Role of Metabolic Enzymes in Non-Melanoma Skin Cancer
|Study Design:||Observational Model: Case-Only
Time Perspective: Prospective
|Official Title:||Role of Metabolic Enzymes in Non-Melanoma Skin Cancer: Potential for Informative Biomarkers and Therapeutic Targets|
- expression levels of metabolic enzymes in normal skin versus precancerous lesions or skin cancer [ Time Frame: 1 month ]
Overall, compare normal skin versus precancerous lesions or skin cancer from the same individual. Paired tests of samples are selected from the same subjects.
- 10 actinic keratosis samples,
- 10 basal cell carcinoma samples
- 20 squamous cell carcinoma samples
- Normal skin control biopsy from the same subject will be collected.
|Study Start Date:||March 2011|
|Study Completion Date:||August 2015|
|Primary Completion Date:||August 2015 (Final data collection date for primary outcome measure)|
Other: skin specimen
Biopsy procedure will be done at the Beckman Laser Institute or at the Dermatology Clinic Gottschalk Medical Plaza, of University of California, Irvine. Standard procedure of punch biopsy will be done in the areas determined by the physician researchers. The normal skin biopsy will be obtained from an area of skin without any suspicious lesions located on an area of the body. Biopsy specimens will be stored frozen at Beckman Laser Institute chemistry-lab for research and will be assigned with subject ID number, there will be no personnel health information attach to the skin specimens. There will be no cost to the subject or their insurer for participation in this study, except in the specific scenario of a suspected skin cancer that would undergo biopsy regardless of participation in this study. The time commitment for each subject is approximately 1 hour for each set of biopsies.
Researchers can test the hypothesis that expression of key metabolic enzymes is reduced and/or lost during skin cancer progression. Total ribonucleic acid, a single-stranded biologic molecule involved in gene transcription, regulation, and translation, can be isolated from biopsied skin specimens. Quantitative Real-Time can be performed to determine the expression levels of several key metabolic enzymes.
Metabolic enzymes regulate several vital signaling pathways in the human body. Some examples include hormone and bioactive lipid regulation. Aberrant regulation of these signaling pathways can lead to human diseases including cancer.
Recent evidence has shown that metabolic enzyme call UGT in the genes call UDP-glucuronosyltransferases were shown to have significantly reduced expression in breast tumors compared with normal breast tissue from the same patients This observed loss of expression is hypothesized to be causal as these two enzymes are responsible for estrogen metabolism.
However, relatively little is known about metabolic enzymes and skin cancer. We have preliminary data that UGT enzyme expression is lost during melanoma progression which allows deregulated lipoxygenase signaling.
Please refer to this study by its ClinicalTrials.gov identifier: NCT02244749
|United States, California|
|Beckman Laser Institute|
|Irvine, California, United States, 92612|
|Gottschalk Medical Plaza, 1 Medical Plaza Drive|
|Irvine, California, United States, 92697|
|Principal Investigator:||Kristen Kelly, MD||Beckman Laser Institute, UCI|