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Neoantigen-based Personalized DNA Vaccine in Patients With Newly Diagnosed, Unmethylated Glioblastoma

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ClinicalTrials.gov Identifier: NCT04015700
Recruitment Status : Not yet recruiting
First Posted : July 11, 2019
Last Update Posted : September 25, 2019
Sponsor:
Information provided by (Responsible Party):
Washington University School of Medicine

Brief Summary:
This is a single institution, open-label, single arm, study assessing the safety, feasibility, and immunogenicity of a personalized neoantigen-based vaccine in subjects with newly diagnosed, unmethylated glioblastoma.

Condition or disease Intervention/treatment Phase
Glioblastoma Biological: Personalized neoantigen DNA vaccine supplied by Geneos Therapeutics Device: CELLECTRA®2000 EP Device supplied by Geneos Therapeutics Drug: Plasmid encoded IL-12 Phase 1

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Study Type : Interventional  (Clinical Trial)
Estimated Enrollment : 6 participants
Intervention Model: Single Group Assignment
Masking: None (Open Label)
Primary Purpose: Treatment
Official Title: A Pilot Study to Assess the Safety, Feasibility, and Immunogenicity of a Neoantigen-based Personalized in Patients With Newly Diagnosed, Unmethylated Glioblastoma
Estimated Study Start Date : December 31, 2019
Estimated Primary Completion Date : March 31, 2023
Estimated Study Completion Date : December 31, 2023

Resource links provided by the National Library of Medicine

Drug Information available for: Coal Tar

Arm Intervention/treatment
Experimental: Vaccine (GNOS-PV01 + INO-9012)
  • Standard radiation therapy will be administered per standard of care and is outside the scope of this study.
  • GNOS-PV01 + INO-9012 will be given on Days 1, 22, and 43 of Cycle 1 and then on Day 1 of each subsequent cycle beginning with Cycle 2 for a total of 6 cycles or until intolerance or progression
Biological: Personalized neoantigen DNA vaccine supplied by Geneos Therapeutics
-The neoantigen DNA vaccines are also known as DNA plasmid vector expressing tumor-specific antigens.
Other Names:
  • GNOS-PV01
  • Vaccine

Device: CELLECTRA®2000 EP Device supplied by Geneos Therapeutics
CELLECTRA® 2000 Device is a system indicated for use to enhance the uptake and expression of plasmid-based biologics in order to enhance vaccine efficacy.

Drug: Plasmid encoded IL-12
The INO-9012 vials will be supplied by Geneos Therapeutics
Other Name: INO-9012




Primary Outcome Measures :
  1. Safety and tolerability of a personalized neoantigen DNA vaccine as measured by dose-limiting toxicities (DLTs) [ Time Frame: Up to 30 days ]
    A DLT will be defined as any grade 3 toxicity or greater according to CTCAE v5 considered at least possibly related to study treatment. The DLT observation period begins with Cycle 1 Day 1 (date of first vaccine administration) and continues for 30 days

  2. Feasibility of generating a personalized neoantigen DNA vaccine for patients with newly diagnosed, unmethylated GBM as measured by the ability to identify candidate tumor-specific neoantigens [ Time Frame: 4 weeks post-completion of radiotherapy (day 1 of treatment regimen) ]
  3. Feasibility of generating a personalized neoantigen DNA vaccine for patients with newly diagnosed, unmethylated GBM as measured by the ability to manufacture a neoantigen-based DNA vaccine [ Time Frame: 4 weeks post-completion of radiotherapy (day 1 of treatment regimen) ]
  4. Feasibility of generating a personalized neoantigen DNA vaccine for patients with newly diagnosed, unmethylated GBM as measured by the ability to administer the vaccine to a patient [ Time Frame: 4 weeks post-completion of radiotherapy (day 1 of treatment regimen) ]

Secondary Outcome Measures :
  1. Immunogenicity of a personalized neoantigen DNA vaccine as measured by the ability to generate a measurable neoantigen-specific CD8 T cell response in vaccinated patients [ Time Frame: Week 10 post-vaccination ]
  2. Immunogenicity of a personalized neoantigen DNA vaccine as measured by the number of individual neoantigens per number of neoantigens vaccinated against, with which a measurable CD8 T cell-specific response is identified [ Time Frame: Week 10 post-vaccination ]
  3. Number of high quality candidates neoantigens present in patients with newly diagnosed GBM [ Time Frame: Week 24 post-vaccination ]
    -High quality neoantigens will be defined as those that meet criteria for inclusion in a vaccine

