators
University of Sao Paulo
Fundação de Amparo à Pesquisa do Estado de São Paulo
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnología (COLCIENCIAS)
Publications automatically indexed to this study by ClinicalTrials.gov Identifier (NCT Number):
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Responsible Party: Ellen Cristini de Freitas, Principal Investigator, University of Sao Paulo
ClinicalTrials.gov Identifier: NCT04648384    
Other Study ID Numbers: Protein Supplementation
First Posted: December 1, 2020    Key Record Dates
Last Update Posted: December 2, 2020
Last Verified: November 2020

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Studies a U.S. FDA-regulated Drug Product: No
Studies a U.S. FDA-regulated Device Product: No