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Evaluating the Live-Attenuated Human Parainfluenza Virus Type 3 Vectored Vaccine Candidate Expressing Ebolavirus Zaire Glycoprotein as the Sole Envelope Glycoprotein

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ClinicalTrials.gov Identifier: NCT03462004
Recruitment Status : Recruiting
First Posted : March 12, 2018
Last Update Posted : July 6, 2018
Sponsor:
Information provided by (Responsible Party):
National Institute of Allergy and Infectious Diseases (NIAID)

Brief Summary:
The purpose of this study is to evaluate the safety, infectivity, and immunogenicity of two doses of the HPIV3/ΔHNF/EbovZ GP vaccine candidate when administered intranasally in healthy adults.

Condition or disease Intervention/treatment Phase
Ebola Virus Disease Biological: HPIV3/ΔHNF/EbovZ GP vaccine Biological: Placebo Phase 1

Detailed Description:

This study will evaluate the safety, infectivity, and immunogenicity of two doses of the HPIV3/ΔHNF/EbovZ GP vaccine candidate when administered intranasally in healthy adults.

Participants will be enrolled sequentially in two cohorts. Participants in Cohort 1 will be randomly assigned to receive two doses of either 10^6.0 PFU/mL of HPIV3/ΔHNF/EbovZ-GP vaccine or placebo. The first dose will be given on Day 0 and the second dose will be given 4-8 weeks later. Participants in Cohort 2 will be randomly assigned to receive two doses of either 10^7.0 PFU/mL of HPIV3/ΔHNF/EbovZ-GP vaccine or placebo on Days 0 and 28.

Participants will be admitted to the inpatient unit 2 days prior to receiving their first dose of the vaccine or placebo. While in the inpatient unit, study procedures will include physical examinations, nasal washes, and blood collection. Participants will be discharged from the inpatient unit on Day 8 or thereafter once two consecutive rRT-PCR assay results are below the pre-determined threshold. An additional study visit will occur on Day 14.

On Day 26 (+28), participants will be readmitted to the inpatient unit, and will receive their second dose of the vaccine or placebo on Day 28 (+28). Participants will undergo the same study procedures that occurred during the first inpatient stay, and will be discharged 7 days after the second dose, or once the subject is without respiratory symptoms and rRT-PCR assay is below the pre-determined threshold (around Day 35). Additional study visits will occur on Days 42 ± 3, 56 ± 5, 84 ± 5, 112 ± 7, 180 ± 14, 270 ± 14, and 360 ± 30.


Study Type : Interventional  (Clinical Trial)
Estimated Enrollment : 30 participants
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
Primary Purpose: Prevention
Official Title: Phase 1 Evaluation of a Live-Attenuated Human Parainfluenza Virus Type 3 Vectored Vaccine Candidate Expressing Ebolavirus Zaire Glycoprotein as the Sole Envelope Glycoprotein
Actual Study Start Date : March 5, 2018
Estimated Primary Completion Date : October 2019
Estimated Study Completion Date : December 2019


Arm Intervention/treatment
Experimental: Cohort 1: HPIV3/ΔHNF/EbovZ GP vaccine
Participants will receive two doses of 10^6.0 PFU/mL of HPIV3/ΔHNF/EbovZ GP vaccine on Days 0 and 28 (+28).
Biological: HPIV3/ΔHNF/EbovZ GP vaccine
Administered intranasally

Placebo Comparator: Cohort 1: Placebo
Participants will receive two doses of placebo on Days 0 and 28 (+28).
Biological: Placebo
Administered intranasally

Experimental: Cohort 2: HPIV3/ΔHNF/EbovZ GP vaccine
Participants will receive two doses of 10^7.0 PFU/mL of HPIV3/ΔHNF/EbovZ GP vaccine on Days 0 and 28 (+28).
Biological: HPIV3/ΔHNF/EbovZ GP vaccine
Administered intranasally

Placebo Comparator: Cohort 2: Placebo
Participants will receive two doses of placebo on Days 0 and 28 (+28).
Biological: Placebo
Administered intranasally




Primary Outcome Measures :
  1. Frequency of vaccine-related solicited adverse events [ Time Frame: Measured through Day 35 ]
    Graded according to Division of AIDS (DAIDS) Table for Grading the Severity of Adult and Pediatric Adverse Events, Version 2.1, March 2017

  2. Area under the curve of nasal virus shedding after each dose of vaccine [ Time Frame: Measured through Day 35 ]
    Assessed by liquid titration of nasal secretions on LLC-MK2 monkey kidney cells

  3. Development of serum antibody to the EbovZ GP [ Time Frame: Measured through Day 360 ]
    As measured by enzyme-linked immunosorbent assay (ELISA)



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Ages Eligible for Study:   18 Years to 50 Years   (Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   Yes
Criteria

Inclusion Criteria:

  • Adult males and non-pregnant females between 18 and 50 years of age inclusive
  • General good health, without significant medical illness, physical examination findings, or significant laboratory abnormalities as determined by the investigator
  • Agree to storage of blood specimens for future research
  • Available for the duration of the trial
  • Able to demonstrate understanding of key study concepts, study rationale, and study participation requirements by scoring greater than or equal to 70% on a written comprehension assessment in less than or equal to 3 attempts.
  • Willingness to participate in the study as evidenced by signing the informed consent document
  • Female subjects of childbearing potential must agree to have used effective birth control methods beginning at least one month prior to vaccination, and continuing with 'per label/fully effective use' for the chosen method for the duration of the study, from amongst these:

    • pharmacologic/hormonal contraceptives, including oral, parenteral, subcutaneous, and transcutaneous delivery;
    • condoms with spermicide;
    • diaphragm with spermicide;
    • intrauterine device;
    • absolute abstinence from heterosexual intercourse as a matter of normal preferred lifestyle;
    • or must be surgically sterile, or must be age 50 AND have had no menses at all for at least one full year.
    • All females must provide samples for urine and serum pregnancy testing prior to enrollment (immediately prior to each vaccination), as well as a statement of menstrual history and a summary of all potentially reproductive sexual activity for the month prior to each vaccination, and at each study contact throughout the study, and report if they may be pregnant immediately.
  • Willingness to refrain from blood donation during the course of the study
  • Willingness to refrain from receiving other vaccines or investigational products during the first 4 months of the study after enrollment
  • Willingness to follow admission and isolation requirements for the indicated duration per protocol.

