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Impact of Ageing on Adipose, Muscle and Systemic Inflammation

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ClinicalTrials.gov Identifier: NCT02777138
Recruitment Status : Active, not recruiting
First Posted : May 19, 2016
Last Update Posted : August 24, 2018
Sponsor:
Information provided by (Responsible Party):
William Trim, University of Bath

Brief Summary:
The accumulation and dysfunction of excess adipose (fat) tissue that occurs with ageing is associated with a number of chronic inflammatory disorders such as type 2 diabetes and cardiovascular disease but the underlying mechanisms are not understood.

Condition or disease Intervention/treatment
Inflammation Aging Other: Maintaining a normal daily living lifestyle

  Show Detailed Description

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Study Type : Observational
Estimated Enrollment : 24 participants
Observational Model: Other
Time Perspective: Cross-Sectional
Official Title: Impact of Ageing on Adipose, Muscle and Systemic Inflammation
Study Start Date : May 2016
Actual Primary Completion Date : March 2018
Estimated Study Completion Date : October 2018

Group/Cohort Intervention/treatment
Young males aged 20-35 years old

Maintaining a normal daily living lifestyle

  • Healthy
  • Reasonably active- PAL: 1.4-1.9
  • Non-obese- Fat mass index based on DEXA of 4-8kg/m2
  • Weight stable for more than 3 months (±3% body mass)
  • Non-smoker
  • No chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, peripheral arterial disease, insulin- or non-insulin dependent diabetes or other metabolic disorders
  • No daily consumption of analgesic or anti-inflammatory drug(s) including NSAIDs and corticosteroids, prescription or non-prescription
  • No medications that may influence lipid or carbohydrate metabolism or immune system function
  • No known negative reaction to lidocaine
  • No participation in heavy resistance training
Other: Maintaining a normal daily living lifestyle
participants will undertake a normal lifestyle with no alterations to daily living in order to access the differences in tissue (muscle and adipose) and systemic inflammation between the two age cohorts

Old males aged 65-85 years old

Maintaining a normal daily living lifestyle

  • Healthy
  • Reasonably active- PAL: 1.4-1.9
  • Non-obese- Fat mass index based on DEXA of 4-8kg/m2
  • Weight stable for more than 3 months (±3% body mass)
  • Non-smoker
  • No chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, peripheral arterial disease, insulin- or non-insulin dependent diabetes or other metabolic disorders
  • No daily consumption of analgesic or anti-inflammatory drug(s) including NSAIDs and corticosteroids, prescription or non-prescription
  • No medications that may influence lipid or carbohydrate metabolism or immune system function
  • No known negative reaction to lidocaine
  • No participation in heavy resistance training
Other: Maintaining a normal daily living lifestyle
participants will undertake a normal lifestyle with no alterations to daily living in order to access the differences in tissue (muscle and adipose) and systemic inflammation between the two age cohorts




Primary Outcome Measures :
  1. Differences in immune cell populations (macrophages/Tcells) in adipose tissue of young versus old males [ Time Frame: through to study completion, an average of 14 months ]

Secondary Outcome Measures :
  1. the impact of ageing on mRNA expression of key metabolic and inflammatory genes in adipose tissue. [ Time Frame: through to study completion, an average of 14 months ]
  2. the impact of ageing on metabolic and inflammatory protein secretions (into culture media) from adipose tissue [ Time Frame: through to study completion, an average of 14 months ]
  3. differences in immune cell populations in muscle tissue and blood compared to adipose tissue from the same individuals and the impact of ageing on immune cell populations in muscle tissue. [ Time Frame: through to study completion, an average of 14 months ]
  4. the impact of ageing on mRNA expression of other key metabolic and inflammatory genes in muscle tissue. [ Time Frame: through to study completion, an average of 14 months ]
  5. activation/inhibition of e.g. insulin stimulated pathways in adipose and muscle tissues (for example assessment of Akt/IRS1 phosphorylation by western blot analysis). [ Time Frame: through to study completion, an average of 14 months ]

Biospecimen Retention:   Samples With DNA
muscle and adipose biopsies blood plasma blood serum peripheral blood mononuclear cells


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Ages Eligible for Study:   20 Years to 85 Years   (Adult, Older Adult)
Sexes Eligible for Study:   Male
Accepts Healthy Volunteers:   Yes
Sampling Method:   Non-Probability Sample
Study Population
general free-living public in the Bath and the surrounding areas
Criteria

Inclusion Criteria:

  • Male
  • Aged between 20-35 years (Group 1) or 65-85 years (Group 2)
  • Fat mass index based on DEXA between 4-8 kg/m2
  • Weight stable for more than 3 months (no change in weight +/- 3%)
  • Physical Activity Level (PAL) between 1.4 and 1.9
  • Non-smoker
  • Healthy

Exclusion Criteria:

  • Any chronic illness, cardiac, pulmonary, liver, or kidney abnormalities, uncontrolled hypertension, peripheral arterial disease, insulin- or non-insulin dependent diabetes or other metabolic disorders
  • Individuals who consume on a daily basis any analgesic or anti-inflammatory drug(s) including NSAIDs and corticosteroids, prescription or non-prescription
  • Taking any medications that may influence lipid or carbohydrate metabolism or immune system function
  • Individuals with a known negative reaction to lidocaine anaesthetic
  • Participation in heavy resistance training

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT02777138


Locations
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United Kingdom
university of bath - Department for Health
Bath, Somerset, United Kingdom, BA2 7AY
Sponsors and Collaborators
University of Bath
Investigators
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Principal Investigator: Dylan Thompson, Professor University of Bath

Publications:

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Responsible Party: William Trim, Ph.D. Student, University of Bath
ClinicalTrials.gov Identifier: NCT02777138     History of Changes
Other Study ID Numbers: 16/sw/0003
First Posted: May 19, 2016    Key Record Dates
Last Update Posted: August 24, 2018
Last Verified: August 2018
Individual Participant Data (IPD) Sharing Statement:
Plan to Share IPD: Yes

Keywords provided by William Trim, University of Bath:
Adipose tissue
Inflammation
Age Comparison
Macrophages
T-lymphocytes
Adipose Progenitors

Additional relevant MeSH terms:
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Inflammation
Pathologic Processes