  4. Progression-free survival (PFS) rate [ Time Frame: 6 months ]
    • PFS=duration of time from start of treatment to time of progression or death, whichever occurs first.
    • Progression: any of the following

      • ≥ 25% increase in sum products of perpendicular diameters of enhancing lesions compared with the smallest tumor measurement obtained either at baseline or best response, on stable or increasing doses of corticosteroids*. The absolute increase in any dimension must be at least 5mm when calculating the products.
      • Significant increase in T2/FLAIR nonenhancing lesion on stable/increasing doses of corticosteroids compared with baseline scan or best response after initiation of therapy* not caused by comorbid events
      • New measureable lesion.
      • Clear clinical deterioration not attributable to other causes apart from the tumor (e.g. seizures, medication adverse effects, complications of therapy, cerebrovascular events, infection, and so on) or changes in corticosteroid dose.

  5. Overall survival rate [ Time Frame: 12 months ]
  6. Immunogenicity of a personalized neoantigen DNA vaccine as measured by T-cell phenotype, myeloid derived suppressor cell frequency by flow cytometry [ Time Frame: Week 24 post-vaccination ]
  7. Immunogenicity of a personalized neoantigen DNA vaccine as measured by diversity of clonality from T cell receptor sequencing [ Time Frame: Week 24 post-vaccination ]
  8. Immunogenicity of a personalized neoantigen DNA vaccine as measured by putative antigen specificity from T cell receptor sequencing [ Time Frame: Week 24 post-vaccination ]


Information from the National Library of Medicine

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Ages Eligible for Study:   18 Years and older   (Adult, Older Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   No
Criteria

Inclusion Criteria:

  • Newly diagnosed histologically confirmed MGMT unmethylated glioblastoma multiforme (WHO grade IV). Patients with secondary glioblastoma, in particular those who are IDH1 or IDH2 mutant, will not be excluded. High grade gliomas with molecular features of glioblastoma will be included. MGMT methylation status must be confirmed by standard a PCR-based assay.
  • Patients who had prior craniotomy with biopsy, subtotal resection, total gross resection, or re-resection will be permitted.
  • Consent to genome sequencing and dbGaP-based data sharing and has provided or will provide germline (PBMC) and tumor DNA/RNA samples of adequate quality for sequencing. (Acquisition of specimens for sequencing and the sequencing itself may be done as part of routine care or another research project.)
  • At least 18 years of age.
  • Karnofsky performance status ≥ 60%
  • Normal bone marrow and organ function as defined below:

    • Absolute neutrophil count ≥ 1,500/mcL
    • Platelets ≥ 100,000/mcL
    • Total bilirubin ≤ 1.5 x IULN
    • AST(SGOT)/ALT(SGPT) ≤ 3.0 x IULN
    • Creatinine ≤ IULN OR creatinine clearance ≥ 60 mL/min/1.73 m2 for patients with creatinine levels above institutional normal
  • Systemic corticosteroid therapy is permitted provided dosing is no greater than 4 mg per day (dexamethasone or equivalent) on the day of vaccine administration.
  • Bevacizumab will be allowed if given for symptomatic control of vasogenic edema and to avoid high dose of corticosteroids.
  • Women of childbearing potential and men must agree to use adequate contraception (hormonal or barrier method of birth control, abstinence) prior to study entry and for the duration of study participation, including at least 5 months (for women of childbearing potential) and at least 7 months (for men) after last dose of study drug. Should a woman become pregnant or suspect she is pregnant while participating in this study, she must inform her treating physician immediately.
  • Ability to understand and willingness to sign an IRB approved written informed consent document (or that of legally authorized representative, if applicable).

Exclusion Criteria:

