Brown Adipose Tissue Activation: Effect of Exercise Training and Irisin (EXEBAT)

The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details. Identifier: NCT03359824
Recruitment Status : Completed
First Posted : December 2, 2017
Last Update Posted : December 13, 2017
Information provided by (Responsible Party):
Pirjo Nuutila, Turku University Hospital

Brief Summary:

This study investigates the brown adipose tissue (BAT) activation and metabolism using positron emission tomography (PET) imaging before and after exercise training intervention at fasting state under normal room temperature and during cold stimulation.The investigators hypothesize that BAT glucose uptake is increased after exercise and BAT becomes metabolically more active.

Understanding the mechanisms of BAT activation and the role of exercise in humans is crucial to combat epidemic of obesity and diabetes.

Condition or disease Intervention/treatment Phase
Physical Activity Other: Exercise Not Applicable

Detailed Description:

Exercise training has been suggested to activate and recruit human BAT through the activation of sympathetic nervous system and contracting skeletal muscles by the release of myokines such as lactate and irisin that signal WAT to induce expression of beige adipocytes. It has been also suggested that exercise training could enhance the metabolism of already existed functional BAT by increasing uncoupling protein-1 (UCP-1) gene transcription, mitochondrial biogenesis and hyperplasia. This is of clinical relevance because brown fat activation has been found to improve metabolic factors linked to obesity and diabetes.

In this study the investigators use a PET radiotracer 2-[18F] flouro-2-deoxy-D-glucose (FDG) to quantify the glucose uptake in BAT, whie adipose tissue and the muscle.The participants are scanned thrice at fast in this study once at room temperature, second at cold exposure and third after six weeks of exercise at cold exposure. This study will provide an insight about the role of exercise in activation of BAT metabolism.

Study Type : Interventional  (Clinical Trial)
Actual Enrollment : 11 participants
Intervention Model: Single Group Assignment
Masking: None (Open Label)
Primary Purpose: Basic Science
Official Title: Brown Adipose Tissue Activation: Effect of Exercise Training and Irisin
Actual Study Start Date : September 17, 2013
Actual Primary Completion Date : May 27, 2015
Actual Study Completion Date : May 27, 2015

Resource links provided by the National Library of Medicine

Arm Intervention/treatment
Arm: Exercise: Combination of moderate intensity continuous training, high intensity interval training and endurance training 5 times per week for a total of 6 weeks. Out of 5 sessions three were supervised by trainer and two sessions were performed by subjects on their own.The duration of the exercise was increased progressively. The first two weeks was 30 minutes that increased to 45 minutes in the third and fourth week. It was 60 minutes for the last two weeks.
Other: Exercise

Primary Outcome Measures :
  1. Cold-induced glucose uptake in BAT after exercise [ Time Frame: Within one study day ]
    Glucose uptake of brown adipose tissue acquired with PET under cold exposure after exercise intervention. PET imaging is used to quantify glucose uptake in tissues

Information from the National Library of Medicine

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Ages Eligible for Study:   18 Years to 35 Years   (Adult)
Sexes Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes

Inclusion Criteria:

  • Healthy sedentary volunteer

    1. BMI 20-25 kg/m2
    2. Age: 18-35 years
    3. No chronic diseases

Exclusion Criteria:

  1. BMI < 20 kg/m2 or BMI > 25 kg/m2
  2. Mental disorder or poor compliance
  3. Eating disorder or excessive use of alcohol or tobacco smoking
  4. Any chronic disease
  5. Past dose of radiation
  6. Presence of any ferromagnetic objects that would make MR imaging contraindicated
  7. Physically disability which rule out the physical fitness tests or participation in the exercise training intervention
  8. Any other condition that in the opinion of the investigator could create a hazard to the subject safety, endanger the study procedures or interfere with the interpretation of study results

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its identifier (NCT number): NCT03359824

Sponsors and Collaborators
Turku University Hospital
Principal Investigator: Pirjo Nuutila, MD, PhD Turku UH

Responsible Party: Pirjo Nuutila, MD., Ph.D., Turku University Hospital Identifier: NCT03359824     History of Changes
Other Study ID Numbers: EXEBAT
First Posted: December 2, 2017    Key Record Dates
Last Update Posted: December 13, 2017
Last Verified: December 2017
Individual Participant Data (IPD) Sharing Statement:
Plan to Share IPD: No

Studies a U.S. FDA-regulated Drug Product: No
Studies a U.S. FDA-regulated Device Product: No

Keywords provided by Pirjo Nuutila, Turku University Hospital:
exercise, brown adipose tissue