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Efficacy of Vitamin A Fortified Rice in Lactating Thai Women (EVAL)

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ClinicalTrials.gov Identifier: NCT03056625
Recruitment Status : Recruiting
First Posted : February 17, 2017
Last Update Posted : September 20, 2017
Sponsor:
Information provided by (Responsible Party):
Siwaporn Pinkaew, Prince of Songkla University

Brief Summary:

Vitamin A deficiency (VAD) remains a public health problem that occurs in children and women. WHO estimates that 190 million preschool age children (under five years of age) and 19.1 million pregnant women have inadequate concentrations of retinol . VAD may increase risk of morbidity and mortality during childhood, pregnancy and postpartum period . In developing countries, vitamin A status in lactating women is not optimal. Even in the United States, vitamin A status of low income pregnant women is marginal. Therefore, women of reproductive age are at risk of vitamin A depletion which compromises the vitamin A status of breastfed infants. Vitamin A is transferred to milk from both retinol binding protein bound and chylomicron associated vitamin A carriers. Because of teratogenicity of vitamin A, the transfer of vitamin A from pregnant mother to the fetus is carefully controlled. This results in all infants born with very low stores of vitamin A . Therefore human milk is still the best vitamin A source for infants. The concentration of vitamin A in breast milk is highest in the first 21 days postpartum (colostrum in the first 4-6 days and transitional milk in the next 7-21 days). Breast milk vitamin A concentrations and an infant's vitamin A status are based on maternal dietary intake. Breast milk retinol concentrations are a useful tool and a unique indicator for lactating women and represents extrapolations of vitamin A status of breastfed infant.

In high risk vitamin A deficiency areas, prior recommendation for lactating women was to give two doses of 200,000 IU vitamin A within 6 weeks post-delivery . However, the lack of impact evidence of this regimen led to the WHO's withdrawal of such recommendation as a public health policy .

Food-based intervention has been regarded as a sustainable approach to improve population vitamin A status. Most recently, vitamin A-depleted sows fed high-provitamin A carotenoid maize resulted in significant increase in liver stores in nursing piglets and significant higher milk retinol concentrations than sows fed white maize that given a high-dose vitamin A supplement .

Previous efforts to fortify staples or common vehicles with vitamin A offer promising solution to prevention of vitamin A deficiency in vulnerable population . Food fortified with vitamin A has potentials to improve women's vitamin A status and to increase the vitamin A concentrations of breast milk . Rice as the most important staple food in Thailand, represents the culture of consumption and lifestyle of Thai people . Recent study in Thai school children fed with extruded rice grains fortified with zinc, iron and vitamin A every weekday for 2 months, significantly increased liver stores of vitamin A, as assessed with a stable isotope technique . Likewise, fortification of rice with vitamin A may also benefit mothers during lactation.

The outcomes of this study will be useful to guide the promotion of food-based strategies to improve vitamin A status of mother and infant during lactation.


Condition or disease Intervention/treatment Phase
Vitamin A Status Other: Vitamin A fortified rice Other: Normal rice Not Applicable

Detailed Description:

Objectives To determine the efficacy of vitamin A fortified rice that gets into the breast milk of lactating mothers as determined by breast milk vitamin A concentrations, total body reserves of vitamin A via isotope dilution technique, following a 14 weeks feeding trial of lactating women.

Methodology The intervention will have two arms, vitamin A fortified rice and regular rice with 35 participants (lactating women) per group. Participants will receive one meal per day on weekdays for a period of 14 weeks. The fortified dose will be measured in the final cooked product, but we will aim to administer approximately 500 µg retinol in the rice meal. Vitamin A fortified rice is produced by extrusion technology by adding retinol into an artificial rice to be mix with the normal rice.

Data collection

  1. Demographic data Each participants will be asked about demographic data in the first interview. The data includes general information (age, education and occupation), socioeconomic status, number of child and duration of breastfeeding mothers, if women had a child before.
  2. Anthropometric data Anthropometric measurements include weight and height of participants and weight, length and head circumference of infants will be recorded in the first day and post-intervention.
  3. Dietary assessment At the baseline (Day 1: 3+1 week after first breastfeed), all participants will be interviewed on food consumption in the previous 24 hours for 3 times (2 weekdays and 1 weekend day). The data consist of food items, food types, food components and weight or size of consumed portions. Information from the interviews will be analyzed by INMUCAL software (Mahidol University, Thailand)
  4. Collection and analysis of breast milk sample Breast milk samples will be collected for vitamin A analysis. Collection of breast milk sample will be divided into two periods at the following time points which will start 3+1 week after first breastfeed.

