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Oxidative Stress in Testicular Tissue Before and After Cryopreservation

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details.
 
ClinicalTrials.gov Identifier: NCT03621995
Recruitment Status : Completed
First Posted : August 9, 2018
Last Update Posted : July 16, 2019
Sponsor:
Information provided by (Responsible Party):
Aya Hasan, Assiut University

Brief Summary:
The absence of sperms in ejaculated semen, is the most severe form of male-factor infertility and is present in approximately 5 % of all investigated infertile couples.

Condition or disease Intervention/treatment
Oxidative Stress Other: Malondialdehyde and Catalase level measurement

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Study Type : Observational
Actual Enrollment : 58 participants
Observational Model: Case-Only
Time Perspective: Retrospective
Official Title: Oxidative Stress in Testicular Tissue Before and After Cryopreservation
Actual Study Start Date : September 10, 2018
Actual Primary Completion Date : October 10, 2018
Actual Study Completion Date : November 30, 2018

Group/Cohort Intervention/treatment
Obstructive azoospermia
Malondialdehyde and Catalase level measurement before and after cryopreservation
Other: Malondialdehyde and Catalase level measurement
Malondialdehyde and Catalase level measurement by colorimetric method

Non obstructive azoospermia
Malondialdehyde and Catalase level measurement before and after cryopreservation
Other: Malondialdehyde and Catalase level measurement
Malondialdehyde and Catalase level measurement by colorimetric method

Negative group
Malondialdehyde and Catalase level measurement without cryopreservation
Other: Malondialdehyde and Catalase level measurement
Malondialdehyde and Catalase level measurement by colorimetric method




Primary Outcome Measures :
  1. Measurement of the level of oxidative stress that is caused by cryopreservation of testicular tissue. [ Time Frame: 3 years ]

    Evaluation of oxidative stress caused by cryopreservation of testicular tissue by measurement of :

    1. Catalase activity as one indicator of antioxidants by colorimetric method.
    2. Malondialdehyde level as one indicator of lipid peroxidation by colorimetric method.



Information from the National Library of Medicine

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Ages Eligible for Study:   14 Years to 80 Years   (Child, Adult, Older Adult)
Sexes Eligible for Study:   Male
Sampling Method:   Probability Sample
Study Population

45 patients complaining of azoospermia who attended to the Andrology Outpatient Clinic, Dermatology ,Venerology and Andrology department, Assuit University Hospital, Assuit ,Egypt .Patients were divided into 3 groups:

  • Group A : 15 patients with obustructive azoospermia and positive testicular samples for sperms .
  • Group B : 15 patients with non obustructive azoospermia and positive testicular samples for sperms.
  • Group C : 15 patients with azoospermia and negative testicular samples for spems.
Criteria

Inclusion Criteria:

- Men with azoospermia at reproductive age .

Exclusion Criteria:

  • Pyospermia.
  • Previous trial of cryopreservation.
  • Systemic diseases.
  • Cryptorchidism history.
  • Smoking

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT03621995


Locations
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Egypt
Assuit University Hospital
Assiut, Egypt, 71511
Sponsors and Collaborators
Assiut University
Investigators
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Principal Investigator: Aya H Younis, Resident Assiut University

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Responsible Party: Aya Hasan, Resident in dermatology department, Assiut University
ClinicalTrials.gov Identifier: NCT03621995    
Other Study ID Numbers: OSITTBAAC
First Posted: August 9, 2018    Key Record Dates
Last Update Posted: July 16, 2019
Last Verified: July 2019
Additional relevant MeSH terms:
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Catalase
Antioxidants
Molecular Mechanisms of Pharmacological Action
Protective Agents
Physiological Effects of Drugs