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Antimicrobial Peptides in Periodontitis (PAROPAM)

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ClinicalTrials.gov Identifier: NCT02793453
Recruitment Status : Completed
First Posted : June 8, 2016
Last Update Posted : March 30, 2017
Sponsor:
Information provided by (Responsible Party):
CHU de Reims

Brief Summary:
Periodontal diseases are complex chronic pathologies presenting a high prevalence worldwide. Etiopathological process involved in initiation and progression of these multifactorial diseases are not well understood, explaining the huge clinical forms variability and the complexity of the currently used classification of periodontal diseases. Recently, the implication of antimicrobial peptides (AMPs) have been suggested as potential track of explanation for periodontal diseases pathogeny. The here presented study explore the level of expression of genes coding those peptides by studying periodontal smears obtained from healthy and periodontitis patients.

Condition or disease Intervention/treatment Phase
Periodontal Disease : Chronic Periodontitis Other: periodontal smears Not Applicable

Detailed Description:
This study explore the level of expression of genes coding those peptides by studying Messenger RNA (mRNA) purified from periodontal smears obtained from healthy and periodontitis patients by "Masterpure©" protocol.After reverse transcription (RT-PCR), in-silico design of primers and specificity and efficiency calculation, Complementary DNA (cDNA) are quantified by a semi-quantitative method (delta-delta CT) vs a Housekeeping gene. Wilcoxon-Mann & Whitney tests and Chi² tests are performed to check the significativity of the results between groups.

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Study Type : Interventional  (Clinical Trial)
Actual Enrollment : 29 participants
Allocation: Non-Randomized
Intervention Model: Parallel Assignment
Masking: Single (Investigator)
Primary Purpose: Diagnostic
Official Title: Antimicrobial Peptides in Periodontitis : a Pilot Study
Actual Study Start Date : January 19, 2016
Actual Primary Completion Date : March 23, 2016
Actual Study Completion Date : April 2016

Resource links provided by the National Library of Medicine

MedlinePlus related topics: Antibiotics

Arm Intervention/treatment
Diseased
Patients suffering of moderate to severe chronic periodontitis
Other: periodontal smears
Healthy
Healthy Patients not suffering of any periodontal disease a
Other: periodontal smears



Primary Outcome Measures :
  1. Antimicrobial peptides expression levels [ Time Frame: Day 0 ]
    Level of expression of antimicrobial peptides assessed by RT-PCR


Secondary Outcome Measures :
  1. mRNA quantity [ Time Frame: Day 0 ]
    Quantity of mRNA purified on the smears



Information from the National Library of Medicine

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Ages Eligible for Study:   35 Years to 65 Years   (Adult, Older Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   No
Criteria

Inclusion Criteria:

  • Patients suffering of moderate to severe chronic periodontitis as described by the classification Armitage-1999, aged 35-65yo

Exclusion Criteria:

  • Pregnant females, patients suffered from any other systemic diseases (cardiovascular, pulmonary, liver, cerebral, diseases or diabetes, cancer)
  • patients that had received antibiotic treatment in the previous 3 months or who were taking long-term anti-inflammatory drugs; who had received a course of periodontal treatment or orthodontics treatment within the last 6 months; who were heavy smokers (>5 cig/day)

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT02793453


Locations
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France
Chu de Reims
Reims, France, 51092
Sponsors and Collaborators
CHU de Reims

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Responsible Party: CHU de Reims
ClinicalTrials.gov Identifier: NCT02793453     History of Changes
Other Study ID Numbers: PO15100
First Posted: June 8, 2016    Key Record Dates
Last Update Posted: March 30, 2017
Last Verified: March 2017

Additional relevant MeSH terms:
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Periodontitis
Chronic Periodontitis
Periodontal Diseases
Mouth Diseases
Stomatognathic Diseases
Anti-Infective Agents
Anti-Bacterial Agents