In-vitro Effect of Mangosteen Pericarp Extract on Cell Lines (invitro)
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|ClinicalTrials.gov Identifier: NCT03728192|
Recruitment Status : Completed
First Posted : November 2, 2018
Last Update Posted : November 2, 2018
|Condition or disease||Intervention/treatment||Phase|
|Apoptosis||Other: mangosteen extract||Not Applicable|
|Study Type :||Interventional (Clinical Trial)|
|Actual Enrollment :||2 participants|
|Intervention Model:||Parallel Assignment|
|Intervention Model Description:||The study comprised of two groups A (Hela) and group B ( H357) . Each group was further subdivided into 3 subgroups- A1 -Untreated cells, A2- mangosteen treated cells and A3- cells treated with standard drug. Group B was divided into B1- Untreated cells, B2- mangosteen treated cells and B3 -cells treated with the standard drug healthy periodontium. Test group: (Chronic periodontitis patients) comprised of 25 participants with signs of clinical inflammation and bone los|
|Masking:||None (Open Label)|
|Official Title:||Inhibition and Cytotoxic Effects of Mangosteen on Cell Lines|
|Actual Study Start Date :||March 7, 2016|
|Actual Primary Completion Date :||May 11, 2016|
|Actual Study Completion Date :||July 13, 2018|
Experimental: Mangosteen treated group
HeLa and H357 cell lines were procured and were further subdivided into 2 subdivisions and were assigned interventions:
Mangosteen group- cells treated with mangosteen extract and Camptothecin group - cells treated with standard anticancer drug camptothecin(25 micro mole)
Other: mangosteen extract
For Mangosteen group :DNA fragmentation assay is used to identify apoptosis.1×106 cells were harvested and treated with mangosteen pericarp extract for 48 h For Camptothecin group : Cells were also treated with standard anticancer drug Camptothecin to act as a positive control.
Other Name: camptothecin
No Intervention: Untreated group
H357 and HeLa cell line without any drug intervention.
- apoptotic potential of ethanolic extract of mangosteen pericarp on oral and cervical cancer cell lines via apoptotic assay [ Time Frame: 48 hrs at base line ]Apoptosis assay was performed using Annexin V/FITC Kit (BD Biosciences, Catalog no. 556547), and the fluorescence intensities of FITC-conjugated annexin-V and Propidium iodide (PI) in cells were analyzed using flow cytometry. HeLa and H357 cells (1×106 cells/well) were seeded in a 6-well plate. The cells were allowed to adhere for 12 hrs, cultured in medium containing different concentrations of mangosteen extract for 48hr. The cells were then collected and washed twice with Phosphate buffer Saline, gently resuspended in 100μL annexinV-FITC binding buffer (1x) and incubated with 5μL annexinV-FITC in the dark for 10 min at 25°C. This was followed by centrifugation of cells at 2000 rpm for 5 min, and gently resuspended in 500μL annexinV-FITC binding buffer (1x) and 5μL PI was added in an ice bath, followed by immediate analysis by flow cytometry Cell Quest software (BD Biosciences).
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Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT03728192
|Principal Investigator:||Jaideep Mahendra, MDS,PhD||Meenakshi academy of higher education and research|