    • at baseline: Day 1 and 15
    • 14 weeks post-intervention: Day 121 and 135
  5. Collection and analysis of blood sample The change of vitamin A status of participants will be determined by using the pair13C-retinol isotope dilution test . This involves giving the first dose of 2 µmol retinol equivalent of 13C2-retinolretinyl acetate to participants on day 1 after that the second dose (2 µmol retinol) will be given to participants on the day 121. Blood sample will be collected 4 times (Day 1, 15, 121 and 135 after 3+1 week of first breastfeed) for measuring the isotopic ratio of 13C/12C and the calculation of liver vitamin A concentration. Blood samples will be drawn into plastic clot blood tubes and will be wrapped with aluminum foil for protection from light. Then, blood will be centrifuged at 3000 rpm for 15 minutes, serum will be separated immediately into dark cryovials and stored at -20 ◦c.

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Study Type : Interventional  (Clinical Trial)
Estimated Enrollment : 70 participants
Allocation: Randomized
Intervention Model: Parallel Assignment
Masking: Double (Participant, Investigator)
Primary Purpose: Prevention
Official Title: Efficacy of Vitamin A Fortified Rice in Lactating Thai Women
Actual Study Start Date : March 15, 2017
Actual Primary Completion Date : August 31, 2017
Estimated Study Completion Date : December 24, 2017

Resource links provided by the National Library of Medicine

MedlinePlus related topics: Vitamin A

Arm Intervention/treatment
Experimental: Fortified rice
Participant will be given vitamin A fortified rice 1 meal/day
Other: Vitamin A fortified rice
Lactating women will receive fortified rice one meal per day on weekdays for a period of 14 weeks.

Placebo Comparator: Control
Participant will be given normal rice 1 meal/day
Other: Normal rice
Lactating women will receive normal rice one meal per day on weekdays for a period of 14 weeks.




Primary Outcome Measures :
  1. Vitamin A liver store [ Time Frame: 135 days ]
    vitamin A concentration per gram liver


Secondary Outcome Measures :
  1. Breast milk retinol [ Time Frame: 135 days ]
    vitamin A (retinol) concentration in breast milk

  2. Serum retinol [ Time Frame: 121 days ]
    Concentration of retinol in serum


Other Outcome Measures:
  1. Hb [ Time Frame: 135 days ]
    Hemoglobin concentration in whole blood

  2. The gut microbiome [ Time Frame: 135 days ]
    Changing of the composition of the microbiota



Information from the National Library of Medicine

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Ages Eligible for Study:   18 Years to 38 Years   (Adult)
Sexes Eligible for Study:   Female
Gender Based Eligibility:   Yes
Gender Eligibility Description:   lactation women
Accepts Healthy Volunteers:   Yes
Criteria

Inclusion Criteria:

  • free of diseases
  • gestational age between 37 to 42 weeks
  • willing to breastfeed for a period of 4-6 months

Exclusion Criteria:

  • Hb > 70 g/L
  • Postpartum Hemorrhage
  • Twin, Baby with low birth weight
  • Receiving vitamin A supplement during the study

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT03056625


Contacts
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Contact: Siwaporn Pinkaew, Ph.D +66899772266 siwaporn.p@psu.ac.th

Locations
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Thailand
Prince of Songkla University Recruiting
Pattani, Thailand, 94000
Contact: Siwaporn Pinkaew, Ph.D    899772266    siwaporn.p@psu.ac.th   
Sponsors and Collaborators
Prince of Songkla University
Investigators
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Principal Investigator: Siwaporn Pinkaew, Ph.D Prince of Songkla University

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Responsible Party: Siwaporn Pinkaew, Principal investigator, Prince of Songkla University
ClinicalTrials.gov Identifier: NCT03056625     History of Changes
Other Study ID Numbers: MGR5980155
First Posted: February 17, 2017    Key Record Dates
Last Update Posted: September 20, 2017
Last Verified: September 2017
Individual Participant Data (IPD) Sharing Statement:
Plan to Share IPD: No

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Studies a U.S. FDA-regulated Drug Product: No
Studies a U.S. FDA-regulated Device Product: No

Keywords provided by Siwaporn Pinkaew, Prince of Songkla University:
fortified rice
lactating women
13C2-retinol isotope dilution
vitamin A liver store

Additional relevant MeSH terms:
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Vitamins
Vitamin A
Retinol palmitate
Micronutrients
Nutrients
Growth Substances
Physiological Effects of Drugs
Antioxidants
Molecular Mechanisms of Pharmacological Action
Protective Agents
Anticarcinogenic Agents
Antineoplastic Agents