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Immunolocalization of 1,25,Dihydroxyvitamin D3 in Aggressive Periodontitis Patients (IVDAP)

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ClinicalTrials.gov Identifier: NCT02282800
Recruitment Status : Completed
First Posted : November 4, 2014
Last Update Posted : November 4, 2014
Sponsor:
Information provided by (Responsible Party):
Dr.Dasari Rajashre, Panineeya Mahavidyalaya Institute of Dental Sciences & Research Centre

Brief Summary:
The study was to intended to visualize the nuclear localization of vitamin D receptor in oral tissues of aggressive periodontitis patients to better understand the potential for receptor in disease activity or progression.

Condition or disease Intervention/treatment
Aggressive Periodontitis Genetic: Vitamin D receptor

Detailed Description:

Aggressive periodontitis (AgP) is an infectious disease of the periodontium which affects young adults, causing damage to the supporting apparatus of the teeth, leading to rapid bone resorption and tooth loss. Its clinical features have been shown to result from several genetic and environmental factors.

This has increased the interest to research those genetic host factors as they may provide useful risk markers, reveal important information regarding the pathogenesis and also identification of effective preventive agents that might help decrease the disease burden.

1,25-dihydroxyvitamin D3,apart from these traditional calcium-related actions, this active form is being increasingly recognized for its potent anti-proliferative, anti-differentiative and immunomodulatory activities.

The calcitriol activity is largely mediated by the vitamin D receptor (VDR) whose expression has been described in some tissues. VDR is localized in both the cytosol and nucleus and accumulates in the nucleus in response to 1,25,dihydroxyvitamin D3 binding.

Expression and nuclear activation of the VDR are necessary for the effects of vitamin D. Thus, alterations in the vitamin D pathway lead to disturbances in mineral metabolism. It may be hypothesized that the rapid bone loss which occurs in aggressive periodontitis patients may be due to decreased or absence of expression of Vitamin D receptor in the nucleus.


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Study Type : Observational
Actual Enrollment : 61 participants
Observational Model: Case Control
Time Perspective: Cross-Sectional
Official Title: Immunolocalization of 1,25,Dihydroxyvitamin D3 in Aggressive Periodontitis Patients
Study Start Date : November 2010
Actual Primary Completion Date : August 2011
Actual Study Completion Date : December 2011

Resource links provided by the National Library of Medicine

MedlinePlus related topics: Vitamin D
Drug Information available for: Vitamin D

Group/Cohort Intervention/treatment
Case group
Case group include patients with generalized AgP were the tissue samples taken to analyse the number of vitamin D receptor present.
Genetic: Vitamin D receptor
50-100mg gingival tissue samples were collected from both test and control group for immunohistochemical staining to assess the number of vitamin D receptors in nucleus as well cytoplasm and also for RNA isolation to check the presence of VDR gene.
Other Name: 1,25,dihydroxyvitamin D3 receptor

Control group
Ethnically matched, systemically and periodontally healthy individuals were included in control group were also gingival tissue taken for analysis of number of receptors present in nucleus and cytoplasm
Genetic: Vitamin D receptor
50-100mg gingival tissue samples were collected from both test and control group for immunohistochemical staining to assess the number of vitamin D receptors in nucleus as well cytoplasm and also for RNA isolation to check the presence of VDR gene.
Other Name: 1,25,dihydroxyvitamin D3 receptor




Primary Outcome Measures :
  1. Less nuclear receptors in aggressive periodontitis [ Time Frame: 24hrs ]
    Immunohistochemical staining has been done for test group and control group tissue samples where less nuclear vitamin D receptors are found in aggressive periodontitis patients.



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Ages Eligible for Study:   24 Years to 32 Years   (Adult)
Sexes Eligible for Study:   All
Accepts Healthy Volunteers:   No
Sampling Method:   Non-Probability Sample
Study Population
The study population was divided in to two groups i.e test and control group
Criteria

Inclusion Criteria:

  1. Generalized Aggressive periodontitis patients were onset of periodontal disease at <35yrs of age and atleast eight teeth with probing depth >6mm and radiographic evidence of alveolar bone loss, three of which were not first molar or incisor under test group.
  2. Control group includes systemically and periodontally healthy individuals who is of same age as that of test group.

    -

Exclusion Criteria

  1. Subjects taking drugs for vitamin D or calcium
  2. Subjects received periodontal therapy within previous year
  3. Pregnant subjects
  4. Smokers
  5. Patients with systemic diseases -

Information from the National Library of Medicine

To learn more about this study, you or your doctor may contact the study research staff using the contact information provided by the sponsor.

Please refer to this study by its ClinicalTrials.gov identifier (NCT number): NCT02282800


Sponsors and Collaborators
Panineeya Mahavidyalaya Institute of Dental Sciences & Research Centre
Investigators
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Principal Investigator: Rajashree Dasari, MDS Panineeya Mahavidyalaya

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Responsible Party: Dr.Dasari Rajashre, Immunolocalization of 1,25,Dihydroxyvitamin D3 in Aggressive Periodontitis Patients, Panineeya Mahavidyalaya Institute of Dental Sciences & Research Centre
ClinicalTrials.gov Identifier: NCT02282800     History of Changes
Other Study ID Numbers: PanineeyaMVIDS
First Posted: November 4, 2014    Key Record Dates
Last Update Posted: November 4, 2014
Last Verified: October 2014

Additional relevant MeSH terms:
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Periodontitis
Aggression
Aggressive Periodontitis
Periodontal Diseases
Mouth Diseases
Stomatognathic Diseases
Behavioral Symptoms
Vitamins
Vitamin D
Ergocalciferols
Dihydroxycholecalciferols
Micronutrients
Nutrients
Growth Substances
Physiological Effects of Drugs
Bone Density Conservation Agents
Calcium-Regulating Hormones and Agents