Role of Endoplasmic Reticulum Stress in the Pathophysiology of Type 2 Diabetes (GLUCOSTRESS)
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|ClinicalTrials.gov Identifier: NCT02368704|
Recruitment Status : Unknown
Verified April 2016 by Assistance Publique - Hôpitaux de Paris.
Recruitment status was: Active, not recruiting
First Posted : February 23, 2015
Last Update Posted : April 18, 2016
The purpose of this study is to investigate whether Endoplasmic Reticulum (ER) stress pathway is activated in peripheral tissues (adipose tissue) in insulin resistant diabetic patients compared to healthy subjects normoglycemic matched for age and sex an to investigate whether ER stress pathway can be activated in response to insulin. Indeed, some preliminar on rates studies shows that ER stress pathway is activated by insulin in liver and adipose tissue showing that hyperinsulinemia might help trigger stress path ER.
For this, we propose a case control study of type 2 diabetic patients vs control subjects in which markers of ER stress will be evaluated from abdominal subcutaneous adipose tissue obtained before and after euglycemic hyperinsulinemic. We chose to consider adipose tissue subcutaneous rather than visceral adipose tissue for obvious reasons of lesser invasiveness.
|Condition or disease||Intervention/treatment|
|Diabetes Mellitus, Type 2 Endoplasmic Reticulum Stress||Other: No intervention|
|Study Type :||Observational|
|Actual Enrollment :||40 participants|
|Observational Model:||Case Control|
|Official Title:||Role of Endoplasmic Reticulum Stress in the Pathophysiology of Type 2 Diabetes|
|Study Start Date :||November 2012|
|Estimated Primary Completion Date :||November 2016|
|Estimated Study Completion Date :||November 2016|
control subjects with :
Other: No intervention
Diabetic patients with :
Other: No intervention
- Show an increase in markers of ER stress in fasting patients with type 2 diabetes [ Time Frame: 2 days ]Protein expression markers of ER stress pathway BiP / GRP 78, CHOP, ATF4 EDEM and XBP-1
- Assess whether insulin induces an increase in markers of ER stress [ Time Frame: 2 days ]Insulin sensitivity
- Insulin secretion in response to glucose infusion [ Time Frame: 2 days ]
- Insulin secretion in response to arginine infusion [ Time Frame: 2 days ]
- Lipolysis on insulin [ Time Frame: 2 days ]
Biospecimen Retention: Samples With DNA
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Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT02368704
|Paris, France, 75010|