Exclusion Criteria:

  • Pregnancy as determined by a positive human choriogonadotropin (ß-HCG) test or history of recent unprotected intercourse in a woman of reproductive capacity
  • Currently breastfeeding
  • Evidence of clinically significant neurological, cardiac, pulmonary, hepatic, rheumatological, autoimmune, or renal disease by history, physical examination, and/or laboratory studies
  • History of intranasal pathology or evidence of structural abnormalities of the sinuses or nasal cavity upon examination
  • Behavioral or cognitive impairment or psychiatric disease that, in the opinion of the investigator, affects the subject's ability to understand and cooperate with the study protocol
  • Positive urine drug toxicology test indicating narcotic use or history of dependency
  • Have medical, occupational, or family problems as a result of alcohol or illicit drug use during the past 12 months
  • Other condition that, in the opinion of the investigator, would jeopardize the safety or rights of a subject participating in the trial, or would render the subject unable to comply with the protocol.
  • History of anaphylaxis
  • Current diagnosis of asthma or reactive airway disease (within the past 2 years)
  • Current history of allergic rhinitis requiring the use of medication in the 2 weeks before enrollment or the first 56 days of the study
  • History of Bell's palsy
  • Positive enzyme-linked immunosorbent assay (ELISA) and confirmatory test (e.g., Western blot or HIV-1/HIV-2 differentiation assay) for human immunodeficiency virus-1 (HIV-1)
  • Positive ELISA and confirmatory test (e.g., polymerase chain reaction (PCR) for virus) for hepatitis C virus (HCV)
  • Positive hepatitis B virus surface antigen (HBsAg) by ELISA test
  • Known immunodeficiency syndrome or history suggestive of impaired immune function
  • Use of corticosteroids (excluding topical preparations) or immunosuppressive drugs within 30 days prior to vaccination
  • Receipt of a live vaccine within 4 weeks or a killed vaccine within 2 weeks prior to study vaccination
  • History of asplenia.
  • Body mass index (BMI) less than 18.5 or greater than 40
  • Receipt of blood or blood-derived products (including immunoglobulin) within 6 months prior to study vaccination
  • Current tobacco user unwilling to refrain from smoking, snuffing, vaping, or chewing for the duration of the inpatient study (subjects may use nicotine patches if needed)
  • Travel to an Ebola-endemic region at the time when cases were present or planned travel to West or Central Africa during the study period
  • Receipt of another investigational vaccine or drug within 30 days prior to study vaccination
  • Previous receipt of an investigational Ebola or Marburg virus vaccine, a chimpanzee adenovirus, or human parainfluenza virus (HPIV)-vectored vaccine, or any other investigational vaccine likely to affect interpretation of the trial data
  • Current or past (in the last 4 weeks) user of intranasal medications (including steroids, decongestants, or hormonal medications), or planning to use them within 28 days of study vaccination
  • Live or have close contact with vulnerable individuals, including infants, the elderly, or immunocompromised individuals (those with HIV/AIDS, malignancy, or transplant patients)
  • History of Liver disease.

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT03462004


Locations
United States, Maryland
Center for Immunization Research, Johns Hopkins Bloomberg School of Public Health Recruiting
Baltimore, Maryland, United States, 21205
Contact: Rachel Adkinson    410-955-7283    radkins5@jhmi.edu   
Contact: Kawsar Talaat    410-502-9627    ktalaat@jhu.edu   
CIR Inpatient Unit, Johns Hopkins Bayview Medical Center Recruiting
Baltimore, Maryland, United States, 21224
Contact: Rachel Adkinson    410-955-7283    radkins5@jhmi.edu   
Contact: Kawsar Talaat    410-502-9627    ktalaat@jhu.edu   
Sponsors and Collaborators
National Institute of Allergy and Infectious Diseases (NIAID)
Investigators
Principal Investigator: Kawsar Talaat, MD Johns Hopkins Bloomberg School of Public Health

Responsible Party: National Institute of Allergy and Infectious Diseases (NIAID)
ClinicalTrials.gov Identifier: NCT03462004     History of Changes
Other Study ID Numbers: CIR 324
First Posted: March 12, 2018    Key Record Dates
Last Update Posted: July 6, 2018
Last Verified: July 2018

Studies a U.S. FDA-regulated Drug Product: Yes
Studies a U.S. FDA-regulated Device Product: No

Keywords provided by National Institute of Allergy and Infectious Diseases (NIAID):
Healthy Volunteer

Additional relevant MeSH terms:
Virus Diseases
Hemorrhagic Fever, Ebola
Hemorrhagic Fevers, Viral
RNA Virus Infections
Paramyxoviridae Infections
Filoviridae Infections
Mononegavirales Infections
Vaccines
Krestin
Immunologic Factors
Physiological Effects of Drugs
Adjuvants, Immunologic
Antibiotics, Antineoplastic
Antineoplastic Agents
Antiviral Agents
Anti-Infective Agents
Interferon Inducers
Radiation-Protective Agents
Protective Agents