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Study of Free DNA Content of Embryo Culture Medium as a Non-Invasive Tool for Embryo Assessment

This study has been completed.
Sponsor:
ClinicalTrials.gov Identifier:
NCT02480491
First Posted: June 24, 2015
Last Update Posted: June 24, 2015
The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details.
Collaborator:
Ain Shams University
Information provided by (Responsible Party):
Azza Awad Abdel Razik Mohamed, Sadat City University
  Purpose
Study of Cell Free DNA Content of Human Embryo Culture Medium as a Non-Invasive Tool for Embryo Assessment in intra cytoplasmic sperm injection Treatment Cycles.

Condition Intervention
Infertility Other: measuring cell freeDNA

Study Type: Observational
Study Design: Observational Model: Cohort
Official Title: Study of Cell Free DNA Content of Human Embryo Culture Medium as a Non-Invasive Tool for Embryo Assessment in ICSI Treatment Cycles

Further study details as provided by Azza Awad Abdel Razik Mohamed, Sadat City University:

Primary Outcome Measures:
  • correlations between levels of measured cell free DNA and embryo quality. [ Time Frame: 12 months ]

Biospecimen Retention:   Samples With DNA
cell free DNA content of the culture medium of the human embryo

Enrollment: 100
Study Start Date: January 2014
Study Completion Date: May 2015
Primary Completion Date: February 2015 (Final data collection date for primary outcome measure)
Groups/Cohorts Assigned Interventions
Third day embryos
embryos culture media during third day after fertilization
Other: measuring cell freeDNA
measuring cell free DNA using PCR
Fifth day embryos
embryos culture media during fifth day after fertilization
Other: measuring cell freeDNA
measuring cell free DNA using PCR

Detailed Description:
The aim of the present study is to determine whether cell free DNA content of the culture medium of the human embryo correlates with the quality and reproductive potential of embryos in ICSI cycles and, thus, to look for a new non invasive tool of assessing embryo quality beside morphological assessment. Determining a rapid, noninvasive method for the assessment of the developmental potential of embryos may be a great achievement
  Eligibility

Information from the National Library of Medicine

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Ages Eligible for Study:   Child, Adult, Senior
Sexes Eligible for Study:   Female
Accepts Healthy Volunteers:   Yes
Sampling Method:   Non-Probability Sample
Study Population
ICSI Treatment Cycles females
Criteria

Inclusion Criteria:

  • third day embryos

Exclusion Criteria:

  • less than third day embryos
  Contacts and Locations
No Contacts or Locations Provided
  More Information

Responsible Party: Azza Awad Abdel Razik Mohamed, Director of Embryology Lab., Ain Shams Univesity, Sadat City University
ClinicalTrials.gov Identifier: NCT02480491     History of Changes
Other Study ID Numbers: SCS-1
First Submitted: June 20, 2015
First Posted: June 24, 2015
Last Update Posted: June 24, 2015
Last Verified: June 2015

Additional relevant MeSH terms:
Infertility
Genital Diseases, Male
Genital Diseases, Female