Modulation of Muscle Protein Synthesis With Diet and Exercise in Old Aged Women
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In this study will measure differences in how muscle responds to both exercise and protein supplements in healthy women aged 60-69. We are studying two different protein supplements- a standard Whey Protein and a Leucine enriched supplement. Each patient would receive either one of these.
Condition or disease
One of the factors that plays an important role in the loss of functional performance and, as such, the capacity to maintain a healthy, active lifestyle is the progressive loss of skeletal muscle mass with ageing (i.e., sarcopenia). Indeed, sarcopenia is a more robust predictor of functional status and mortality in the elderly than chronological age or indeed, any other co-morbidity. Sarcopenia is an incipient process whereby lean muscle mass contributing up to ~50% of total body weight in young adults declines to ~25% when reaching the age of 75-80 y. Adequate nutritional intake, and in particular dietary protein, is important for offsetting age-related declines in muscle mass. The aim of this project is to seek the most effective nutrition with which to counteract sarcopenia, specifically in women.
Muscle protein synthesis and muscle protein breakdown [ Time Frame: 7 Hours ]
Markers of muscle protein synthesis and breakdown are determined from muscle biopsy samples in form of myofibrillar Fractional Synthetic Rate (FSR) assessed by gas-chromatography-combustion-mass spectrometry, using incorporation of a stable isotope tracer (13-C-6 Phe)
Secondary Outcome Measures :
Blood flow (bulk and nutritive) and intramuscular cell signalling [ Time Frame: 7 Hours ]
Leg blood flow via Common femoral bulk flow (Doppler ultrasound)
Microvascular blood flow via contrast enhanced ultrasound
Mammalian Target of Rapamycin-1 (mTORC1) and Protein Kinase B (PKB or AKT) phosphorylation via western immunoblotting
Biospecimen Retention: Samples With DNA
Blood samples for Glucose and RNA analysis Muscle biopsies for protein synthesis
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Layout table for eligibility information
Ages Eligible for Study:
60 Years to 70 Years (Adult, Older Adult)
Sexes Eligible for Study:
Accepts Healthy Volunteers:
Healthy females aged 60-70
Healthy post-menopausal females aged 60-70
• Obvious muscle wasting.
A body mass index (BMI) < 18 or > 40 kg•m2.
Active cardiovascular disease: uncontrolled high blood pressure, angina, heart failure (class III/IV), abnormal heart rhythm, right to left cardiac shunt or recent cardiac event.
Taking statin-based medication above 60mg•day-1.
Individuals taking beta-adrenergic blocking agents or Non-steroidal anti-inflammatory agents (NSAIDS)
Cerebrovascular disease: previous stroke, aneurysm (large vessel or intracranial).
Respiratory disease including pulmonary hypertension, chronic obstructive pulmonary disease (COPD), asthma or a forced expiratory volume (FEV1) less than 1.5 litres.
Metabolic disease: hyper and hypo parathyroidism, untreated hyper and hypothyroidism, Cushing's disease, types 1 or 2 diabetes.
Active inflammatory bowel disease, renal disease, or malignancy.
Recent steroid treatment (within 6 months), or hormone replacement therapy.
Clotting dysfunction e.g. Deep vein thrombosis, pulmonary embolus, warfarin therapy and/ or haemophilia.
Musculoskeletal or neurological disorders.
Any leg amputated
Family history of early (<55y) death from cardiovascular disease.