  • As this study aims to assess the immunogenicity of personalized neoantigen DNA vaccine plus plasmid encoded IL-12 as an adjuvant, no prior immunotherapy will be permitted.
  • Inadequate tissue acquisition to allow for neoantigen screening.
  • No candidate neoantigen identified during screening.
  • A history of other malignancy ≤ 3 years previous with the exception of non-melanoma skin cancer, any in situ cancer that has been successfully resected and cured, treated superficial bladder cancer, or any early-stage solid tumor that was successfully resected without need for adjuvant radiation or chemotherapy.
  • Receiving any other investigational agents within 4 weeks of beginning study treatment.
  • Known allergy, or history of serious adverse reaction to, vaccines such as anaphylaxis, hives, or respiratory difficulty.
  • A history of allergic reactions attributed to compounds of similar chemical or biologic composition to any agents used in the study.
  • Uncontrolled intercurrent illness including, but not limited to, ongoing or active infection, symptomatic congestive heart failure, unstable angina pectoris, cardiac arrhythmia, or psychiatric illness/social situations that would limit compliance with study requirements.
  • History of immunodeficiency disorder or autoimmune condition requiring active immunosuppressive therapy. This includes inflammatory bowel disease, ulcerative colitis, Crohn's disease, systemic vasculitis, scleroderma, psoriasis, multiple sclerosis, hemolytic anemia, immune-mediated thrombocytopenia, rheumatoid arthritis, systemic lupus erythematosus, Sjögren's syndrome, sarcoidosis, or other rheumatologic disease or any other medical condition or use of medication which might make it difficult for the patient to complete the full course of treatments or to generate an immune response to vaccines.
  • Presence of clinically significant increased intracranial pressure (e.g. impending herniation) or hemorrhage, uncontrolled seizures, or requirement for immediate palliative treatment.
  • Pregnant and/or breastfeeding. Women of childbearing potential must have a negative pregnancy test within 7 days of first dose of vaccine.
  • Presence of acute or chronic bleeding or clotting disorder that would contraindicate IM injections
  • Presence of a cardioverter-defibrillator or pacemaker (to prevent a life-threatening arrhythmia) that is located ipsilateral to the intended deltoid injection site (unless deemed acceptable by a Cardiologist).
  • Presence of any metal implants or implantable medical device within the electroporation area.

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT04015700


Contacts
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Contact: Tanner M Johanns, M.D., Ph.D. 314-273-2723 tjohanns@wustl.edu

Locations
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United States, Missouri
Washington University School of Medicine Not yet recruiting
Saint Louis, Missouri, United States, 63110
Contact: Tanner M Johanns, M.D., Ph.D.    314-273-2723    tjohanns@wustl.edu   
Principal Investigator: Tanner M Johanns, M.D., Ph.D.         
Sub-Investigator: William E Gillanders, M.D.         
Sub-Investigator: Gavin Dunn, M.D., Ph.D.         
Sub-Investigator: Jian Campian, M.D., Ph.D.         
Sub-Investigator: George Ansstas, M.D.         
Sub-Investigator: Milan G Chheda, M.D.         
Sub-Investigator: Michael Chicoine, M.D.         
Sub-Investigator: Ralph Dacey, M.D.         
Sub-Investigator: Albert Kim, M.D., Ph.D.         
Sub-Investigator: Keith Rich, M.D.         
Sub-Investigator: Feng Gao, Ph.D.         
Sub-Investigator: Joshua Dowling, M.D.         
Sub-Investigator: Eric Leuthardt, M.D.         
Sub-Investigator: Gregory J Zipfel, M.D.         
Sub-Investigator: Joshua Osbun, M.D.         
Sub-Investigator: Jiayi Huang, M.D.         
Sponsors and Collaborators
Washington University School of Medicine
Investigators
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Principal Investigator: Tanner M Johanns, M.D., Ph.D. Washington University School of Medicine

Additional Information:
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Responsible Party: Washington University School of Medicine
ClinicalTrials.gov Identifier: NCT04015700     History of Changes
Other Study ID Numbers: 18-x324
First Posted: July 11, 2019    Key Record Dates
Last Update Posted: September 25, 2019
Last Verified: September 2019
Individual Participant Data (IPD) Sharing Statement:
Plan to Share IPD: No

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Studies a U.S. FDA-regulated Drug Product: Yes
Studies a U.S. FDA-regulated Device Product: Yes
Device Product Not Approved or Cleared by U.S. FDA: No
Pediatric Postmarket Surveillance of a Device Product: No
Product Manufactured in and Exported from the U.S.: No
Additional relevant MeSH terms:
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Glioblastoma
Astrocytoma
Glioma
Neoplasms, Neuroepithelial
Neuroectodermal Tumors
Neoplasms, Germ Cell and Embryonal
Neoplasms by Histologic Type
Neoplasms
Neoplasms, Glandular and Epithelial
Neoplasms, Nerve Tissue
Coal Tar
Vaccines
Immunologic Factors
Physiological Effects of Drugs
Keratolytic Agents
Dermatologic Agents